EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.22.34 | DNA and amino acid sequence determination and analysis | Mus musculus |
3.4.22.34 | overexpression in murine cell line COP5 as a C-terminally IgG-fusion protein | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.22.34 | C52A | reduced autocatalytic activity, no activity against other substrates | Homo sapiens |
3.4.22.34 | H150A | reduced autocatalytic activity, no activity against other substrates | Homo sapiens |
3.4.22.34 | H47A | reduced autocatalytic activity, no activity against other substrates | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.34 | additional information | - |
additional information | - |
Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.22.34 | 48000 | - |
x * 48000, autocatalytically processed enzyme, SDS-PAGE | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.34 | protein + H2O | Homo sapiens | - |
peptides | - |
? | |
3.4.22.34 | protein + H2O | Mus musculus | - |
peptides | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.34 | Homo sapiens | - |
- |
- |
3.4.22.34 | Mus musculus | O89017 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.22.34 | proteolytic modification | autocatalytic activation by cleavage of aspartic acid residues, overview | Homo sapiens |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.34 | recombinant IgG-fusion protein from cell line COP5 | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.22.34 | additional information | - |
- |
Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.34 | benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O | - |
Homo sapiens | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
3.4.22.34 | legumain + H2O | autocatalysis | Homo sapiens | processed legumain + N-terminal and C-terminal prodomains | - |
? | |
3.4.22.34 | additional information | no activity with benzyloxycarbonyl-Gly-Ala-Gln-4-methylcoumarin-7-amide | Homo sapiens | ? | - |
? | |
3.4.22.34 | protein + H2O | - |
Homo sapiens | peptides | - |
? | |
3.4.22.34 | protein + H2O | - |
Mus musculus | peptides | - |
? | |
3.4.22.34 | succinyl-Tyr-Val-Ala-Asn-4-methylcoumarin-7-amide + H2O | - |
Homo sapiens | succinyl-Tyr-Val-Ala-Asn + 7-amino-4-methylcoumarin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.22.34 | ? | x * 48000, autocatalytically processed enzyme, SDS-PAGE | Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.34 | 25 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.34 | additional information | - |
additional information | - |
Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.34 | 6.1 | - |
recombinant enzyme, dependent on buffer system | Homo sapiens |