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Literature summary extracted from
- Characterization of the maltooligosyl trehalose synthase from the thermophilic archaeon Sulfolobus acidocaldarius (2001), FEMS Microbiol. Lett., 194, 201-206.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.15 | overexpression in Escherichia coli using the T7-expression system | Sulfolobus acidocaldarius |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.15 | Sulfolobus acidocaldarius | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.15 | Maltoheptaose | - |
Sulfolobus acidocaldarius | ? | - |
? |  |
| 5.4.99.15 | maltopentaose | - |
Sulfolobus acidocaldarius | ? | - |
? | |
| 5.4.99.15 | Maltotetraose | - |
Sulfolobus acidocaldarius | ? | - |
? | |
| 5.4.99.15 | maltotriose | - |
Sulfolobus acidocaldarius | ? | - |
? | |
| 5.4.99.15 | additional information | possible mechanism of action: after converting the alpha-1,4-glucosidic linkage to an alpha-1,1-glucosidic linkage at the reducing end of maltooligosaccharide Glc(n) is able to release glucose and maltooligosaccharide Glc(n-1) residues. Then the intramolecular transglycosylation and the hydrolytic reaction continues with maltooligosaccharide Glc(n-1) until the initial maltooligosaccharide is reduced to maltose | Sulfolobus acidocaldarius | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.15 | 75 | - |
- |
Sulfolobus acidocaldarius |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.15 | 80 | - |
72 h, citrate-phosphate buffer, 38% loss of activity | Sulfolobus acidocaldarius |
| 5.4.99.15 | 90 | - |
1 h, 30% loss of activity | Sulfolobus acidocaldarius |
| 5.4.99.15 | 100 | - |
1 h, 65% loss of activity | Sulfolobus acidocaldarius |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.15 | 5 | - |
- |
Sulfolobus acidocaldarius |
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Release 2023.1 (January 2023)
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