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Literature summary extracted from

  • Takahashi, S.; Takahashi, K.; Kaneko, T.; Ogasawara, H.; Shindo, S.; Kobayashi, M.
    Human renin-binding protein is the enzyme N-acetyl-D-glucosamine 2-epimerase (1999), J. Biochem., 125, 348-353.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.3.8 ATP required, Km: 0.13 mM Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.8 expressed in Escherichia coli JM109 under the transcriptional control of taq promoter Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.8 5,5'-dithiobis(2-nitrobenzoic acid) 0.1 mM, more than 95% inhibition Homo sapiens
5.1.3.8 Monoiodoacetic acid 10 mM, more than 95% inhibition Homo sapiens
5.1.3.8 NEM 1 mM, more than 95% inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.8 12.8
-
 N-acetyl-D-mannosamine 37°C, pH 7.5 Homo sapiens
5.1.3.8 21.3
-
 N-acetyl-D-glucosamine 37°C, pH 7.5 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.8 45000
-
 2 * 45000, SDS-PAGE Homo sapiens
5.1.3.8 85000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.8 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.8
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.8 35.8
-
 37°C, pH 7.5 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.8 additional information no activity with mannosamine, glucosamine, mannose, glucose and N-acetyl-galactosamine Homo sapiens ?
-
 ?
5.1.3.8 additional information the enzyme inhibits porcine renin activity through formation of a complex of porcine renin with the recombinant enzyme, the so-called high-molecular-weight renin Homo sapiens ?
-
 ?
5.1.3.8 N-acetyl-D-mannosamine
-
 Homo sapiens N-acetyl-D-glucosamine
-
 r

Subunits

EC Number Subunits Comment Organism
5.1.3.8 dimer 2 * 45000, SDS-PAGE Homo sapiens