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Literature summary extracted from

  • Zilles, J.L.; Kappock, T.J.; Stubbe, J.; Downs, D.M.
    Altered pathway routing in a class of Salmonella enterica serovar Typhimurium mutants defective in aminoimidazole ribonucleotide synthetase (2001), J. Bacteriol., 183, 2234-2240.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.3.1 expression in Escherichia coli of a histidine-tagged enzyme Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
6.3.3.1 additional information double mutant R35C/E57G obtained by site-directed mutagenesis Salmonella enterica
6.3.3.1 additional information triple mutant R35C/E57G/R198W obtained by site-directed mutagenesis Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.3.1 0.049
-
 2-(formamido)-N1-(5-phosphoribosyl)acetamidine 37°C, pH 7.7, wild type enzyme Salmonella enterica
6.3.3.1 0.066
-
 2-(formamido)-N1-(5-phosphoribosyl)acetamidine 37°C, pH 7.7, R35C/E57G mutant enzyme Salmonella enterica
6.3.3.1 0.126
-
 ATP 37°C, pH 7.7, wild type enzyme Salmonella enterica
6.3.3.1 1.68
-
 ATP 37°C, pH 7.7, R35C/E57G mutant enzyme Salmonella enterica

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.1 Salmonella enterica P74883 serovar typhimurium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.1 purification of a histidine-tagged enzyme by nickel-affinity column chromatography Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.1 ATP + 2-(formamido)-N1-(5-phosphoribosyl)acetamidine
-
 Salmonella enterica ADP + phosphate + 1-(5-phosphoribosyl)-5-aminoimidazole
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.3.1 aminoimidazole ribonucleotide synthetase
-
 Salmonella enterica