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Literature summary extracted from

  • Greiner, R.
    Purification and characterization of three phytases from germinated lupine seeds (Lupinus albus var. amiga) (2002), J. Agric. Food Chem., 50, 6858-6864.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Co2+ 0.1 mM-1 mM, slight inhibition Lupinus albus
3.1.3.8 Cu2+ 0.1 mM-1 mM, strong inhibition Lupinus albus
3.1.3.8 F-
-
 Lupinus albus
3.1.3.8 Fe2+ 0.1 mM-1 mM, strong inhibition Lupinus albus
3.1.3.8 Fe3+ 0.1 mM-1 mM, strong inhibition Lupinus albus
3.1.3.8 Mn2+ 0.1 mM-1 mM, slight inhibition Lupinus albus
3.1.3.8 molybdate
-
 Lupinus albus
3.1.3.8 additional information Mg2+ and Ca2+ show no effect at 0.1 mM-1 mM Lupinus albus
3.1.3.8 phosphate
-
 Lupinus albus
3.1.3.8 vanadate
-
 Lupinus albus
3.1.3.8 wolframate
-
 Lupinus albus
3.1.3.8 Zn2+ 0.1 mM-1 mM, strong inhibition Lupinus albus
3.1.3.26 Co2+ 0.1 mM-1 mM, slight inhibition Lupinus albus
3.1.3.26 Cu2+ 0.1 mM-1 mM, strong inhibition Lupinus albus
3.1.3.26 F-
-
 Lupinus albus
3.1.3.26 Fe2+ 0.1 mM-1 mM, strong inhibition Lupinus albus
3.1.3.26 Fe3+ 0.1 mM-1 mM, strong inhibition Lupinus albus
3.1.3.26 Mn2+ 0.1 mM-1 mM, slight inhibition Lupinus albus
3.1.3.26 molybdate
-
 Lupinus albus
3.1.3.26 additional information Mg2+ and Ca2+ show no effect at 0.1 mM-1 mM Lupinus albus
3.1.3.26 phosphate
-
 Lupinus albus
3.1.3.26 vanadate
-
 Lupinus albus
3.1.3.26 wolframate
-
 Lupinus albus
3.1.3.26 Zn2+ 0.1 mM-1 mM, strong inhibition Lupinus albus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.08
-
 myo-inositol hexakisphosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.8 0.123
-
 4-nitrophenyl phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.8 0.13
-
 myo-inositol hexakisphosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.8 0.152
-
 4-nitrophenyl phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.8 0.176
-
 4-nitrophenyl phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.8 0.217
-
 GTP pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.8 0.229
-
 fructose 1,6-diphosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.8 0.3
-
 myo-inositol hexakisphosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.8 0.302
-
 D-glucose 6-phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.8 0.315
-
 AMP pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.8 0.319
-
 AMP pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.8 0.359
-
 D-fructose 1,6-diphosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.8 0.365
-
 AMP pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.8 0.398
-
 GTP pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.8 0.398
-
 ADP pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.8 0.401
-
 D-glucose 6-phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.8 0.403
-
 ADP pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.8 0.412
-
 ATP pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.8 0.423
-
 GTP pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.8 0.445
-
 diphosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.8 0.451
-
 ADP pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.8 0.478
-
 D-fructose 6-phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.8 0.49 2 1-naphthyl phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.8 0.512
-
 2-naphthyl phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.8 0.516
-
 1-naphthyl phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.8 0.531
-
 ATP pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.8 0.531
-
 D-fructose 6-phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.8 0.596
-
 ATP pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.8 0.634
-
 D-glucose 6-phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.8 0.634
-
 2-naphthyl phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.8 0.653
-
 2-naphthyl phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.8 0.667
-
 2-glycerophosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.8 0.676
-
 D-fructose 6-phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.8 0.691
-
 diphosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.8 0.704
-
 2-glycerophosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.8 0.718
-
 1-naphthyl phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.8 0.731
-
 D-fructose 1,6-diphosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.8 0.74
-
 O-phospho-L-serine pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.8 0.776
-
 pyridoxal phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.8 0.8 11 O-phospho-L-serine pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.8 0.812
-
 diphosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.8 0.876
-
 pyridoxal phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.8 0.881
-
 2-glycerophosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.8 0.891
-
 O-phospho-L-serine pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.8 0.915
-
 pyridoxal phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.26 0.08
-
 myo-inositol hexakisphosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.26 0.123
-
 4-nitrophenyl phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.26 0.13
-
 myo-inositol hexakisphosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.26 0.152
-
 4-nitrophenyl phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.26 0.176
-
 4-nitrophenyl phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.26 0.217
-
 GTP pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.26 0.229
-
 D-fructose 1,6-diphosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.26 0.3
-
 myo-inositol hexakisphosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.26 0.302
-
 D-glucose 6-phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.26 0.315
-
 AMP pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.26 0.319
-
 AMP pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.26 0.359
-
 D-fructose 1,6-diphosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.26 0.365
-
 AMP pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.26 0.398
-
 GTP pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.26 0.398
-
 ADP pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.26 0.401
-
 D-glucose 6-phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.26 0.403
-
 ADP pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.26 0.412
-
 ATP pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.26 0.423
-
 GTP pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.26 0.445
-
 diphosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.26 0.451
-
 ADP pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.26 0.478
-
 D-fructose 6-phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.26 0.49 2 1-naphthyl phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.26 0.512
-
 2-naphthyl phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.26 0.516
-
 1-naphthyl phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.26 0.531
-
 ATP pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.26 0.531
-
 D-fructose 6-phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.26 0.596
-
 ATP pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.26 0.634
-
 D-glucose 6-phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.26 0.634
-
 2-naphthyl phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.26 0.653
-
 2-naphthyl phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.26 0.667
-
 2-glycerophosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.26 0.676
-
 D-fructose 6-phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.26 0.691
-
 diphosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.26 0.704
-
 2-glycerophosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.26 0.718
-
 1-naphthyl phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.26 0.731
-
 D-fructose 1,6-diphosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.26 0.74
-
 O-phospho-L-serine pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.26 0.776
-
 pyridoxal phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.26 0.8 11 O-phospho-L-serine pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.26 0.812
-
 diphosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
3.1.3.26 0.876
-
 pyridoxal phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.26 0.881
-
 2-glycerophosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
3.1.3.26 0.891
-
 O-phospho-L-serine pH 5.0, 35°C, phytase LP11 Lupinus albus
3.1.3.26 0.915
-
 pyridoxal phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 additional information phytase LP11, LP12 and LP2 are not metalloenzymes Lupinus albus
3.1.3.26 additional information phytase LP11, LP12 and LP2 are not metalloenzymes Lupinus albus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 56000
-
 phytase LP11 and LP12, gel filtration Lupinus albus
3.1.3.8 57000
-
 1 * 57000, phytase LP11 and LP12, SDS-PAGE Lupinus albus
3.1.3.8 64000
-
 1 * 64000, phytase LP2, SDS-PAGE Lupinus albus
3.1.3.8 65000
-
 phytase LP2, gel filtration Lupinus albus
3.1.3.26 56000
-
 phytase LP11 and LP12, gel filtration Lupinus albus
3.1.3.26 57000
-
 1 * 57000, phytase LP11 and LP12, SDS-PAGE Lupinus albus
3.1.3.26 64000
-
 1 * 64000, phytase LP2, SDS-PAGE Lupinus albus
3.1.3.26 65000
-
 phytase LP2, gel filtration Lupinus albus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Lupinus albus
-
 var. amiga. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Lupinus albus
-
 var. amiga. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 three phytases LP11, LP12 and LP2 Lupinus albus
3.1.3.26 three phytases LP11, LP12 and LP2 Lupinus albus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.8 seedling 4-day-old Lupinus albus
-
3.1.3.26 seedling 4-day-old Lupinus albus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 498
-
 phytase LP2 Lupinus albus
3.1.3.8 539
-
 phytase LP11 Lupinus albus
3.1.3.8 607
-
 phytase LP12 Lupinus albus
3.1.3.26 498
-
 phytase LP2 Lupinus albus
3.1.3.26 539
-
 phytase LP11 Lupinus albus
3.1.3.26 607
-
 phytase LP12 Lupinus albus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 1-naphthyl phosphate + H2O
-
 Lupinus albus 1-naphthol + phosphate
-
 ?
3.1.3.8 2-glycerophosphate + H2O
-
 Lupinus albus glycerate + phosphate
-
 ?
3.1.3.8 2-naphthyl phosphate + H2O
-
 Lupinus albus 2-naphthol + phosphate
-
 ?
3.1.3.8 4-nitrophenyl phosphate + H2O
-
 Lupinus albus 4-nitrophenol + phosphate
-
 ?
3.1.3.8 ADP + H2O
-
 Lupinus albus ?
-
 ?
3.1.3.8 AMP + H2O
-
 Lupinus albus adenosine + phosphate
-
 ?
3.1.3.8 ATP + H2O
-
 Lupinus albus ?
-
 ?
3.1.3.8 D-fructose 1,6-diphosphate + H2O
-
 Lupinus albus ?
-
 ?
3.1.3.8 D-fructose 6-phosphate + H2O
-
 Lupinus albus fructose + phosphate
-
 ?
3.1.3.8 D-glucose 6-phosphate + H2O
-
 Lupinus albus D-glucose + phosphate
-
 ?
3.1.3.8 diphosphate + H2O
-
 Lupinus albus 2 phosphate
-
 ?
3.1.3.8 GTP + H2O
-
 Lupinus albus ?
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O hydrolyzes phytate in a stepwise manner Lupinus albus ? + phosphate
-
 ?
3.1.3.8 O-phospho-L-serine + H2O
-
 Lupinus albus L-serine + phosphate
-
 ?
3.1.3.8 pyridoxal phosphate + H2O
-
 Lupinus albus pyridoxal + phosphate
-
 ?
3.1.3.26 1-naphthyl phosphate + H2O
-
 Lupinus albus 1-naphthol + phosphate
-
 ?
3.1.3.26 2-glycerophosphate + H2O
-
 Lupinus albus glycerate + phosphate
-
 ?
3.1.3.26 2-naphthyl phosphate + H2O
-
 Lupinus albus 2-naphthol + phosphate
-
 ?
3.1.3.26 4-nitrophenyl phosphate + H2O
-
 Lupinus albus 4-nitrophenol + phosphate
-
 ?
3.1.3.26 ADP + H2O
-
 Lupinus albus ?
-
 ?
3.1.3.26 AMP + H2O
-
 Lupinus albus adenosine + phosphate
-
 ?
3.1.3.26 ATP + H2O
-
 Lupinus albus ?
-
 ?
3.1.3.26 D-fructose 1,6-diphosphate + H2O
-
 Lupinus albus ?
-
 ?
3.1.3.26 D-fructose 6-phosphate + H2O
-
 Lupinus albus D-fructose + phosphate
-
 ?
3.1.3.26 D-glucose 6-phosphate + H2O
-
 Lupinus albus D-glucose + phosphate
-
 ?
3.1.3.26 diphosphate + H2O
-
 Lupinus albus 2 phosphate
-
 ?
3.1.3.26 GTP + H2O
-
 Lupinus albus ?
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O hydrolyzes phytate in a stepwise manner Lupinus albus ? + phosphate
-
 ?
3.1.3.26 O-phospho-L-serine + H2O
-
 Lupinus albus L-serine + phosphate
-
 ?
3.1.3.26 pyridoxal phosphate + H2O
-
 Lupinus albus pyridoxal + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 monomer 1 * 57000, phytase LP11 and LP12, SDS-PAGE Lupinus albus
3.1.3.8 monomer 1 * 64000, phytase LP2, SDS-PAGE Lupinus albus
3.1.3.26 monomer 1 * 57000, phytase LP11 and LP12, SDS-PAGE Lupinus albus
3.1.3.26 monomer 1 * 64000, phytase LP2, SDS-PAGE Lupinus albus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 50
-
 phytase LP11, LP12 and LP2 Lupinus albus
3.1.3.26 50
-
 phytase LP11, LP12 and LP2 Lupinus albus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 45
-
 90 min, no significant loss of activity at temperatures up ro, phytase LP11, LP12 and LP2 Lupinus albus
3.1.3.8 50
-
 90 min, 15% loss of activity of phytase, phytase LP11, LP12 and LP2 Lupinus albus
3.1.3.8 60
-
 90 min, 90 min, 79% loss of activity of phytase LP22, 92% loss of activity of phytase LP12 and 85% loss of activity of phytase LP2 Lupinus albus
3.1.3.26 45
-
 90 min, no significant loss of activity at temperatures up ro, phytase LP11, LP12 and LP2 Lupinus albus
3.1.3.26 50
-
 90 min, 15% loss of activity of phytase, phytase LP11, LP12 and LP2 Lupinus albus
3.1.3.26 60
-
 90 min, 90 min, 79% loss of activity of phytase LP22, 92% loss of activity of phytase LP12 and 85% loss of activity of phytase LP2 Lupinus albus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 additional information
-
 additional information
-
 Lupinus albus
3.1.3.26 additional information
-
 additional information
-
 Lupinus albus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
 phytase LP11, LP12 and LP2 Lupinus albus
3.1.3.26 5
-
 phytase LP11, LP12 and LP2 Lupinus albus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 3 8 enzyme is virtually inactive below pH 3.0 and above pH 8.0, phytase LP11, LP12 and LP2 Lupinus albus
3.1.3.26 3 8 enzyme is virtually inactive below pH 3.0 and above pH 8.0, phytase LP11, LP12 and LP2 Lupinus albus