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Literature summary extracted from

  • Musfeldt, M.; Selig, M.; Schonheit, P.
    Acetyl coenzyme A synthetase (ADP forming) from the hyperthermophilic Archaeon pyrococcus furiosus: identification, cloning, separate expression of the encoding genes, acdAI and acdBI, in Escherichia coli, and in vitro reconstitution of the active heterotetrameric enzyme from its recombinant subunits (1999), J. Bacteriol., 181, 5885-5888.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.13 expression in Escherichia coli Giardia intestinalis
6.2.1.13 overexpression in Escherichia coli Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.13 0.019
-
 acetyl-CoA
-
 Pyrococcus furiosus
6.2.1.13 0.021
-
 CoA
-
 Pyrococcus furiosus
6.2.1.13 0.06
-
 ADP
-
 Pyrococcus furiosus
6.2.1.13 0.06
-
 acetyl-CoA pH 7.5, at 30ºC Giardia intestinalis
6.2.1.13 0.09
-
 ATP
-
 Pyrococcus furiosus
6.2.1.13 0.1
-
 phosphate
-
 Pyrococcus furiosus
6.2.1.13 0.2
-
 ADP pH 7.5, at 30ºC Giardia intestinalis
6.2.1.13 0.8
-
 acetate
-
 Pyrococcus furiosus
6.2.1.13 1.35
-
 phosphate pH 7.5, at 30ºC Giardia intestinalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.13 soluble
-
 Giardia intestinalis
-
-
6.2.1.13 soluble
-
 Pyrococcus furiosus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.13 additional information requirement for a divalent cation Giardia intestinalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.13 25000
-
 alpha2,beta2, 2 * 47000 (alpha) + 2 * 25000 (beta), SDS-PAGE Pyrococcus furiosus
6.2.1.13 47000
-
 alpha2,beta2, 2 * 47000 (alpha) + 2 * 25000 (beta), SDS-PAGE Pyrococcus furiosus
6.2.1.13 145000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.13 ADP + phosphate + acetyl-CoA Giardia intestinalis enzyme catalyzes acetate formation and ATP generation during fermentation of pyruvate to acetate ATP + acetate + CoA
-
 ?
6.2.1.13 ADP + phosphate + acetyl-CoA Pyrococcus furiosus major energy-conserving reaction during pyruvate and sugar conversion to acetate, catalyzing acetate formation and ATP synthesis ATP + acetate + CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.13 Giardia intestinalis Q9Y1N2
-
-
6.2.1.13 Pyrococcus furiosus Q9Y8L0 subunit beta; isoenzyme ACS I
-
6.2.1.13 Pyrococcus furiosus Q9Y8L1 subunit alpha; isoenzyme ACS I
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.13 about 10fold, 15 min at 80ºC and subsequent anion-exchange chromatography Pyrococcus furiosus
6.2.1.13 ion-exchange chromatography Giardia intestinalis

Renatured (Commentary)

EC Number Renatured (Comment) Organism
6.2.1.13 equal amounts of Escherichia coli extracts containing alpha and beta subunits separately expressed mixed and incubated on ice Pyrococcus furiosus
6.2.1.13 equal amounts of Escherichia coli extracts containing alpha and beta subunits separately expressed, mixed and incubated on ice Pyrococcus furiosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.13 cell culture
-
 Pyrococcus furiosus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.13 ADP + phosphate + acetyl-CoA
-
 Pyrococcus furiosus ATP + acetate + CoA
-
 r
6.2.1.13 ADP + phosphate + acetyl-CoA no activity with butanoyl-CoA, propanoyl-CoA and pentanoyl-CoA Giardia intestinalis ATP + acetate + CoA
-
 ?
6.2.1.13 ADP + phosphate + acetyl-CoA enzyme catalyzes acetate formation and ATP generation during fermentation of pyruvate to acetate Giardia intestinalis ATP + acetate + CoA
-
 ?
6.2.1.13 ADP + phosphate + acetyl-CoA major energy-conserving reaction during pyruvate and sugar conversion to acetate, catalyzing acetate formation and ATP synthesis Pyrococcus furiosus ATP + acetate + CoA
-
 r
6.2.1.13 ADP + phosphate + butanoyl-CoA 23% of the activity relative to acetyl-CoA Giardia intestinalis ATP + butanoate + CoA
-
 ?
6.2.1.13 ADP + phosphate + propanoyl-CoA 45% of the activity relative to acetyl-CoA Giardia intestinalis ATP + propanoate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.13 heterotetramer alpha2,beta2, 2 * 47000 (alpha) + 2 * 25000 (beta), SDS-PAGE Pyrococcus furiosus
6.2.1.13 monomer
-
 Giardia intestinalis

Synonyms

EC Number Synonyms Comment Organism
6.2.1.13 acetyl-coenzyme A synthetase (ADP-forming)
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.13 87
-
-
 Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.2.1.13 additional information
-
 80-110ºC, no lose activity upon incubation for 3h at 90ºC, lose about 60% after 2h at 100ºC Pyrococcus furiosus