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Literature summary extracted from

  • Fukui, T.; Eguchi, T.; Atomi, H.; Imanaka, T.
    A membrane-bound archaeal Lon protease displays ATP-independent proteolytic activity towards unfolded proteins and ATP-dependent activity for folded proteins (2002), J. Bacteriol., 184, 3689-3698.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.53 LonTK cloned and expressed in Escherichia coli Thermococcus kodakarensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.53 membrane membrane-bound Thermococcus kodakarensis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.53 Ca2+
-
 Thermococcus kodakarensis
3.4.21.53 Co2+ supports the hydrolysis Thermococcus kodakarensis
3.4.21.53 Mg2+
-
 Thermococcus kodakarensis
3.4.21.53 Mn2+ supports the hydrolysis Thermococcus kodakarensis
3.4.21.53 Ni2+ serves as a cofactor better than Mg2+ and Ca2+ Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.53 70000
-
 SDS-PAGE Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.53 Thermococcus kodakarensis Q8NKS6 KOD1, previously called Pyrococcus kodakaraensis
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.53 recombinant enzyme Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.53 ATP + H2O
-
 Thermococcus kodakarensis phosphate + ADP
-
 ?
3.4.21.53 glutaryl-Ala-Ala-Phe-4-methoxy-beta-naphthylamide + H2O
-
 Thermococcus kodakarensis ?
-
 ?
3.4.21.53 hemoglobin A + H2O can degrade unfolded human hemoglobin A at 70°C either in presence or absence of ATP, at 37°C only in presence of ATP Thermococcus kodakarensis ?
-
 ?
3.4.21.53 additional information belonging to AAA+ superfamily Thermococcus kodakarensis ?
-
 ?
3.4.21.53 ribulose-1,5-bisphosphate carboxylase/oxygenase + H2O RubiscoTK Thermococcus kodakarensis ?
-
 ?
3.4.21.53 succinyl-Phe-Leu-Phe-4-methoxy-beta-naphthylamide + H2O
-
 Thermococcus kodakarensis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.53 archaeal Lon protease
-
 Thermococcus kodakarensis
3.4.21.53 lon protease
-
 Thermococcus kodakarensis
3.4.21.53 lonTK
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.53 70
-
 peptide cleavage activity Thermococcus kodakarensis
3.4.21.53 95
-
 ATPase activity Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.53 9
-
-
 Thermococcus kodakarensis