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Literature summary extracted from

  • Verhees, C.H.; Akerboom, J.; Schiltz, E.; de Vos, W.M.; van der Oost, J.
    Molecular and biochemical characterization of a distinct type of fructose-1,6-bisphosphatase from Pyrococcus furiosus (2002), J. Bacteriol., 184, 3401-3405.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.11 expression in Escherichia coli Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 ADP
-
 Pyrococcus furiosus
3.1.3.11 ATP
-
 Pyrococcus furiosus
3.1.3.11 D-glucose 6-phosphate
-
 Pyrococcus furiosus
3.1.3.11 Li+
-
 Pyrococcus furiosus
3.1.3.11 additional information Na+, K+, phosphoenolpyruvate Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.31
-
 D-fructose 1,6-bisphosphate pH 8.0, 50°C Pyrococcus furiosus
3.1.3.11 0.32
-
 D-fructose 1,6-bisphosphate pH 8.0, 22°C Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 Mg2+ required Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.11 27900
-
 2 * 33000, gel filtration, 2 * 27900, SDS-PAGE Pyrococcus furiosus
3.1.3.11 33000
-
 2 * 33000, gel filtration, 2 * 27900, SDS-PAGE Pyrococcus furiosus
3.1.3.11 66800
-
 gel filtartion, recombinant enzyme Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Pyrococcus furiosus enzyme is usually regarded as a regulatory enzyme of gluconeogenesis D-fructose 6-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Pyrococcus furiosus Q8TZH9
-
-
3.1.3.11 Sus scrofa P00636
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11 the recombinant enzyme Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.11 additional information
-
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Pyrococcus furiosus D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O enzyme is usually regarded as a regulatory enzyme of gluconeogenesis Pyrococcus furiosus D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-glucose-1-phosphate + H2O
-
 Pyrococcus furiosus D-glucose + phosphate
-
 ?
3.1.3.11 glycerol-2-phosphate + H2O
-
 Pyrococcus furiosus glycerol + phosphate
-
 ?
3.1.3.11 myo-inositol 1-phosphate + H2O
-
 Pyrococcus furiosus myo-inositol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.11 dimer 2 * 33000, gel filtration, 2 * 27900, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.11 FBPase I
-
-
3.1.3.11 FBPase IV the enzyme is present in euryarchaeal and hyperthermophilic bacterial species Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.11 100
-
 pH 7.4 Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.11 0.3
-
 D-fructose 1,6-bisphosphate pH 8.0, 50°C Pyrococcus furiosus
3.1.3.11 18
-
 D-fructose 1,6-bisphosphate pH 8.0, 22°C Pyrococcus furiosus