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Literature summary extracted from

  • Jeong, J.J.; Fushinobu, S.; Ito, S.; Jeon, B.S.; Shoun, H.; Wakagi, T.
    Characterization of the cupin-type phosphoglucose isomerase from the hyperthermophilic archaeon Thermococcus litoralis (2003), FEBS Lett., 535, 200-204.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.9 overexpression in Escherichia coli Thermococcus litoralis

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.9 E98V the ratio of turnover number and Km-value is 31373fold lower than that of the wild-type enzyme. The absorption maximum at 420 nm as found in wild-type enzyme is completely lost. Mutant enzyme does not contain iron or zinc or other metal Thermococcus litoralis
5.3.1.9 H137A the ratio of turnover number and Km-value is 66.7fold lower than that of the wild-type enzyme. The absorption maximum at 420 nm as found in wild-type enzyme is completely lost. Mutant enzyme does not contain iron or zinc or other metal Thermococcus litoralis
5.3.1.9 H89A the ratio of turnover number and Km-value is 2712fold lower than that of the wild-type enzyme. The absorption maximum at 420 nm as found in wild-type enzyme is completely lost. Mutant enzyme does not contain iron or zinc or other metal Thermococcus litoralis
5.3.1.9 H91A the ratio of turnover number and Km-value is 66.7fold lower than that of the wild-type enzyme. The absorption maximum at 420 nm as found in wild-type enzyme is completely lost. Mutant enzyme does not contain iron or zinc or other metal Thermococcus litoralis

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.9 Cd2+ 10 mM, 96% inhibition, D-fructose 6-phosphate as substrate Thermococcus litoralis
5.3.1.9 Co2+ 10 mM, 59% inhibition, D-fructose 6-phosphate as substrate Thermococcus litoralis
5.3.1.9 Cu2+ 10 mM, 96% inhibition, D-fructose 6-phosphate as substrate Thermococcus litoralis
5.3.1.9 D-fructose 1,6-bisphosphate
-
 Thermococcus litoralis
5.3.1.9 D-Fructose 1-phosphate
-
 Thermococcus litoralis
5.3.1.9 D-gluconate 6-phosphate
-
 Thermococcus litoralis
5.3.1.9 D-mannose 6-phosphate
-
 Thermococcus litoralis
5.3.1.9 EDTA 10 mM, 78% inhibition, D-fructose 6-phosphate as substrate Thermococcus litoralis
5.3.1.9 K+ 10 mM, 18% inhibition, D-fructose 6-phosphate as substrate Thermococcus litoralis
5.3.1.9 Mn2+ 10 mM, 18% inhibition, D-fructose 6-phosphate as substrate Thermococcus litoralis
5.3.1.9 Ni2+ 10 mM, 84% inhibition, D-fructose 6-phosphate as substrate Thermococcus litoralis
5.3.1.9 Zn2+ 10 mM, 89% inhibition, D-fructose 6-phosphate as substrate Thermococcus litoralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.9 0.59
-
 D-fructose 6-phosphate 50°C, pH 7.5, mutant enzyme E89V Thermococcus litoralis
5.3.1.9 1.4
-
 D-fructose 6-phosphate 50°C, pH 7.5, mutant enzyme H137A Thermococcus litoralis
5.3.1.9 1.7
-
 D-fructose 6-phosphate 50°C, pH 7.5, wild-type enzyme Thermococcus litoralis
5.3.1.9 2.1
-
 D-fructose 6-phosphate 50°C, pH 7.5, mutant enzyme H91A Thermococcus litoralis
5.3.1.9 3.5
-
 D-fructose 6-phosphate 50°C, pH 7.5, mutant enzyme H89A Thermococcus litoralis
5.3.1.9 11.7
-
 D-glucose 6-phosphate 50°C, pH 7.5, wild-type enzyme Thermococcus litoralis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.9 Fe2+ 10 mM, 37°C, 156% enhancement of the activity of the wild-type enzyme, mutant enzymes H89A, H91A, E98V and H137A do not contain iron or zinc Thermococcus litoralis
5.3.1.9 Iron wild-type enzyme contains 1.25 mol iron per mol of dimer, mutant enzymes H89A, H91A, E98V and H137A do not contain iron or zinc Thermococcus litoralis
5.3.1.9 Zinc wild-type enzyme contains 0.24 mol zinc per mol of dimer Thermococcus litoralis

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.9 Thermococcus litoralis P84140
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.9
-
 Thermococcus litoralis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.9 47.3
-
 formation of D-glucose 6-phosphate Thermococcus litoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.9 fructose 6-phosphate
-
 Thermococcus litoralis D-glucose 6-phosphate
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.9 0.0003
-
 D-fructose 6-phosphate 50°C, pH 7.5, mutant enzyme E98V Thermococcus litoralis
5.3.1.9 0.021
-
 D-fructose 6-phosphate 50°C, pH 7.5, mutant enzyme H89A Thermococcus litoralis
5.3.1.9 0.34
-
 D-fructose 6-phosphate 50°C, pH 7.5, mutant enzyme H137A Thermococcus litoralis
5.3.1.9 0.5
-
 D-fructose 6-phosphate 50°C, pH 7.5, mutant enzyme H91A Thermococcus litoralis
5.3.1.9 26
-
 D-fructose 6-phosphate 50°C, pH 7.5, wild-type enzyme Thermococcus litoralis
5.3.1.9 42
-
 D-glucose 6-phosphate 50°C, pH 7.5, wild-type enzyme Thermococcus litoralis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.1.9 0.38
-
 D-gluconate 6-phosphate 50°C, pH 7.5, D-fructose 6-phosphate as substrate Thermococcus litoralis
5.3.1.9 2.3
-
 D-mannose 6-phosphate 50°C, pH 7.5, D-fructose 6-phosphate as substrate Thermococcus litoralis
5.3.1.9 4
-
 D-Fructose 1-phosphate 50°C, pH 7.5, D-fructose 6-phosphate as substrate Thermococcus litoralis
5.3.1.9 12
-
 D-fructose 1,6-bisphosphate 50°C, pH 7.5, D-fructose 6-phosphate as substrate Thermococcus litoralis