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Literature summary extracted from

  • Yoshida, K.I.; Fujita, Y.; Ehrlich, S.D.
    Three asparagine synthetase genes of Bacillus subtilis (1999), J. Bacteriol., 181, 6081-6091.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.4 three asparagine snythetase genes asnB, asnH and asnO, expression in Bacillus subtilis and in Escherichia coli ME6279, sequencing, genetic organization Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.4 additional information various mutant strains, deletion of asnO or asnH, singly or in combination, has no effect on growth rates in media with or without asparagine, deletion of asnB leads to a slow-growth phenotype, even in the presence of asparagine, strains lacking asnO cannot sporulate Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.4 additional information Bacillus subtilis physiological roles for asnB in vegetative cells and for asnO in sporulating cells, asnB may be the main gene involved in asparagine biosynthesis ?
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 ?
6.3.5.4 additional information Bacillus subtilis 168 physiological roles for asnB in vegetative cells and for asnO in sporulating cells, asnB may be the main gene involved in asparagine biosynthesis ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Bacillus subtilis
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 three glutamine-dependent AsnB-type asparagine snythetase genes: asnB, asnH and asnO, formerly yisO
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6.3.5.4 Bacillus subtilis 168
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 three glutamine-dependent AsnB-type asparagine snythetase genes: asnB, asnH and asnO, formerly yisO
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.4 additional information the three asparagine synthetase genes asnB, asnH and asnO are differentially expressed during cell growth, expression pattern Bacillus subtilis
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-aspartate + L-glutamine
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 Bacillus subtilis AMP + diphosphate + L-asparagine + L-glutamate
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 ?
6.3.5.4 ATP + L-aspartate + L-glutamine
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 Bacillus subtilis 168 AMP + diphosphate + L-asparagine + L-glutamate
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 ?
6.3.5.4 additional information physiological roles for asnB in vegetative cells and for asnO in sporulating cells, asnB may be the main gene involved in asparagine biosynthesis Bacillus subtilis ?
-
 ?
6.3.5.4 additional information physiological roles for asnB in vegetative cells and for asnO in sporulating cells, asnB may be the main gene involved in asparagine biosynthesis Bacillus subtilis 168 ?
-
 ?