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Literature summary extracted from

  • Nolan, L.M.; Harnedy, P.A.; Turner, P.; Hearne, A.B.; O'Reilly, C.
    The cyanide hydratase enzyme of Fusarium lateritium also has nitrilase activity (2003), FEMS Microbiol. Lett., 221, 161-165.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.66 overexpression of the wild-type and mutant enzymes in Escherichia coli XL1-Blue Fusarium lateritium

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.66 D275E site-directed mutagenesis, 0-29% reduced activity compared to the wild-type enzyme Fusarium lateritium
4.2.1.66 F170L site-directed mutagenesis, inactive mutant compared to the wild-type enzyme Fusarium lateritium
4.2.1.66 K136R site-directed mutagenesis, 52% reduced activity compared to the wild-type enzyme Fusarium lateritium
4.2.1.66 M302S site-directed mutagenesis, 22% reduced activity compared to the wild-type enzyme Fusarium lateritium
4.2.1.66 S13A site-directed mutagenesis, 19-47% reduced activity compared to the wild-type enzyme Fusarium lateritium
4.2.1.66 T12P site-directed mutagenesis, inactive mutant Fusarium lateritium
4.2.1.66 T12Q site-directed mutagenesis, 10-48% reduced activity compared to the wild-type enzyme Fusarium lateritium
4.2.1.66 V281A site-directed mutagenesis, 8% reduced activity compared to the wild-type enzyme Fusarium lateritium

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.66 Fusarium lateritium P32963
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.66 formamide = cyanide + H2O Phe170 is an active site residue Fusarium lateritium
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.66 additional information
-
 cyanide hydratase and nitrilase activity Fusarium lateritium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.66 acetonitrile + H2O
-
 Fusarium lateritium ?
-
 ?
4.2.1.66 benzonitrile + H2O best substrate for nitrilase activity Fusarium lateritium ?
-
 ?
4.2.1.66 HCN + H2O
-
 Fusarium lateritium formamide
-
 ?
4.2.1.66 additional information the enzyme also shows low nitrilase activity, which is sufficient for the fungus to grow on acetonitrile, propionitrile, or benzonitrile as sole nitrogen source Fusarium lateritium ?
-
 ?
4.2.1.66 propionitrile + H2O
-
 Fusarium lateritium ?
-
 ?