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Literature summary extracted from

  • Stepanov, V.G.; Nyborg, J.
    tmRNA from Thermus thermophilus. Interaction with alanyl-tRNA synthetase and elongation factor Tu (2003), Eur. J. Biochem., 270, 463-475.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.7 additional information elongation factor Tu protects acylated tRNAAla against hydrolysis of the alanyl ester bond Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.7 elongation factor Tu strongly inhibits the aminoacylation of tmRNA, deviation from the mechanism of aminoacyl ester bond protection upon formation of the canonical ternary complx between the elongation gactor Tu, nucleotide cofactor and aminoacyl-tRNA Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.7 additional information
-
 additional information kinetics Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.7 195000
-
 gel filtration Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.7 ATP + L-alanine + tRNAAla Thermus thermophilus
-
 AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
6.1.1.7 ATP + L-alanine + tRNAAla Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 AMP + diphosphate + L-alanyl-tRNAAla
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.7 Thermus thermophilus
-
-
-
6.1.1.7 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.7
-
 Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.7 0.105
-
 purified enzyme Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.7 ATP + L-alanine + tmRNA recombinant transfer messenger RNA substrate from Thermus thermophilus expressed in Escherichia coli Thermus thermophilus AMP + diphosphate + L-alanyl-tmRNA
-
 ?
6.1.1.7 ATP + L-alanine + tmRNA recombinant transfer messenger RNA substrate from Thermus thermophilus expressed in Escherichia coli Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-alanyl-tmRNA
-
 ?
6.1.1.7 ATP + L-alanine + tRNAAla
-
 Thermus thermophilus AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
6.1.1.7 ATP + L-alanine + tRNAAla
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-alanyl-tRNAAla
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.7 Ala-tRNA synthetase
-
 Thermus thermophilus
6.1.1.7 Alanine transfer RNA synthetase
-
 Thermus thermophilus
6.1.1.7 Alanine translase
-
 Thermus thermophilus
6.1.1.7 Alanine tRNA synthetase
-
 Thermus thermophilus
6.1.1.7 Alanine-transfer RNA ligase
-
 Thermus thermophilus
6.1.1.7 alanine-tRNA ligase
-
 Thermus thermophilus
6.1.1.7 Alanyl-transfer ribonucleate synthetase
-
 Thermus thermophilus
6.1.1.7 Alanyl-transfer ribonucleic acid synthetase
-
 Thermus thermophilus
6.1.1.7 Alanyl-transfer RNA synthetase
-
 Thermus thermophilus
6.1.1.7 Alanyl-tRNA synthetase
-
 Thermus thermophilus
6.1.1.7 AlaRS
-
 Thermus thermophilus
6.1.1.7 Synthase, alanyl-transfer ribonucleate
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.7 50
-
-
 Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.1.1.7 25 75 half-maximal activity at 25°C and 75°C with substrate tRNAAla, a sharp 345fold drop of activity occurs at 70°C, temperature profile Thermus thermophilus
6.1.1.7 35 65 half-maximal activity at 35°C and 65°C with substrate tmRNA, a sharp 345fold drop of activity occurs at 40°C, temperature profile Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.7 0.1
-
 tmRNA pH 7.6, 50°C Thermus thermophilus
6.1.1.7 2.2
-
 tRNAAla pH 7.6, 50°C Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.7 7.6
-
 assay at Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.7 ATP
-
 Thermus thermophilus