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Literature summary extracted from

  • Kaneko, Y.; Takeshita, H.; Mogi, K.; Nakajima, T.; Yasuda, T.; Itoi, M.; Kuwano, H.; Kishi, K.
    Molecular, biochemical and immunological analyses of canine pancreatic DNase I (2003), J. Biochem., 134, 711-718.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.1 DNA and amino acid sequence determination and analysis, expression in COS-7 cells Canis lupus familiaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.1 EDTA complete inhibition at 20 mM Canis lupus familiaris
3.1.21.1 EGTA complete inhibition at 5 mM Canis lupus familiaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.1 Ca2+ activates Canis lupus familiaris
3.1.21.1 Mg2+ activates Canis lupus familiaris
3.1.21.1 Mn2+ activates Canis lupus familiaris
3.1.21.1 additional information divalent cations are absolutely required for activity Canis lupus familiaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.1 additional information Canis lupus familiaris phylogenetic analysis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.1 Canis lupus familiaris Q767J3
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.21.1 glycoprotein 1 potential N-glycosylation site Canis lupus familiaris

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.1 260fold from pancreas, to homogeneity Canis lupus familiaris

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.21.1 endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products preference for double-stranded DNA Canis lupus familiaris
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.21.1 kidney low activity Canis lupus familiaris
-
3.1.21.1 large intestine low activity Canis lupus familiaris
-
3.1.21.1 liver low activity Canis lupus familiaris
-
3.1.21.1 additional information no activity in spleen, parotid gland, lung, heart, cerebellum, and cerebrum Canis lupus familiaris
-
3.1.21.1 pancreas highest enzyme activity and expression Canis lupus familiaris
-
3.1.21.1 small intestine low activity Canis lupus familiaris
-
3.1.21.1 stomach
-
 Canis lupus familiaris
-
3.1.21.1 sublingual gland low activity Canis lupus familiaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.21.1 additional information
-
 purified enzyme Canis lupus familiaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.1 DNA + H2O
-
 Canis lupus familiaris 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
 ?
3.1.21.1 additional information phylogenetic analysis Canis lupus familiaris ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.1 DNase I
-
 Canis lupus familiaris
3.1.21.1 pancreatic DNase I
-
 Canis lupus familiaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.1 6.5
-
-
 Canis lupus familiaris