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Literature summary extracted from

  • Purcarea, C.; Herve, G.; Cunin, R.; Evans, D.R.
    Cloning, expression, and structure analysis of carbamate kinase-like carbamoyl phosphate synthetase from Pyrococcus abyssi (2001), Extremophiles, 5, 229-239.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.16 expression in Escherichia coli and Schizosaccharomyces pombe Pyrococcus abyssi

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.16 additional information construction of deletion mutant lacking the last 13 residues of the carboxyl end, 70% decrease of the enzyme activity in comparison of the full-length protein Pyrococcus abyssi

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.16 cytidine triphosphate
-
 Pyrococcus abyssi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.16 0.0372
-
 ATP pH 8.0, deletion mutant lacking the last 13 residues of the carboxyl end Pyrococcus abyssi
6.3.4.16 0.06
-
 ATP pH 8.0, recombinant enzyme Pyrococcus abyssi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.16 34360
-
-
 Pyrococcus abyssi

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.16 Pyrococcus abyssi Q9UWF8
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.16 0.014
-
 recombinant enzyme expressed in Schizosaccharomyces pombe Pyrococcus abyssi
6.3.4.16 0.12
-
 deletion mutant lacking the last 13 residues of the carboxyl end Pyrococcus abyssi
6.3.4.16 0.4
-
 recombinant enzyme expressed in Escherischia coli Pyrococcus abyssi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.16 ATP + carbamate
-
 Pyrococcus abyssi ADP + carbamoyl phosphate
-
 r
6.3.4.16 ATP + NH3 + CO2 + H2O three step reaction Pyrococcus abyssi ADP + phosphate + carbamoyl phosphate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
6.3.4.16 carbamate kinase-like carbamoyl phosphate synthetase this enzyme is a intermediary form between carbamate kinase and carbamoyl phosphate synthetase Pyrococcus abyssi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.16 additional information
-
 grows optimally at temperatures around 100°C Pyrococcus abyssi
6.3.4.16 90
-
-
 Pyrococcus abyssi