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Literature summary extracted from

  • Takahashi, S.; Takahashi, K.; Kaneko, T.; Ogasawara, H.; Shindo, S.; Saito, K.; Kobayashi, M.
    Identification of cysteine-380 as the essential residue for the human N-acetyl-D-glucosamine 2-epimerase (renin binding protein) (1999), J. Biochem., 126, 639-642.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.8 expression of wild-type enzyme and mutant enzymes C41S, C66S, C104S, C125S, C210S, C239S, C302S, C380S, C386S and C390S in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.8 C104S specific activity in the extract is 25% of that of the wild-type enzyme Homo sapiens
5.1.3.8 C125S relative specific activity in the extract is nearly the same to that of the wild-type enzyme Homo sapiens
5.1.3.8 C210S relative specific activity in the extract is nearly the same to that of the wild-type enzyme Homo sapiens
5.1.3.8 C239S relative specific activity in the extract is nearly the same to that of the wild-type enzyme Homo sapiens
5.1.3.8 C302S relative specific activity in the extract is nearly the same to that of the wild-type enzyme Homo sapiens
5.1.3.8 C380S no enzyme activity detected in the extract Homo sapiens
5.1.3.8 C386S relative specific activity in the extract is nearly the same to that of the wild-type enzyme Homo sapiens
5.1.3.8 C390S relative specific activity in the extract is nearly the same to that of the wild-type enzyme Homo sapiens
5.1.3.8 C41S relative specific activity in the extract is nearly the same to that of the wild-type enzyme Homo sapiens
5.1.3.8 C66S relative specific activity in the extract is nearly the same to that of the wild-type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.8 5,5'-dithiobis-2-nitrobenzoate 0.1 mM, more than 95% inhibition Homo sapiens
5.1.3.8 Monoiodoacetic acid 10 mM, more than 95% inhibition Homo sapiens
5.1.3.8 NaN3 10 mM, 18% inhibition Homo sapiens
5.1.3.8 NEM 1 mM, more than 95% inhibition Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.8 82000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.8 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.8 N-acetyl-D-mannosamine
-
 Homo sapiens N-acetyl-D-glucosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.1.3.8 renin binding protein
-
 Homo sapiens
5.1.3.8 RNBP
-
 Homo sapiens