Any feedback?
Literature summary extracted from
- Cloning and expression of a pectate lyase from the oral spirochete Treponema pectinovorum ATCC 33768 (2003), FEMS Microbiol. Lett., 226, 385-390.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.2.2 | expression in Escherichia coli | Treponema pectinovorum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.2 | EDTA | 3 mM, complete inhibition. Lyase activity can be restored by further addition of 5 mM CaCl2 | Treponema pectinovorum |  |
| 4.2.2.2 | MnSO4 | 0.1 mM, 10% decrease in activity | Treponema pectinovorum | |
| 4.2.2.2 | ZnSO4 | 0.1 mM, 90% decrease in activity | Treponema pectinovorum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.2 | Ca2+ | requires Ca2+, optimal activity with 0.25 mM | Treponema pectinovorum | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.2 | Treponema pectinovorum | Q7X0P2 | ATCC 33768 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.2.2 | 6*His-tagged recombinant enzyme | Treponema pectinovorum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.2 | esterified pectin | 94% esterified pectin. 97% of the activity with polygalacturonic acid | Treponema pectinovorum | ? | - |
? | |
| 4.2.2.2 | pectin | 80% of the activity with polygalacturonic acid | Treponema pectinovorum | ? | - |
? | |
| 4.2.2.2 | polygalacturonic acid | - |
Treponema pectinovorum | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.2.2 | PelA | - |
Treponema pectinovorum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.2 | 45 | - |
- |
Treponema pectinovorum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.2 | 37 | 50 | 37°C: about 85% of maximal activity, 50°C: about 75% of maximal activity | Treponema pectinovorum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.2 | 9 | - |
- |
Treponema pectinovorum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.2 | 8 | 10 | pH 8.0: about 50% of maximal activity, pH 10.0: about 40% of maximal activity | Treponema pectinovorum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
