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Literature summary extracted from

  • Schenk, G.; Korsinczky, M.L.J.; Hume, D.A.; Hamilton, S.; DeJersey, J.
    Purple acid phosphatases from bacteria: similarities to mammalian and plant enzymes (2000), Gene, 255, 419-424.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 CuSO4 10 mM, 88% inhibition Solanum lycopersicum
3.1.3.2 F-
-
 Solanum lycopersicum
3.1.3.2 FeCl3 10 mM, complete inhibition Solanum lycopersicum
3.1.3.2 molybdate
-
 Solanum lycopersicum
3.1.3.2 phosphate
-
 Solanum lycopersicum
3.1.3.2 vanadate
-
 Solanum lycopersicum
3.1.3.2 ZnCl2 10 mM, 99% inhibition Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.16
-
 GTP pH 5.1, 25°C Solanum lycopersicum
3.1.3.2 0.18
-
 2-naphthyl phosphate pH 5.1, 25°C Solanum lycopersicum
3.1.3.2 0.22
-
 phosphoenolpyruvate pH 5.1, 25°C Solanum lycopersicum
3.1.3.2 0.23
-
 IDP pH 5.1, 25°C Solanum lycopersicum
3.1.3.2 0.5
-
 ATP pH 5.1, 25°C Solanum lycopersicum
3.1.3.2 1.4
-
 ADP pH 5.1, 25°C Solanum lycopersicum
3.1.3.2 1.4
-
 bis-(p-nitrophenyl)phosphate pH 5.1, 25°C Solanum lycopersicum
3.1.3.2 2.5
-
 UTP pH 5.1, 25°C Solanum lycopersicum
3.1.3.2 2.7
-
 pyridoxal 5'-phosphate
-
 Solanum lycopersicum
3.1.3.2 3.4
-
 diphosphate pH 5.1, 25°C Solanum lycopersicum
3.1.3.2 3.4
-
 1-naphthyl phosphate pH 5.1, 25°C Solanum lycopersicum
3.1.3.2 3.6
-
 GTP pH 5.1, 25°C Solanum lycopersicum
3.1.3.2 4.1
-
 phosphotyrosine pH 5.1, 25°C Solanum lycopersicum
3.1.3.2 7.1
-
 Phenyl phosphate pH 5.1, 25°C Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.2 intracellular
-
 Solanum lycopersicum 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 MgCl2 10 mM, 150% activated Solanum lycopersicum
3.1.3.2 MnCl2 10 mM, 130% activated Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 57000
-
 alphabeta, 1 * 63000 + 1 * 57000, SDS-PAGE Solanum lycopersicum
3.1.3.2 63000
-
 alphabeta, 1 * 63000 + 1 * 57000, SDS-PAGE Solanum lycopersicum
3.1.3.2 142000
-
 non-denaturing PAGE, gel filtration Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Solanum lycopersicum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.2 glycoprotein
-
 Solanum lycopersicum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
 Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 cell suspension culture
-
 Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 620
-
-
 Solanum lycopersicum

Storage Stability

EC Number Storage Stability Organism
3.1.3.2 -80°C, purified enzyme is stable for at least 4 months Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-naphthyl phosphate + H2O
-
 Solanum lycopersicum 1-naphthol + phosphate
-
 ?
3.1.3.2 2-naphthyl phosphate + H2O
-
 Solanum lycopersicum 2-naphthol + phosphate
-
 ?
3.1.3.2 ADP + H2O
-
 Solanum lycopersicum ?
-
 ?
3.1.3.2 ATP + H2O
-
 Solanum lycopersicum ?
-
 ?
3.1.3.2 bis-(p-nitrophenyl)phosphate + H2O
-
 Solanum lycopersicum ?
-
 ?
3.1.3.2 diphosphate + H2O
-
 Solanum lycopersicum 2 phosphate
-
 ?
3.1.3.2 GDP + H2O
-
 Solanum lycopersicum ?
-
 ?
3.1.3.2 GTP + H2O
-
 Solanum lycopersicum ?
-
 ?
3.1.3.2 IDP + H2O
-
 Solanum lycopersicum ?
-
 ?
3.1.3.2 additional information enzyme also exhibits significant peroxidase activity, which is optimal at pH 9.0 and insensitive to Mg2+ or molybdate Solanum lycopersicum ?
-
 ?
3.1.3.2 phenyl phosphate + H2O
-
 Solanum lycopersicum phenol + phosphate
-
 ?
3.1.3.2 phosphoenolpyruvate + H2O
-
 Solanum lycopersicum pyruvate + phosphate
-
 ?
3.1.3.2 phosphotyrosine + H2O
-
 Solanum lycopersicum ?
-
 ?
3.1.3.2 pyridoxal 5'-phosphate + H2O
-
 Solanum lycopersicum pyridoxal + phosphate
-
 ?
3.1.3.2 UTP + H2O
-
 Solanum lycopersicum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 dimer alphabeta, 1 * 63000 + 1 * 57000, SDS-PAGE Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 IAP
-
 Solanum lycopersicum
3.1.3.2 intracellular acid phosphatase
-
 Solanum lycopersicum
3.1.3.2 purple acid phosphatase
-
 Solanum lycopersicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 50
-
 5 min, 20% loss of activity Solanum lycopersicum
3.1.3.2 65
-
 5 min, 50% loss of activity Solanum lycopersicum
3.1.3.2 70
-
 5 min, 80% loss of activity Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.1
-
-
 Solanum lycopersicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.2 0.0002
-
 molybdate pH 5.1 Solanum lycopersicum
3.1.3.2 0.0004
-
 molybdate pH 5.7 Solanum lycopersicum
3.1.3.2 0.0005
-
 molybdate pH 6.2 Solanum lycopersicum
3.1.3.2 0.0065
-
 vanadate pH 5.1 Solanum lycopersicum
3.1.3.2 0.013
-
 vanadate pH 5.7 Solanum lycopersicum
3.1.3.2 0.015
-
 vanadate pH 6.2 Solanum lycopersicum
3.1.3.2 0.094
-
 F- pH 5.1 Solanum lycopersicum
3.1.3.2 0.14
-
 phosphate pH 5.7 Solanum lycopersicum
3.1.3.2 0.14
-
 F- pH 5.7 Solanum lycopersicum
3.1.3.2 0.42
-
 phosphate pH 5.7 Solanum lycopersicum
3.1.3.2 0.85
-
 F- pH 6.2 Solanum lycopersicum
3.1.3.2 1.6
-
 phosphate pH 5.1 Solanum lycopersicum