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Literature summary extracted from

  • Morty, R.E.; Fueloep, V.; Andrews, N.W.
    Substrate recognition properties of oligopeptidase B from Salmonella enterica serovar Typhimurium (2002), J. Bacteriol., 184, 3329-3337.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.21.83 medicine potential theraputic target in infectious disease Salmonella enterica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.83 opdB gene PCR amplified and expressed in Escherichia coli BL21(lambdaDE3) Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.83 D460T site-mutated variant Salmonella enterica
3.4.21.83 D460T/D462N site-mutated variant Salmonella enterica
3.4.21.83 D462N site-mutated variant Salmonella enterica
3.4.21.83 D567H site-mutated variant Salmonella enterica
3.4.21.83 D599H site-mutated variant Salmonella enterica
3.4.21.83 D638Q site-mutated variant Salmonella enterica
3.4.21.83 E494H site-mutated variant Salmonella enterica
3.4.21.83 E576A site-mutated variant Salmonella enterica
3.4.21.83 E576A/E578A site-mutated variant Salmonella enterica
3.4.21.83 E576R/E578R site-mutated variant Salmonella enterica
3.4.21.83 E576W/E578T site-mutated variant Salmonella enterica
3.4.21.83 E578A site-mutated variant Salmonella enterica
3.4.21.83 E624H site-mutated variant Salmonella enterica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.83 3,4-dichloroisocoumarin irreversible inhibition Salmonella enterica
3.4.21.83 4-(2-aminoethyl)benzenesulfonyl fluoride irreversible inhibition Salmonella enterica
3.4.21.83 antipain reversible inhibition Salmonella enterica
3.4.21.83 benzamidine reversible inhibition Salmonella enterica
3.4.21.83 leupeptin reversible inhibition Salmonella enterica
3.4.21.83 additional information no inhibition of wild-type enzyme with iodoacetamide, iodoacetate, N-ethylmaleimide, EDTA, EGTA, bestatin,amastatin, arphamenine A, elastinal, dynorphin A or dynorphin B Salmonella enterica
3.4.21.83 p-aminobenzamidine reversible inhibition Salmonella enterica
3.4.21.83 phenylmethane sulfonyl fluoride irreversible inhibition Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.83 0.0008
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, wild-type and mutant D567H Salmonella enterica
3.4.21.83 0.00088
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, opdB with polyhistidine affinty tag removed Salmonella enterica
3.4.21.83 0.001
-
 Boc-Leu-Lys-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.001
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E494H, E624H, and D599H Salmonella enterica
3.4.21.83 0.0011
-
 Boc-Leu-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.0011
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D638Q Salmonella enterica
3.4.21.83 0.0019
-
 Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.0019
-
 Boc-Gly-Lys-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.002
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D567H Salmonella enterica
3.4.21.83 0.0021
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T Salmonella enterica
3.4.21.83 0.0021
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, wild-type and mutant D599A Salmonella enterica
3.4.21.83 0.0021
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, wild-type and mutant D599H Salmonella enterica
3.4.21.83 0.0022
-
 Boc-Leu-Thr-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.0023
-
 benzyloxycarbonyl-Gly-Gly-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.0023
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D462N Salmonella enterica
3.4.21.83 0.0024
-
 Boc-Leu-Gly-Lys-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.0025
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E494H Salmonella enterica
3.4.21.83 0.0026
-
 benzyloxycarbonyl-Val-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.0026
-
 Boc-Leu-Gly-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.0026
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E624H Salmonella enterica
3.4.21.83 0.0028
-
 Boc-Gln-Gly-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.0028
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D638Q Salmonella enterica
3.4.21.83 0.0029
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T and D460T/D462N Salmonella enterica
3.4.21.83 0.0029
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T/D462N Salmonella enterica
3.4.21.83 0.003
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D462N Salmonella enterica
3.4.21.83 0.0034
-
 benzyloxycarbonyl-Gly-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.004
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D638Q Salmonella enterica
3.4.21.83 0.0044
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.0045
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D599H Salmonella enterica
3.4.21.83 0.0045
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E576A Salmonella enterica
3.4.21.83 0.0046
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T and E494H Salmonella enterica
3.4.21.83 0.0047
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D462N Salmonella enterica
3.4.21.83 0.005
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D567H Salmonella enterica
3.4.21.83 0.0051
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, opdB with polyhistidine affinty tag removed Salmonella enterica
3.4.21.83 0.0052
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T/D462N Salmonella enterica
3.4.21.83 0.0054
-
 benzyloxycarbonyl-Lys-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.0054
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E624H Salmonella enterica
3.4.21.83 0.0059
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E578A Salmonella enterica
3.4.21.83 0.0081
-
 Tos-Gly-Pro-Lys-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.0099
-
 Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.012
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E576A Salmonella enterica
3.4.21.83 0.0164
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E578A Salmonella enterica
3.4.21.83 0.0334
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E576A Salmonella enterica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.83 78970
-
 predicted from opdB gene open reading frame Salmonella enterica

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.83 Salmonella enterica A0A0H3NCA6 serovar typhimurium, virulent wild-type strain SL1344
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.83 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
3.4.21.83 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
3.4.21.83 benzyloxycarbonyl-Gly-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
3.4.21.83 benzyloxycarbonyl-Gly-Gly-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
3.4.21.83 benzyloxycarbonyl-Lys-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
3.4.21.83 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
3.4.21.83 benzyloxycarbonyl-Val-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
3.4.21.83 Boc-Gln-Gly-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
3.4.21.83 Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica Boc-Gly-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 Boc-Gly-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica Boc-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.83 Boc-Leu-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
3.4.21.83 Boc-Leu-Gly-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
3.4.21.83 Boc-Leu-Gly-Lys-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
3.4.21.83 Boc-Leu-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
3.4.21.83 Boc-Leu-Thr-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
3.4.21.83 histone + H2O human histones H1, H2A, H3 and H4, restricted proteolysis Salmonella enterica ?
-
 ?
3.4.21.83 additional information exhibits amidolytic activity exclusively against substrates with basic residues in P1, no activity is observed against H-Arg-4-methylcoumarin 7-amide, H-Leu-4-methylcoumarin 7-amide, H-Lys-4-methylcoumarin 7-amide, benzyloxycarbonyl-Leu-Leu-Glu-beta-nitroanilide, benzyloxycarbonyl-Gly-Gly-Leu-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Phe-4-methylcoumarin 7-amide, Suc-Ile-Ala-4-methylcoumarin 7-amide, Suc-Gly-Pro-4-methylcoumarin 7-amide, Ac-Tyr-Val-Ala-Asp-p-nitroanilide, Suc-Ala-Ala-Pro-Phe-4-methylcoumarin 7-amide Salmonella enterica ?
-
 ?
3.4.21.83 Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
3.4.21.83 Tos-Gly-Pro-Lys-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.83 OpdB
-
 Salmonella enterica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.83 120
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E576A Salmonella enterica
3.4.21.83 240
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E578A Salmonella enterica
3.4.21.83 420
-
 benzyloxycarbonyl-Lys-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 420
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T/D462N Salmonella enterica
3.4.21.83 420
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E576A Salmonella enterica
3.4.21.83 540
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E576A and E578A Salmonella enterica
3.4.21.83 540
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E578A Salmonella enterica
3.4.21.83 540
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, wild-type, mutant E494H, D599H and D567H Salmonella enterica
3.4.21.83 600
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, opdB with polyhistidine affinity tag removed, mutant D460T, D462N and E624H Salmonella enterica
3.4.21.83 660
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D638Q Salmonella enterica
3.4.21.83 780
-
 Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 900
-
 benzyloxycarbonyl-Val-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 900
-
 benzyloxycarbonyl-Gly-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 960
-
 Boc-Leu-Gly-Lys-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 1020
-
 Boc-Gln-Gly-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 1020
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T/D462N Salmonella enterica
3.4.21.83 1080
-
 Boc-Leu-Thr-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 1080
-
 Boc-Leu-Gly-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 1080
-
 Tos-Gly-Pro-Lys-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 1080
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T and E494H Salmonella enterica
3.4.21.83 1080
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T/D462N Salmonella enterica
3.4.21.83 1140
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D462N Salmonella enterica
3.4.21.83 1200
-
 Boc-Gly-Lys-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 1200
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D599A and D638Q Salmonella enterica
3.4.21.83 1260
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 1320
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T Salmonella enterica
3.4.21.83 1320
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D567H and E624H Salmonella enterica
3.4.21.83 1380
-
 Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 1380
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D462N Salmonella enterica
3.4.21.83 1500
-
 benzyloxycarbonyl-Gly-Gly-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 1560
-
 Boc-Leu-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
3.4.21.83 1740
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C wild-type and mutant D567H Salmonella enterica
3.4.21.83 1800
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D599H Salmonella enterica
3.4.21.83 1860
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D638Q Salmonella enterica
3.4.21.83 1980
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E494H and E624H Salmonella enterica
3.4.21.83 2040
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, opdB with polyhistidine affinty tag removed Salmonella enterica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.83 0.0000081
-
 antipain pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.0004
-
 leupeptin pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.1171
-
 p-aminobenzamidine pH 8.0, 37°C Salmonella enterica
3.4.21.83 0.1867
-
 benzamidine pH 8.0, 37°C Salmonella enterica