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Literature summary extracted from

  • Kvaratskhelia, M.; Wardleworth, B.N.; White, M.F.
    Multiple Holliday junction resolving enzyme activities in the Crenarchaeota and Euryarchaeota (2001), FEBS Lett., 491, 243-246.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.10 Pyrococcus furiosus
-
-
-
3.1.21.10 Pyrococcus furiosus E7FHX4
-
-
3.1.21.10 Saccharolobus solfataricus
-
 P2
-
3.1.21.10 Saccharolobus solfataricus Q97YX6
-
-
3.1.21.10 Saccharolobus solfataricus P2
-
 P2
-
3.1.21.10 Saccharolobus solfataricus P2 Q97YX6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.10 partial Pyrococcus furiosus
3.1.21.10 partial Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.10 DNA + H2O synthetic mobile four way junction Jbm5 as a substrate. The Hjc activity cleaves all four strands of the junction, three nucleotides 3' of the point of strand exchange. Pyrococcus has two Holliday junction resolving enzymes with different specificity: the cellular Hjc enzyme, and in addition the Hjr enzyme (probably of viral origin) Pyrococcus furiosus hydrolyzed DNA
-
 ?
3.1.21.10 DNA + H2O synthetic mobile four way junction Jbm5 as a substrate. The Hjc activity cleaves all four strands of the junction, three nucleotides 3' of the point of strand exchange. Sulfolobus solfataricus has two Holliday junction resolving enzymes with different specificity: the cellular Hjc enzyme, and in addition the Hjr enzyme (probably of viral origin) Saccharolobus solfataricus hydrolyzed DNA
-
 ?
3.1.21.10 DNA + H2O synthetic mobile four way junction Jbm5 as a substrate. The Hjr enzyme cleaves all four arms of the junction significantly. Cleavage occurs one nucleotide 3' of the point of strand exchange. The organisms has two Holliday junction resolving enzymes with different specificity: the cellular Hjc enzyme, and in addition the Hjr enzyme (probably of viral origin) Pyrococcus furiosus hydrolyzed DNA
-
 ?
3.1.21.10 DNA + H2O synthetic mobile four way junction Jbm5 as a substrate. The Hjc activity cleaves all four strands of the junction, three nucleotides 3' of the point of strand exchange. Sulfolobus solfataricus has two Holliday junction resolving enzymes with different specificity: the cellular Hjc enzyme, and in addition the Hjr enzyme (probably of viral origin) Saccharolobus solfataricus P2 hydrolyzed DNA
-
 ?
3.1.21.10 DNA + H2O a fixed junction with heterologous arms and a defined point of strand exchange. Holliday junction resolving enzyme Hjc cleaves all four strands of the junction, three nucleotides 3' of the point of the strand exchange. Holliday junction resolving enzyme Hjr cleaves all four arms of the junction. Significant cleavage occurs one nucleotide 3' of the point of strand exchange Pyrococcus furiosus ?
-
 ?
3.1.21.10 DNA + H2O a fixed junction with heterologous arms and a defined point of strand exchange. Holliday junction resolving enzyme Hjccleaves all four strands of the junction, three nucleotides 3' of the point of the strand exchange. Holliday junction resolving enzyme Hje cleaves only two strands, two nucleotides 3' of the junction centre. Hje is specific for strands that adopt a continous conformation in the stacked form of the junction Saccharolobus solfataricus ?
-
 ?
3.1.21.10 DNA + H2O a fixed junction with heterologous arms and a defined point of strand exchange. Holliday junction resolving enzyme Hjccleaves all four strands of the junction, three nucleotides 3' of the point of the strand exchange. Holliday junction resolving enzyme Hje cleaves only two strands, two nucleotides 3' of the junction centre. Hje is specific for strands that adopt a continous conformation in the stacked form of the junction Saccharolobus solfataricus P2 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.10 Hjc
-
 Pyrococcus furiosus
3.1.21.10 Hjc
-
 Saccharolobus solfataricus
3.1.21.10 Hjc protein
-
 Pyrococcus furiosus
3.1.21.10 Hjc protein
-
 Saccharolobus solfataricus
3.1.21.10 Hje
-
 Saccharolobus solfataricus
3.1.21.10 Hjr
-
 Pyrococcus furiosus
3.1.21.10 Hjr protein
-
 Pyrococcus furiosus
3.1.21.10 Holliday junction resolving enzyme
-
 Pyrococcus furiosus
3.1.21.10 Holliday junction resolving enzyme
-
 Saccharolobus solfataricus
3.1.21.10 Holliday junction resolving enzyme Hjc
-
 Pyrococcus furiosus
3.1.21.10 Holliday junction resolving enzyme Hjc
-
 Saccharolobus solfataricus
3.1.21.10 Holliday junction resolving enzyme Hje
-
 Saccharolobus solfataricus
3.1.21.10 Holliday junction resolving enzyme Hjr
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.21.10 60
-
 assay at Pyrococcus furiosus
3.1.21.10 60
-
 assay at Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.10 7.5
-
 assay at Pyrococcus furiosus
3.1.21.10 7.5
-
 assay at Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
3.1.21.10 physiological function the enzyme is involved in the pathway for homologous recombination of the cellular genome, which includes the RadA protein for strand exchange, and the Hjc protein for junction resolution Saccharolobus solfataricus
3.1.21.10 physiological function the enzyme is involved in the pathway for homologous recombination of the cellular genome, which includesthe RadA protein for strand exchange, and the Hjc protein for junction resolution Pyrococcus furiosus