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Literature summary extracted from
- The proregion of papaya proteinase IV inhibits Colorado potato beetle digestive cysteine proteinases (1998), FEBS Lett., 434, 401-405.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.22.25 | biotechnology | plant protease proregions have a potential as regulators of cysteine proteinases in biotechnological systems and to target proteases of pests | Carica papaya |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.22.25 | expression of the proregion of the enzyme in Escherichia coli | Carica papaya |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.25 | additional information | Carica papaya | the recombinant proregion of the enzyme can inhibit the cysteine protease of larvae of the Colorado beetle, Leptinotarsa decemlineata, analoguesly to specific inhibitor oryzacystatin I, inhibitory effect is affected by temperature | ? | - |
? |  |
| 3.4.22.25 | protein + H2O | Carica papaya | - |
peptides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.25 | Carica papaya | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.25 | additional information | the recombinant proregion of the enzyme can inhibit the cysteine protease of larvae of the Colorado beetle, Leptinotarsa decemlineata, analoguesly to specific inhibitor oryzacystatin I, inhibitory effect is affected by temperature | Carica papaya | ? | - |
? | |
| 3.4.22.25 | protein + H2O | - |
Carica papaya | peptides | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.22.25 | Glycyl endopeptidase | - |
Carica papaya |
| 3.4.22.25 | Papaya proteinase IV | - |
Carica papaya |
| 3.4.22.25 | PPIV | - |
Carica papaya |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.25 | 37 | - |
recombinant proregion, 60 min, stable | Carica papaya |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.25 | 8 | - |
assay at | Carica papaya |
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Release 2023.1 (January 2023)
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