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Literature summary extracted from

  • Kitaura, S.; Suzuki, K.; Imamura, S.
    Monoacylglycerol lipase from moderately thermophilic Bacillus sp. strain H-257: molecular cloning, sequencing, and expression in Escherichia coli of the gene (2001), J. Biochem., 129, 397-402.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.23 expressed in Escherichia coli Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.23 27400
-
 calculated from amino acid sequence Bacillus sp. (in: Bacteria)
3.1.1.23 28000
-
 SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.23 Bacillus sp. (in: Bacteria) P82597 H-257
-
3.1.1.23 Bacillus sp. (in: Bacteria) H-257 P82597 H-257
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.23 1-monocapryloylglycerol + H2O best substrate Bacillus sp. (in: Bacteria) caprylic acid + glycerol
-
 ?
3.1.1.23 1-monocapryloylglycerol + H2O best substrate Bacillus sp. (in: Bacteria) H-257 caprylic acid + glycerol
-
 ?
3.1.1.23 1-monolauroylglycerol + H2O good substrate Bacillus sp. (in: Bacteria) lauric acid + glycerol
-
 ?
3.1.1.23 1-monolauroylglycerol + H2O good substrate Bacillus sp. (in: Bacteria) H-257 lauric acid + glycerol
-
 ?
3.1.1.23 1-monolinoleoylycerol + H2O
-
 Bacillus sp. (in: Bacteria) linoleic acid + glycerol
-
 ?
3.1.1.23 1-monomyristoylglycerol + H2O good substrate Bacillus sp. (in: Bacteria) myristic acid + glycerol
-
 ?
3.1.1.23 1-monomyristoylglycerol + H2O good substrate Bacillus sp. (in: Bacteria) H-257 myristic acid + glycerol
-
 ?
3.1.1.23 1-monooleoylglycerol + H2O
-
 Bacillus sp. (in: Bacteria) oleic acid + glycerol
-
 ?
3.1.1.23 1-monooleoylglycerol + H2O
-
 Bacillus sp. (in: Bacteria) H-257 oleic acid + glycerol
-
 ?
3.1.1.23 1-monopalmitoylglycerol + H2O
-
 Bacillus sp. (in: Bacteria) palmitic acid + glycerol
-
 ?
3.1.1.23 1-monopalmitoylglycerol + H2O
-
 Bacillus sp. (in: Bacteria) H-257 palmitic acid + glycerol
-
 ?
3.1.1.23 1-monostearoylglycerol + H2O
-
 Bacillus sp. (in: Bacteria) stearic acid + glycerol
-
 ?
3.1.1.23 4-nitrophenyl butyrate + H2O
-
 Bacillus sp. (in: Bacteria) 4-nitrophenol + butyric acid
-
 ?
3.1.1.23 4-nitrophenyl caprylate + H2O
-
 Bacillus sp. (in: Bacteria) 4-nitrophenol + caprylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.23 MGLP
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.23 75
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.23 60
-
 stable below Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.23 6 8
-
 Bacillus sp. (in: Bacteria)

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.23 Bacillus sp. (in: Bacteria) isoelectric focusing 4.8 4.7