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Literature summary extracted from

  • Honda, K.; Kataoka, M.; Ono, H.; Sakamoto, K.; Kita, S.; Shimizu, S.
    Purification and characterization of a novel esterase promising for the production of useful compounds from Microbacterium sp. 7-1W (2002), FEMS Microbiol. Lett., 206, 221-227.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.1 synthesis enzyme is useful for deprotection of the compounds in the production of cetraxate and D-panothenate Microbacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 8-hydroxyquinoline
-
Microbacterium sp.
3.1.1.1 diisopropyl phosphofluoridate
-
Microbacterium sp.
3.1.1.1 HgCl2 slight inhibition Microbacterium sp.
3.1.1.1 additional information no inhibition by NaCl, KCl, MgCl2, MnCl2, CoCl2, NiCl2 Microbacterium sp.
3.1.1.1 N-ethylmaleimide slight inhibition Microbacterium sp.
3.1.1.1 PMSF
-
Microbacterium sp.
3.1.1.1 Sodium cyanide poor inhibition Microbacterium sp.
3.1.1.1 ZnCl2 slight inhibition Microbacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.12
-
ethyl cetraxate pH 7.4, 30°C Microbacterium sp.
3.1.1.1 0.17
-
isopropyl cetraxate pH 7.4, 30°C Microbacterium sp.
3.1.1.1 0.38
-
methyl cetraxate pH 7.4, 30°C Microbacterium sp.
3.1.1.1 2.49
-
methyl 3-(4-hydroxyphenyl)propionate pH 7.4, 30°C Microbacterium sp.
3.1.1.1 10
-
n-propyl D-pantothenate pH 7.4, 30°C Microbacterium sp.
3.1.1.1 12.1
-
n-propyl L-pantothenate pH 7.4, 30°C Microbacterium sp.
3.1.1.1 16.7
-
ethyl D-pantothenate pH 7.4, 30°C Microbacterium sp.
3.1.1.1 18.3
-
ethyl L-pantothenate pH 7.4, 30°C Microbacterium sp.
3.1.1.1 29.4
-
methyl D-pantothenate pH 7.4, 30°C Microbacterium sp.
3.1.1.1 105
-
methyl L-pantothenate pH 7.4, 30°C Microbacterium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.1 membrane
-
Microbacterium sp. 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 62000
-
n * 62000, SDS-PAGE Microbacterium sp.
3.1.1.1 600000
-
gel filtration Microbacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Microbacterium sp.
-
AKU 163
-
3.1.1.1 Microbacterium sp. 7-1W
-
AKU 163
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 solubilization with Brij58, 13.8fold to apparent homogeneity Microbacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 26
-
purified enzyme Microbacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 benzyl (trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate) + H2O i.e. benzyl cetraxate Microbacterium sp. benzol + trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate
-
?
3.1.1.1 ethyl (trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate) + H2O i.e. ethyl cetraxate Microbacterium sp. ethanol + trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate
-
?
3.1.1.1 ethyl (trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate) + H2O i.e. ethyl cetraxate Microbacterium sp. 7-1W ethanol + trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate
-
?
3.1.1.1 ethyl acetate + H2O
-
Microbacterium sp. ethanol + acetate
-
?
3.1.1.1 ethyl acetate + H2O
-
Microbacterium sp. 7-1W ethanol + acetate
-
?
3.1.1.1 ethyl butyrate + H2O
-
Microbacterium sp. ethanol + butanoate
-
?
3.1.1.1 ethyl caproate + H2O
-
Microbacterium sp. ethanol + caproate
-
?
3.1.1.1 ethyl cetraxate + H2O
-
Microbacterium sp. ethanol + cetraxate
-
?
3.1.1.1 ethyl D-pantothenate + H2O
-
Microbacterium sp. ethanol + D-pantothenate
-
?
3.1.1.1 ethyl L-pantothenate + H2O
-
Microbacterium sp. ethanol + L-pantothenate
-
?
3.1.1.1 ethyl valerate + H2O
-
Microbacterium sp. ethanol + valerate
-
?
3.1.1.1 isopropyl (trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate) + H2O i.e. propyl cetraxate Microbacterium sp. isopropanol + trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate
-
?
3.1.1.1 isopropyl (trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate) + H2O i.e. propyl cetraxate Microbacterium sp. 7-1W isopropanol + trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate
-
?
3.1.1.1 isopropyl cetraxate + H2O
-
Microbacterium sp. isopropanol + cetraxate
-
?
3.1.1.1 methyl (trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate) + H2O i.e. methyl cetraxate Microbacterium sp. methanol + trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate
-
?
3.1.1.1 methyl (trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate) + H2O i.e. methyl cetraxate Microbacterium sp. 7-1W methanol + trans-4-[[4-(aminomethyl)cyclohexyl]carbonyl]oxybenzen-propionate
-
?
3.1.1.1 methyl 3-(4-hydroxyphenyl)propionate + H2O
-
Microbacterium sp. methanol + 3-(4-hydroxyphenyl)propionate
-
?
3.1.1.1 methyl cetraxate + H2O
-
Microbacterium sp. methanol + cetraxate
-
?
3.1.1.1 methyl D-pantothenate + H2O
-
Microbacterium sp. methanol + D-pantothenate
-
?
3.1.1.1 methyl L-pantothenate + H2O
-
Microbacterium sp. methanol + L-pantothenate
-
?
3.1.1.1 additional information very low activity with methyl or ethyl esters of amino acids Microbacterium sp. ?
-
?
3.1.1.1 additional information very low activity with methyl or ethyl esters of amino acids Microbacterium sp. 7-1W ?
-
?
3.1.1.1 n-propyl D-pantothenate + H2O
-
Microbacterium sp. n-propanol + D-pantothenate
-
?
3.1.1.1 n-propyl L-pantothenate + H2O best substrate Microbacterium sp. n-propanol + L-pantothenate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 oligomer n * 62000, SDS-PAGE Microbacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 30
-
with substrate methyl cetraxate Microbacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 35
-
below, 30 min stable, pH 7.0 Microbacterium sp.
3.1.1.1 40
-
30 min, pH 7.0, 13% remaining activity Microbacterium sp.
3.1.1.1 45
-
30 min, pH 7.0, 9% remaining activity Microbacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7
-
about Microbacterium sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.1 Microbacterium sp. isoelectric focusing
-
4