Literature summary extracted from
Kim, S.K.; Park, P.J.; Kim, J.B.; Shahidi, F.
Purification and characterization of a collagenolytic protease from the filefish, Novoden modestrus (2002), J. Biochem. Mol. Biol., 35, 165-171.
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.4.21.32 |
Cd2+ |
0.06 mM, 29% inhibition |
Novoden modestrus |
|
3.4.21.32 |
Cu2+ |
0.06 mM, 26% inhibition |
Novoden modestrus |
|
3.4.21.32 |
diisopropylfluorophosphate |
0.2 mM, complete inhibition |
Novoden modestrus |
|
3.4.21.32 |
EDTA |
0.2 mM, 46% inhibition. 0.5 mM, 95% inhibition |
Novoden modestrus |
|
3.4.21.32 |
Hg2+ |
0.06 mM, 19% inhibition |
Novoden modestrus |
|
3.4.21.32 |
His |
0.2 mM, 51% inhibition. 0.7 nmM, 93% inhibition |
Novoden modestrus |
|
3.4.21.32 |
Mn2+ |
0.06 mM, 14% inhibition |
Novoden modestrus |
|
3.4.21.32 |
Ni2+ |
0.06 mM, 30% inhibition |
Novoden modestrus |
|
3.4.21.32 |
Soybean trypsin inhibitor |
0.2 mM, complete inhibition |
Novoden modestrus |
|
3.4.21.32 |
tosyl-L-Leu-chloromethylketone |
0.2 mM, complete inhibition |
Novoden modestrus |
|
3.4.21.32 |
Zn2+ |
0.06 mM, 36% inhibition |
Novoden modestrus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.4.21.32 |
Ba2+ |
0.06 mM, activation to 119% of control |
Novoden modestrus |
|
3.4.21.32 |
Ca2+ |
0.06 mM, activation to 135% of control |
Novoden modestrus |
|
3.4.21.32 |
K+ |
0.06 mM, activation to 123% of control |
Novoden modestrus |
|
3.4.21.32 |
Li+ |
0.06 mM, activation to 111% of control |
Novoden modestrus |
|
3.4.21.32 |
Mg2+ |
0.06 mM, activation to 111% of control |
Novoden modestrus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.4.21.32 |
27000 |
- |
gel filtration |
Novoden modestrus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.21.32 |
Novoden modestrus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.4.21.32 |
- |
Novoden modestrus |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.4.21.32 |
10550 |
- |
- |
Novoden modestrus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.4.21.32 |
Collagen + H2O |
- |
Novoden modestrus |
Hydrolyzed collagen |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.4.21.32 |
monomer |
1 * 27000, SDS-PAGE |
Novoden modestrus |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.4.21.32 |
55 |
- |
hydrolysis of collagen |
Novoden modestrus |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
3.4.21.32 |
45 |
60 |
45°C: about 40% of maximal activity, 60°C: about 45% of maximal activity, hydrolysis of collagen |
Novoden modestrus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.4.21.32 |
7 |
8 |
hydrolysis of collagen |
Novoden modestrus |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.4.21.32 |
6.5 |
8.5 |
pH 6.5: about 60% of maximal activity, pH 8.5: about 50% of maximal activity, hydrolysis of collagen |
Novoden modestrus |