EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.B2 | gene pepF, DNA and amino acid sequence determination and analysis | Lactococcus lactis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.B2 | Zn2+ | zinc binding site, HEXXH motif | Lactococcus lactis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.B2 | 70000 | - |
x * 70000, SDS-PAGE | Lactococcus lactis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.B2 | protein + H2O | Lactococcus lactis | - |
peptides | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.B2 | Lactococcus lactis | P54124 | subsp. cremoris, i.e. Streptococcus cremoris, located on natural 55kb lactose-protease plasmid | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.B2 | to homogeneity, 75fold | Lactococcus lactis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.4.24.B2 | proteolytic cleavage of proteins at Leu-Gly | cleavage of Gly-/-Xaa, enzyme often cleaves Phe-/-Xaa, with X being hydrophilic or basic, and with proline in position P'2 of the cleaved bond, no cleavage of bonds involving acidic residues | Lactococcus lactis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.24.B2 | 22.42 | - |
purified enzyme | Lactococcus lactis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.B2 | adrenocorticotropic hormone fragment 1-10 + H2O | - |
Lactococcus lactis | SYSMEHF + RWG | - |
? | |
3.4.24.B2 | adrenocorticotropic hormone fragment 1-14 + H2O | - |
Lactococcus lactis | SYSMEHF + RWG + KPVG | - |
? | |
3.4.24.B2 | adrenocorticotropic hormone fragment 1-17 + H2O | - |
Lactococcus lactis | SYSMEHF + RWG + KPV + GKKR | - |
? | |
3.4.24.B2 | adrenocorticotropic hormone fragment 4-10 + H2O | - |
Lactococcus lactis | MEHF + RWG | - |
? | |
3.4.24.B2 | beta-casomorphin + H2O | - |
Lactococcus lactis | YPFP + GPI | - |
? | |
3.4.24.B2 | bradykinin + H2O | best substrate | Lactococcus lactis | RPPGF + SPR | - |
? | |
3.4.24.B2 | DesPro2bradykinin + H2O | - |
Lactococcus lactis | RPGF + SPFR | - |
? | |
3.4.24.B2 | insulin chain B fragment 22-30 + H2O | - |
Lactococcus lactis | RGFF + Tyr + TPKA | - |
? | |
3.4.24.B2 | Lys1bradykinin + H2O | - |
Lactococcus lactis | KPPGF + SPFR | - |
? | |
3.4.24.B2 | additional information | no activity with bradykinin fragment 2-10, Lys-p-nitroanilide, Gly-Pro-p-nitroanilide, adrenocorticotropic hormone fragment 1-4 SYSM and fragment 1-24, PQFY, Cbz-GPLGP, leucin enkephalin YGGFL, glucagon, full length insulin chain B, caseins | Lactococcus lactis | ? | - |
? | |
3.4.24.B2 | neurotensin + H2O | - |
Lactococcus lactis | Pyr-LYENKPR + RPYIL | - |
? | |
3.4.24.B2 | protein + H2O | - |
Lactococcus lactis | peptides | - |
? | |
3.4.24.B2 | protein + H2O | wide pH-dependent substrate specificity, cleaves peptides of length between 7 and 17 amino acids | Lactococcus lactis | peptides | - |
? | |
3.4.24.B2 | substance P fragment 2-11 + H2O | - |
Lactococcus lactis | PKPQQ + Phe + Phe + GLM-NH2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.B2 | ? | x * 70000, SDS-PAGE | Lactococcus lactis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.B2 | M03.007 | Merops-ID | Lactococcus lactis |
3.4.24.B2 | PepF | - |
Lactococcus lactis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.B2 | additional information | - |
substrate specificty is pH-dependent | Lactococcus lactis |