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Literature summary extracted from
- Activation of ADAM 12 protease by copper (2001), FEBS Lett., 506, 65-68.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.24.B10 | expression of the soluble enzyme form ADAM 12-S in COS cells | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.24.B10 | C179S | site-directed mutagenesis, mutant behaves identical to the wild-type concerning metal ions, therfore the cysteine-switch and activation by copper are 2 different components of enzyme activation | Homo sapiens |
| 3.4.24.B10 | additional information | elimination of the cysteine-switch by a point mutation in the propeptide has no effect on copper activation, whereas mutation of an unpaired cysteine residue in the catalytic domain resulted in a mutant form of ADAM 12-S that is insensitiv to copper | Homo sapiens |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.B10 | Cu2+ | activates, dependent on, multistep activation mechanism involving both furin cleavage and copper binding | Homo sapiens |  |
| 3.4.24.B10 | Zn2+ | activates, dependent on, enzyme contains a catalytically active zinc-binding domain | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.B10 | protein + H2O | Homo sapiens | - |
peptides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.B10 | Homo sapiens | O43184 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.24.B10 | proteolytic modification | autocleavage occurs near the junction of the prodomain and the catalytic domain upon inactivation of cysteine residues C179 and in presence of Cu2+ which is further promoted in presence of ascorbate, multistep activation mechanism involving both furin cleavage and copper binding | Homo sapiens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.B10 | recombinant from COS cells | Homo sapiens |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.4.24.B10 | proteolysis of proteins | active site zinc ion is coordinated by Cys179 | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.B10 | protein + H2O | - |
Homo sapiens | peptides | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.24.B10 | a disintegrin and metalloprotease-12 | - |
Homo sapiens |
| 3.4.24.B10 | ADAM 12-S | - |
Homo sapiens |
| 3.4.24.B10 | M12.212 | Merops-ID | Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.B10 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.B10 | 7 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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