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Literature summary extracted from

  • Sugihara, A.; Shimada, Y.; Sugihara, S.; Nagao, T.; Watanabe, Y.; Tominaga, Y.
    A novel alpha-amino-acid esterase from Bacillus mycoides capable of forming peptides of DD- and DL-configurations (2001), J. Biochem., 130, 119-126.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.43 phenylmethanesulfonyl fluoride 1 mM, 99% inhibition Bacillus mycoides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.43 39000
-
4 * 39000, SDS-PAGE Bacillus mycoides
3.1.1.43 160000
-
gel filtration Bacillus mycoides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.43 an alpha-aminoacid ester + H2O Bacillus mycoides
-
an alpha-amino acid + an alcohol
-
r

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.43 Bacillus mycoides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.43 ammonium sulfate, DEAE-Toyopearl, electrofocusing, Disc electrophoresis Bacillus mycoides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.43 2160
-
-
Bacillus mycoides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.43 acetyl-D-phenyl methyl ester + L-leucine amide
-
Bacillus mycoides acetyl-D-phenyl-L-leucine + methanol
-
?
3.1.1.43 acetyl-D-phenyl methyl ester + L-phenylalanine amide
-
Bacillus mycoides acetyl-D-phenyl-L-phenylalanine + methanol
-
?
3.1.1.43 acetyl-D-phenylalanine methyl ester + H2O
-
Bacillus mycoides acetyl-D-phenylalanine + methanol
-
r
3.1.1.43 acetyl-L-phenylalanine methyl ester + H2O
-
Bacillus mycoides acetyl-L-phenylalanine + methanol
-
r
3.1.1.43 an alpha-aminoacid ester + H2O
-
Bacillus mycoides an alpha-amino acid + an alcohol
-
?
3.1.1.43 an alpha-aminoacid ester + H2O
-
Bacillus mycoides an alpha-amino acid + an alcohol
-
r
3.1.1.43 D-aspartate dimethyl ester + H2O
-
Bacillus mycoides D-aspartate + methanol
-
r
3.1.1.43 D-methionine methyl ester + H2O
-
Bacillus mycoides D-methionine + methanol
-
r
3.1.1.43 D-phenylalanine amide + H2O
-
Bacillus mycoides D-phenylalanine + NH3
-
?
3.1.1.43 D-phenylalanine methyl ester + H2O
-
Bacillus mycoides D-phenylalanine + methanol
-
r
3.1.1.43 D-tryptophan methyl ester + H2O
-
Bacillus mycoides D-tryptophan + methanol
-
r
3.1.1.43 D-tyrosine methyl ester + H2O
-
Bacillus mycoides D-tyrosine + methanol
-
r
3.1.1.43 L-aspartate dimethyl ester + H2O
-
Bacillus mycoides L-aspartate + methanol
-
?
3.1.1.43 L-methionine methyl ester + H2O
-
Bacillus mycoides L-methionine + methanol
-
r
3.1.1.43 L-phenylalanine methyl ester + H2O
-
Bacillus mycoides L-phenylalanine + methanol
-
r
3.1.1.43 L-tryptophan methyl ester + H2O
-
Bacillus mycoides L-tryptophan + methanol
-
r
3.1.1.43 L-tyrosine methyl ester + H2O
-
Bacillus mycoides L-tyrosine + methanol
-
r
3.1.1.43 methyl acetate 1.1% of activity with D-phenylalanine methylester Bacillus mycoides acetate + methanol
-
?
3.1.1.43 methyl butyrate 3.6% of activity with D-phenylalanine methylester Bacillus mycoides butyrate + methanol
-
?
3.1.1.43 methyl caproate 2% of activity with D-phenylalanine methylester Bacillus mycoides caproate + methanol
-
?
3.1.1.43 methyl caprylate 1.1% of activity with D-phenylalanine methylester Bacillus mycoides caprylate + methanol
-
?
3.1.1.43 methyl propionate 2.7% of activity with D-phenylalanine methylester Bacillus mycoides propionate + methanol
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.43 tetramer 4 * 39000, SDS-PAGE Bacillus mycoides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.43 47
-
-
Bacillus mycoides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.43 10 65 40% of maximal activity at 10°C, approx. 5% of maximal activity at 65°C Bacillus mycoides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.43 6160
-
phenylalanine methylester 25°C, pH 7.0 Bacillus mycoides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.43 7.6
-
hydrolysis of phenylalanine methylester Bacillus mycoides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.43 4 11 approx. 10% of maximal activity at pH 4.0 and pH 10.0, respectively Bacillus mycoides

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.43 Bacillus mycoides isoelectric focusing
-
7