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Literature summary extracted from

  • Kamita, S.G.; Hinton, A.C.; Wheelock, C.E.; Wogulis, M.D.; Wilson, D.K.; Wolf, N.M.; Stok, J.E.; Hock, B.; Hammock, B.D.
    Juvenile hormone (JH) esterase: why are you so JH specific? (2003), Insect Biochem. Mol. Biol., 33, 1261-1273.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.59 acetone activates even at higher concentrations Manduca sexta
3.1.1.59 acetone at low concentrations, slight activation Trichoplusia ni
3.1.1.59 acetone at low concentrations, slight activation Heliothis virescens
3.1.1.59 ethanol activates even at higher concentrations Manduca sexta
3.1.1.59 Isopropanol activates even at higher concentrations Manduca sexta
3.1.1.59 Triton X-100 activates at low concentrations Manduca sexta
3.1.1.59 Triton X-100 at low concentrations, slight activation Trichoplusia ni
3.1.1.59 Triton X-100 at low concentrations, slight activation Heliothis virescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.59 expression in High 5 cells Tenebrio molitor
3.1.1.59 expression in High 5 cells Manduca sexta
3.1.1.59 expression in High 5 cells Heliothis virescens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.59 recombinant JHE, complexed with 1,1,1-trifluoro-3-octylsulfanyl-propan-2-one Manduca sexta

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.59 1,1,1-trifluoro-3-decylsulfanyl-propan-2-one IC50: 0.99 nM, juvenile hormone III hydrolysis Heliothis virescens
3.1.1.59 1,1,1-trifluoro-3-decylsulfanyl-propan-2-one IC50: 2.8 nM, juvenile hormone III hydrolysis Manduca sexta
3.1.1.59 1,1,1-trifluoro-3-decylsulfanyl-propan-2-one IC50: 3.6 nM, juvenile hormone III hydrolysis Tenebrio molitor
3.1.1.59 1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-one IC50: 2.2 nM, juvenile hormone III hydrolysis Heliothis virescens
3.1.1.59 1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-one IC50: 4.4 nM, juvenile hormone III hydrolysis Manduca sexta
3.1.1.59 1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-one IC50: 12 nM, juvenile hormone III hydrolysis Tenebrio molitor
3.1.1.59 1,1,1-trifluoro-3-hexylsulfanyl-propan-2-one IC50: 1100 nM, juvenile hormone III hydrolysis Heliothis virescens
3.1.1.59 1,1,1-trifluoro-3-hexylsulfanyl-propan-2-one IC50: 1700 nM, juvenile hormone III hydrolysis Manduca sexta
3.1.1.59 1,1,1-trifluoro-3-hexylsulfanyl-propan-2-one IC50: 14 nM, juvenile hormone III hydrolysis Tenebrio molitor
3.1.1.59 1,1,1-trifluoro-3-octylsulfanyl-propan-2-one IC50: 5.8 nM, juvenile hormone III hydrolysis Heliothis virescens
3.1.1.59 1,1,1-trifluoro-3-octylsulfanyl-propan-2-one IC50: 9.1 nM, juvenile hormone III hydrolysis Manduca sexta
3.1.1.59 1,1,1-trifluoro-3-octylsulfanyl-propan-2-one IC50: 4.6 nM, juvenile hormone III hydrolysis Tenebrio molitor
3.1.1.59 1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-one IC50: 75 nM, juvenile hormone III hydrolysis Heliothis virescens
3.1.1.59 1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-one IC50: 400 nM, juvenile hormone III hydrolysis Manduca sexta
3.1.1.59 1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-one IC50: 36 nM, juvenile hormone III hydrolysis Tenebrio molitor
3.1.1.59 1-octyl (1-(3,3,3-trifluoropropan-2,2-dihydroxy))sulfone potent, specific inhibitor Trichoplusia ni
3.1.1.59 trifluoromethylketone with chain lengths of 8, 10 or 12 carbons, highly effective inhibitors Trichoplusia ni
3.1.1.59 Triton X-100 inhibits at low concentrations Tenebrio molitor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.59 juvenile hormone + H2O Tenebrio molitor
-
 Juvenile hormone acid + methanol
-
 ?
3.1.1.59 juvenile hormone + H2O Manduca sexta
-
 Juvenile hormone acid + methanol
-
 ?
3.1.1.59 juvenile hormone + H2O Trichoplusia ni
-
 Juvenile hormone acid + methanol
-
 ?
3.1.1.59 juvenile hormone + H2O Heliothis virescens
-
 Juvenile hormone acid + methanol
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.59 Acetone stable in Trichoplusia ni
3.1.1.59 Acetone stable in, activates even at higher concentrations Manduca sexta
3.1.1.59 Acetone stable, even at higher concentrations Heliothis virescens
3.1.1.59 Acetone unstable, rapid decrease in activity at low concentrations Tenebrio molitor
3.1.1.59 Ethanol stable in, activates even at higher concentrations Manduca sexta
3.1.1.59 Ethanol stable, even at higher concentrations Heliothis virescens
3.1.1.59 Ethanol unstable, rapid decrease in activity at low concentrations Tenebrio molitor
3.1.1.59 isopropanol stable in, activates even at higher concentrations Manduca sexta
3.1.1.59 isopropanol stable, even at higher concentrations Heliothis virescens
3.1.1.59 isopropanol unstable, rapid decrease in activity at low concentrations Tenebrio molitor
3.1.1.59 additional information remarkably resistant to the addition of organic co-solvents and detergent Manduca sexta
3.1.1.59 Triton X-100 stable in Trichoplusia ni
3.1.1.59 Triton X-100 stable in, activates at low concentrations Manduca sexta
3.1.1.59 Triton X-100 stable, even at higher concentrations Heliothis virescens
3.1.1.59 Triton X-100 unstable, rapid decrease in activity at low concentrations Tenebrio molitor

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.59 Heliothis virescens
-
-
-
3.1.1.59 Manduca sexta
-
-
-
3.1.1.59 Tenebrio molitor
-
-
-
3.1.1.59 Trichoplusia ni
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.59 glycoprotein JHE is highly glycosylated Heliothis virescens
3.1.1.59 no glycoprotein
-
 Manduca sexta

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.59 recombinant JHE Tenebrio molitor
3.1.1.59 recombinant JHE Manduca sexta
3.1.1.59 recombinant JHE Heliothis virescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.59 hemolymph of larvae at second day of the fifth instar Trichoplusia ni
-
3.1.1.59 larva second day of the fifth instar, hemolymph Trichoplusia ni
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.59 additional information
-
 specific activities for several alpha-naphthyl and p-nitrophenyl substrates Tenebrio molitor
3.1.1.59 additional information
-
 specific activities for several alpha-naphthyl and p-nitrophenyl substrates Manduca sexta
3.1.1.59 additional information
-
 specific activities for several alpha-naphthyl and p-nitrophenyl substrates Heliothis virescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.59 alpha-naphthyl acetate + H2O
-
 Trichoplusia ni alpha-naphthol + acetate
-
 ?
3.1.1.59 alpha-naphthyl acetate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from acetate to octanoate Manduca sexta alpha-naphthol + acetate
-
 ?
3.1.1.59 alpha-naphthyl acetate + H2O lower activity than with alpha-naphthyl propionate Tenebrio molitor alpha-naphthol + acetate
-
 ?
3.1.1.59 alpha-naphthyl acetate + H2O lower activity than with alpha-naphthyl propionate Heliothis virescens alpha-naphthol + acetate
-
 ?
3.1.1.59 alpha-naphthyl butyrate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from acetate to octanoate Manduca sexta alpha-naphthol + butyrate
-
 ?
3.1.1.59 alpha-naphthyl butyrate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate Tenebrio molitor alpha-naphthol + butyrate
-
 ?
3.1.1.59 alpha-naphthyl butyrate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate Heliothis virescens alpha-naphthol + butyrate
-
 ?
3.1.1.59 alpha-naphthyl hexanoate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from acetate to octanoate Manduca sexta alpha-naphthol + hexanoate
-
 ?
3.1.1.59 alpha-naphthyl hexanoate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate Tenebrio molitor alpha-naphthol + hexanoate
-
 ?
3.1.1.59 alpha-naphthyl hexanoate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate Heliothis virescens alpha-naphthol + hexanoate
-
 ?
3.1.1.59 alpha-naphthyl octanoate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from acetate to octanoate Manduca sexta alpha-naphthol + octanoate
-
 ?
3.1.1.59 alpha-naphthyl octanoate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate Tenebrio molitor alpha-naphthol + octanoate
-
 ?
3.1.1.59 alpha-naphthyl octanoate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate Heliothis virescens alpha-naphthol + octanoate
-
 ?
3.1.1.59 alpha-naphthyl propionate + H2O activity decreases with increasing the chain length of the alpha-naphthyl substrates from acetate to octanoate Manduca sexta alpha-naphthol + propionate
-
 ?
3.1.1.59 alpha-naphthyl propionate + H2O higher activity than with alpha-naphthyl acetate, activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate Tenebrio molitor alpha-naphthol + propionate
-
 ?
3.1.1.59 alpha-naphthyl propionate + H2O higher activity than with alpha-naphthyl acetate, activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate Heliothis virescens alpha-naphthol + propionate
-
 ?
3.1.1.59 juvenile hormone + H2O
-
 Tenebrio molitor Juvenile hormone acid + methanol
-
 ?
3.1.1.59 juvenile hormone + H2O
-
 Manduca sexta Juvenile hormone acid + methanol
-
 ?
3.1.1.59 juvenile hormone + H2O
-
 Trichoplusia ni Juvenile hormone acid + methanol
-
 ?
3.1.1.59 juvenile hormone + H2O
-
 Heliothis virescens Juvenile hormone acid + methanol
-
 ?
3.1.1.59 juvenile hormone I + H2O
-
 Tenebrio molitor juvenile hormone I acid + methanol
-
 ?
3.1.1.59 juvenile hormone I + H2O
-
 Manduca sexta juvenile hormone I acid + methanol
-
 ?
3.1.1.59 juvenile hormone I + H2O
-
 Heliothis virescens juvenile hormone I acid + methanol
-
 ?
3.1.1.59 juvenile hormone III + H2O
-
 Tenebrio molitor juvenile hormone III acid + methanol
-
 ?
3.1.1.59 juvenile hormone III + H2O
-
 Manduca sexta juvenile hormone III acid + methanol
-
 ?
3.1.1.59 juvenile hormone III + H2O
-
 Trichoplusia ni juvenile hormone III acid + methanol
-
 ?
3.1.1.59 juvenile hormone III + H2O
-
 Heliothis virescens juvenile hormone III acid + methanol
-
 ?
3.1.1.59 p-nitrophenyl acetate + H2O
-
 Trichoplusia ni p-nitrophenol + acetate
-
 ?
3.1.1.59 p-nitrophenyl acetate + H2O higher specific activity than for p-nitrophenyl valerate Tenebrio molitor p-nitrophenol + acetate
-
 ?
3.1.1.59 p-nitrophenyl acetate + H2O higher specific activity than for p-nitrophenyl valerate Manduca sexta p-nitrophenol + acetate
-
 ?
3.1.1.59 p-nitrophenyl acetate + H2O higher specific activity than for p-nitrophenyl valerate Heliothis virescens p-nitrophenol + acetate
-
 ?
3.1.1.59 p-nitrophenyl valerate + H2O lower specific activity than for p-nitrophenyl acetate Tenebrio molitor p-nitrophenol + valerate
-
 ?
3.1.1.59 p-nitrophenyl valerate + H2O lower specific activity than for p-nitrophenyl acetate Manduca sexta p-nitrophenol + valerate
-
 ?
3.1.1.59 p-nitrophenyl valerate + H2O lower specific activity than for p-nitrophenyl acetate Heliothis virescens p-nitrophenol + valerate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.59 JHE
-
 Tenebrio molitor
3.1.1.59 JHE
-
 Manduca sexta
3.1.1.59 JHE
-
 Trichoplusia ni
3.1.1.59 JHE
-
 Heliothis virescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.59 30
-
 assay at Tenebrio molitor
3.1.1.59 30
-
 assay at Manduca sexta
3.1.1.59 30
-
 assay at Trichoplusia ni
3.1.1.59 30
-
 assay at Heliothis virescens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.59 0.00000099
-
 IC50: 0.99 nM, juvenile hormone III hydrolysis Heliothis virescens 1,1,1-trifluoro-3-decylsulfanyl-propan-2-one
3.1.1.59 0.0000022
-
 IC50: 2.2 nM, juvenile hormone III hydrolysis Heliothis virescens 1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-one
3.1.1.59 0.0000028
-
 IC50: 2.8 nM, juvenile hormone III hydrolysis Manduca sexta 1,1,1-trifluoro-3-decylsulfanyl-propan-2-one
3.1.1.59 0.0000036
-
 IC50: 3.6 nM, juvenile hormone III hydrolysis Tenebrio molitor 1,1,1-trifluoro-3-decylsulfanyl-propan-2-one
3.1.1.59 0.0000044
-
 IC50: 4.4 nM, juvenile hormone III hydrolysis Manduca sexta 1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-one
3.1.1.59 0.0000046
-
 IC50: 4.6 nM, juvenile hormone III hydrolysis Tenebrio molitor 1,1,1-trifluoro-3-octylsulfanyl-propan-2-one
3.1.1.59 0.0000058
-
 IC50: 5.8 nM, juvenile hormone III hydrolysis Heliothis virescens 1,1,1-trifluoro-3-octylsulfanyl-propan-2-one
3.1.1.59 0.0000091
-
 IC50: 9.1 nM, juvenile hormone III hydrolysis Manduca sexta 1,1,1-trifluoro-3-octylsulfanyl-propan-2-one
3.1.1.59 0.000012
-
 IC50: 12 nM, juvenile hormone III hydrolysis Tenebrio molitor 1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-one
3.1.1.59 0.000014
-
 IC50: 14 nM, juvenile hormone III hydrolysis Tenebrio molitor 1,1,1-trifluoro-3-hexylsulfanyl-propan-2-one
3.1.1.59 0.000036
-
 IC50: 36 nM, juvenile hormone III hydrolysis Tenebrio molitor 1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-one
3.1.1.59 0.000075
-
 IC50: 75 nM, juvenile hormone III hydrolysis Heliothis virescens 1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-one
3.1.1.59 0.0004
-
 IC50: 400 nM, juvenile hormone III hydrolysis Manduca sexta 1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-one
3.1.1.59 0.0011
-
 IC50: 1100 nM, juvenile hormone III hydrolysis Heliothis virescens 1,1,1-trifluoro-3-hexylsulfanyl-propan-2-one
3.1.1.59 0.0017
-
 IC50: 1700 nM, juvenile hormone III hydrolysis Manduca sexta 1,1,1-trifluoro-3-hexylsulfanyl-propan-2-one