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Literature summary extracted from

  • Gustin, J.K.; Kessler, E.; Ohman, D.E.
    A substitution at His-120 in the LasA protease of Pseudomonas aeruginosa blocks enzymatic activity without affecting propeptide processing or extracellular secretion (1996), J. Bacteriol., 178, 6608-6617.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.B16 additional information elastase enhances staphylolytic activity of LasA Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B16 DNA sequence determination and analysis, overexpression of gene lasA in Escherichia coli strain JM109 in form of the pre-proLasA precursor protein Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.B16 H120A oligonucleotide-directed mutagenesis, formation of the lasA5 allele, processing to a 20 kDa LasA-H120A protein and secretion to the medium occurs, but the mutant enzyme has no staphylolytic activity Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B16 Trypsin cleaves the enzyme Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.B16 extracellular active processed 20 kDa enzyme, member of the beta-lytic endopeptidase family of extracellular bacterial proteases Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.B16 20000
-
 x * 20000, LasA, SDS-PAGE, x * 42000, proLasA, SDS-PAGE, x * 45582, pre-proLasA, DNA sequence determination Pseudomonas aeruginosa
3.4.24.B16 42000
-
 x * 20000, LasA, SDS-PAGE, x * 42000, proLasA, SDS-PAGE, x * 45582, pre-proLasA, DNA sequence determination Pseudomonas aeruginosa
3.4.24.B16 45582
-
 x * 20000, LasA, SDS-PAGE, x * 42000, proLasA, SDS-PAGE, x * 45582, pre-proLasA, DNA sequence determination Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B16 Elastin + H2O Pseudomonas aeruginosa contributes to the pathogenesis of Pseudomonas aeruginosa ?
-
 ?
3.4.24.B16 Elastin + H2O Pseudomonas aeruginosa FRD1 contributes to the pathogenesis of Pseudomonas aeruginosa ?
-
 ?
3.4.24.B16 additional information Pseudomonas aeruginosa enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells ?
-
 ?
3.4.24.B16 additional information Pseudomonas aeruginosa FRD1 enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells ?
-
 ?
3.4.24.B16 protein + H2O Pseudomonas aeruginosa
-
 peptides
-
 ?
3.4.24.B16 protein + H2O Pseudomonas aeruginosa FRD1
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B16 Pseudomonas aeruginosa
-
-
-
3.4.24.B16 Pseudomonas aeruginosa FRD1
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.B16 proteolytic modification no autoproteolysis, the 31 amino acid residues signal sequence of pre-proLasA is cleaved off by a signal peptidase, processing of the remaining 42 kDa proLasA by incubation with the culture filtrate of Pseudomonas aeruginosa or trypsin to the active 20 kDa LasA Pseudomonas aeruginosa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B16 from the culture supernatant Pseudomonas aeruginosa

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.24.B16 proteolytic degradation of proteins His120 is critical for LasA activity Pseudomonas aeruginosa
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B16 Elastin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.B16 Elastin + H2O contributes to the pathogenesis of Pseudomonas aeruginosa Pseudomonas aeruginosa ?
-
 ?
3.4.24.B16 Elastin + H2O
-
 Pseudomonas aeruginosa FRD1 ?
-
 ?
3.4.24.B16 Elastin + H2O contributes to the pathogenesis of Pseudomonas aeruginosa Pseudomonas aeruginosa FRD1 ?
-
 ?
3.4.24.B16 additional information enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells Pseudomonas aeruginosa ?
-
 ?
3.4.24.B16 additional information enzyme specifically promotes growth inhibition and lysis of Staphylococcus aureus cells Pseudomonas aeruginosa FRD1 ?
-
 ?
3.4.24.B16 protein + H2O
-
 Pseudomonas aeruginosa peptides
-
 ?
3.4.24.B16 protein + H2O
-
 Pseudomonas aeruginosa FRD1 peptides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.B16 ? x * 20000, LasA, SDS-PAGE, x * 42000, proLasA, SDS-PAGE, x * 45582, pre-proLasA, DNA sequence determination Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B16 LasA
-
 Pseudomonas aeruginosa
3.4.24.B16 LasA protease
-
 Pseudomonas aeruginosa
3.4.24.B16 M23.002 Merops-ID Pseudomonas aeruginosa
3.4.24.B16 staphylolysin
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.B16 8.5
-
 assay at Pseudomonas aeruginosa