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Literature summary extracted from

  • Hattori, A.; Kitatani, K.; Matsumoto, H.; Miyazawa, S.; Rogi, T.; Tsuruoka, N.; Mizutani, S.; Natori, Y.; Tsujimoto, M.
    Characterization of recombinant human adipocyte-derived leucine aminopeptidase expressed in chinese hamster ovary cells (2000), J. Biochem., 128, 755-762.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 expression in COS-7 and in CHO cells, secretion from recombinant COS-7 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 amastatin
-
Homo sapiens
3.4.11.1 Zn2+
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 1.28
-
Leu-4-nitroanilide
-
Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Zinc zinc-metallopeptidase, contains the HEXXH motif Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 106000
-
1 * 106000, recombinant enzyme, SDS-PAGE Homo sapiens
3.4.11.1 120000
-
recombinant enzyme from CHO cells, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.1 angiotensin II + H2O Homo sapiens
-
angiotensin IV + ? through angiotensin III ?
3.4.11.1 kallidin + H2O Homo sapiens i.e. LRPPGFSPFR Leu + bradykinin i.e. RPPGFSPFR ?
3.4.11.1 additional information Homo sapiens broad substrate specificity towards naturally occurring peptide hormones, while the enzyme hydrolyzes synthetic substrate in vitro with specificity for leucine, enzyme plays a role in regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 recombinant from CHO cells, 5fold to homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 adipocyte
-
Homo sapiens
-
3.4.11.1 kidney cortex Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.1 0.192
-
purified recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 angiotensin II + H2O
-
Homo sapiens angiotensin IV + ? through angiotensin III ?
3.4.11.1 dynorphin A + H2O
-
Homo sapiens ?
-
?
3.4.11.1 kallidin + H2O i.e. LRPPGFSPFR Homo sapiens Leu + bradykinin i.e. RPPGFSPFR ?
3.4.11.1 Leu-4-nitroanilide + H2O
-
Homo sapiens Leu + 4-nitroaniline
-
?
3.4.11.1 additional information broad substrate specificity towards naturally occurring peptide hormones, while the enzyme hydrolyzes synthetic substrate in vitro with specificity for leucine, enzyme plays a role in regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney Homo sapiens ?
-
?
3.4.11.1 Neurokinin A + H2O
-
Homo sapiens ?
-
?
3.4.11.1 Neuromedin B + H2O
-
Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 monomer 1 * 106000, recombinant enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 A-LAP
-
Homo sapiens
3.4.11.1 adipocyte-derived leucine aminopeptidase
-
Homo sapiens
3.4.11.1 aminopeptidase I
-
Homo sapiens
3.4.11.1 aminopeptidase II
-
Homo sapiens
3.4.11.1 Aminopeptidase III
-
Homo sapiens
3.4.11.1 cathepsin III
-
Homo sapiens
3.4.11.1 cytosol aminopeptidase
-
Homo sapiens
3.4.11.1 FTBL protein
-
Homo sapiens
3.4.11.1 L-leucine aminopeptidase
-
Homo sapiens
3.4.11.1 leucinamide aminopeptidase
-
Homo sapiens
3.4.11.1 leucinaminopeptidase
-
Homo sapiens
3.4.11.1 leucine aminopeptidase
-
Homo sapiens
3.4.11.1 leucyl peptidase
-
Homo sapiens
3.4.11.1 More enzyme belongs to the peptidase family M17, enzyme belongs to the metallopeptidase family M1 Homo sapiens
3.4.11.1 peptidase S
-
Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 7.5
-
-
Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.1 additional information
-
pH profile Homo sapiens