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Literature summary extracted from

  • Kitaguchi, H.; Uchida, A.; Ishida, Y.
    Purification and characterization of L-methionine decarboxylase from Crypthecodinium cohnii (1999), Fish. Sci., 65, 613-617.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.1.1.57 little effect for stabilization to add pyridoxal 5'-phosphate, glycerol or 2-mercaptoethanol Crypthecodinium cohnii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.57 carboxymethoxylamine hemihydrochloride 1 mM, 38.9% activity Crypthecodinium cohnii
4.1.1.57 hydroxylamine 1 mM, 1.6% activity Crypthecodinium cohnii
4.1.1.57 phenylhydrazine 1 mM, 29% activity Crypthecodinium cohnii
4.1.1.57 Semicarbazide hydrochloride 1 mM, 21.6% activity Crypthecodinium cohnii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.57 additional information the enzyme is not membrane bound, because its activity can be solubilized into the 50 mM potassium phosphate buffer pH 7.2 without detergents Crypthecodinium cohnii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.57 100000
-
 2 * 100000, SDS-PAGE Crypthecodinium cohnii
4.1.1.57 204000
-
 gel filtration chromatography Crypthecodinium cohnii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.57 L-methionine Crypthecodinium cohnii
-
 3-methylthiopropanamine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.57 Crypthecodinium cohnii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.57 four purification steps including anion-exchange chromatography and size-exclusion chromatography Crypthecodinium cohnii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.57 0.021
-
 crude extract Crypthecodinium cohnii
4.1.1.57 4.45
-
 after sequential DEAE-Cellulose, Resource-Q, Mono-Q and Superdex 200 chromatography Crypthecodinium cohnii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.57 L-methionine
-
 Crypthecodinium cohnii 3-methylthiopropanamine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.57 homodimer 2 * 100000, SDS-PAGE Crypthecodinium cohnii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.57 30
-
-
 Crypthecodinium cohnii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.57 20 45 retains approximately 70% activity in this range Crypthecodinium cohnii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.57 7.3
-
-
 Crypthecodinium cohnii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.57 6.5 8 potassium phosphate buffer, values between 66% and 85% activity with respect to optimum pH Crypthecodinium cohnii

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.57 pyridoxal 5'-phosphate 0.1 mM: 100% activity, 0.001 mM: 50.4% activity, 0.00001 mM: 30.4% activity. No pyridoxal 5'-phosphate added: 22% activity Crypthecodinium cohnii