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Literature summary extracted from

  • Knaeuper, V.; Lopez-Otin, C.; Smith, B.; Knight, G.; Murphy, G.
    Biochemical characterization of human collagenase-3 (1996), J. Biol. Chem., 271, 1544-1550.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B4 expression of procollagenase 3 in NSO mouse myeloma cells, secretion to the medium Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B4 hydroxamic acid-based inhibitor CT1399
-
Homo sapiens
3.4.24.B4 hydroxamic acid-based inhibitor CT1847
-
Homo sapiens
3.4.24.B4 tissue matrix metalloproteinase inhibitor TIMP-1 1:1 stoichiometric fashion Homo sapiens
3.4.24.B4 tissue matrix metalloproteinase inhibitor TIMP-2 1:1 stoichiometric fashion Homo sapiens
3.4.24.B4 tissue matrix metalloproteinase inhibitor TIMP-3 1:1 stoichiometric fashion Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.B4 additional information
-
additional information
-
Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.B4 48000
-
x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 48000, mature enzyme, SDS-PAGE Homo sapiens
3.4.24.B4 50000
-
x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 48000, mature enzyme, SDS-PAGE Homo sapiens
3.4.24.B4 60000
-
x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 48000, mature enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B4 protein + H2O Homo sapiens enzyme is involved in tumor progression, significant role in the turnover of connective tissue matrix constituents peptides
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B4 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.B4 glycoprotein N-glycosylated Homo sapiens
3.4.24.B4 proteolytic modification cleavage by trypsin is not effective, processing of the 60 kDa procollagenase 3 by stromelysin or by p-aminophenylmercuric acetate to form an intermediate of 50 kDa, further processing by cleavage of the peptide bond Glu84-Tyr85 resuls in the active 48 kDa mature form, overview Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B4 recombinant procollagenase 3 from NSO mouse myeloma cell culture medium Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.B4 breast carcinoma cell
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.B4 additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B4 (7-methoxycoumarin)-4-yl-acetyl-Pro-Cha-Gly-norvaline-His-Ala-N-3(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-NH2 + H2O
-
Homo sapiens (7-methoxycoumarin)-4-yl-acetyl-Pro-Cha-Gly + norvaline-His-Ala-N-3(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-NH2
-
?
3.4.24.B4 (7-methoxycoumarin)-4-yl-acetyl-Pro-Leu-Gly-Leu-N-3(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2 + H2O
-
Homo sapiens (7-methoxycoumarin)-4-yl-acetyl-Pro-Leu-Gly + Leu-N-3(2,4-dinitrophenyl)-L-2,3-diaminopropionyl-Ala-Arg-NH2
-
?
3.4.24.B4 alpha1-Antichymotrypsin + H2O cleavage of peptide bond Ala362-Leu363 Homo sapiens ?
-
?
3.4.24.B4 Collagen type I + H2O fibrillar, 5 to 6fold less active compared to substrate collagen type II Homo sapiens ?
-
?
3.4.24.B4 collagen type II + H2O soluble, preferred substrate Homo sapiens ?
-
?
3.4.24.B4 Collagen type III + H2O 5 to 6fold less active compared to substrate collagen type II Homo sapiens ?
-
?
3.4.24.B4 Gelatin + H2O
-
Homo sapiens ?
-
?
3.4.24.B4 additional information no activity with antithrombin III Homo sapiens ?
-
?
3.4.24.B4 plasminogen activator inhibitor 2 + H2O
-
Homo sapiens ?
-
?
3.4.24.B4 protein + H2O
-
Homo sapiens peptides
-
?
3.4.24.B4 protein + H2O enzyme is involved in tumor progression, significant role in the turnover of connective tissue matrix constituents Homo sapiens peptides
-
?

Subunits

EC Number Subunits Comment Organism
3.4.24.B4 ? x * 60000, proform, SDS-PAGE, x * 50000, intermediate partially processed form, SDS-PAGE, x * 48000, mature enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B4 M10.013 Merops-ID Homo sapiens
3.4.24.B4 matrix metalloproteinase-13
-
Homo sapiens
3.4.24.B4 MMP-13
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.B4 additional information
-
additional information
-
Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.B4 additional information
-
additional information
-
Homo sapiens