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Literature summary extracted from

  • Akiyama, Y.; Kihara, A.; Tokuda, H.; Ito, K.
    FtsH (HflB) is an ATP-dependent protease selectively acting on SecY and some other membrane proteins (1996), J. Biol. Chem., 271, 31196-31201.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B17 expression of wild-type and mutants as His-Myc-tagged proteins Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.B17 E415K site-directed mutagenesis, mutation of the zinc binding sequence motif, reduced proteolytic activity Escherichia coli
3.4.24.B17 K136N site-directed mutagenesis, mutation is located in a second ATP binding site, activity is slightly reduced, can complement a deficient mutant strain Escherichia coli
3.4.24.B17 K198N site-directed mutagenesis, mutation is located in the C-terminal ATP binding site, inactive, no complementation of a deficient mutant strain Escherichia coli
3.4.24.B17 S137N site-directed mutagenesis, mutation is located in a second ATP binding site, activity is slightly reduced, can complement a deficient mutant strain Escherichia coli
3.4.24.B17 T199A site-directed mutagenesis, mutation is located in the C-terminal ATP binding site, inactive, no complementation of a deficient mutant strain Escherichia coli
3.4.24.B17 T199N site-directed mutagenesis, mutation is located in the C-terminal ATP binding site, highly reduced activity, weak complementation of a deficient mutant strain Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.B17 membrane integral Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.B17 Zn2+ zinc metalloprotease, essential zinc binding sequence motif HEXXH at the cytoplasmic domain Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B17 protein + H2O Escherichia coli involved in membrane protein assembly as well as degradation of unstable proteins peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B17 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B17 recombinant His-Myc-tagged wild-type and mutants Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B17 protein + H2O
-
 Escherichia coli peptides
-
 ?
3.4.24.B17 protein + H2O involved in membrane protein assembly as well as degradation of unstable proteins Escherichia coli peptides
-
 ?
3.4.24.B17 protein SecY + H2O substrate protein is a subunit of protein translocase, failed to assemble with its partner SecE Escherichia coli ?
-
 ?
3.4.24.B17 subunit a of F0 + H2O F0 part of the proton ATPase Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B17 FtsH
-
 Escherichia coli
3.4.24.B17 HlB
-
 Escherichia coli
3.4.24.B17 M41.001 Merops-ID Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.B17 42
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.B17 7.5
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
3.4.24.B17 ATP dependent on, 2 ATP binding consensus sequences, 1 is located at the C-terminus and is essential for proteolytic activity Escherichia coli
3.4.24.B17 CTP ineffective substitute for ATP, low activity Escherichia coli
3.4.24.B17 UTP ineffective substitute for ATP, low activity Escherichia coli