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Literature summary extracted from

  • Masson, P.; Nachon, F.; Bartels, C.F.; Froment, M.T.; Ribes, F.; Matthews, C.; Lockridge, O.
    High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine (2003), Eur. J. Biochem., 270, 315-324.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.8
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.8 A328F mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens
3.1.1.8 A328G mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens
3.1.1.8 A328I mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens
3.1.1.8 A328W mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens
3.1.1.8 A328Y mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens
3.1.1.8 E197Q mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens
3.1.1.8 L286W mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens
3.1.1.8 V288W mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens
3.1.1.8 Y332A mutant enzyme retains activity at pH 5 under conditions of excess substrate similar to the wild-type enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.8
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.8 an acylcholine + H2O = choline + a carboxylate mechanism Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.8 plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 butyrylthiocholine + H2O
-
 Homo sapiens thiocholine + butyrate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.8 0.016
-
 butyrylthiocholine pH 5, wild-type enzyme, absence of excess substrate Homo sapiens
3.1.1.8 0.05
-
 butyrylthiocholine pH 5, A328I mutant, presence of excess substrate Homo sapiens
3.1.1.8 0.4
-
 butyrylthiocholine pH 5, A328W and E197Q mutant, absence of excess substrate Homo sapiens
3.1.1.8 2
-
 butyrylthiocholine pH 5, L286W mutant, absence of excess substrate Homo sapiens
3.1.1.8 20.5
-
 butyrylthiocholine pH 5, A328I mutant, absence of excess substrate Homo sapiens
3.1.1.8 22.3
-
 butyrylthiocholine pH 5, A328G mutant, absence of excess substrate Homo sapiens
3.1.1.8 41.4
-
 butyrylthiocholine pH 5, Y332A mutant, presence of excess substrate Homo sapiens
3.1.1.8 46.7
-
 butyrylthiocholine pH 5, wild-type enzyme, absence of excess substrate Homo sapiens
3.1.1.8 48.3
-
 butyrylthiocholine pH 5, Y332A mutant, absence of excess substrate Homo sapiens
3.1.1.8 53.3
-
 butyrylthiocholine pH 5, A328W and E197Q mutant, absence of excess substrate Homo sapiens
3.1.1.8 71.7
-
 butyrylthiocholine pH 5, L286W mutant, absence of excess substrate Homo sapiens
3.1.1.8 105
-
 butyrylthiocholine pH 5, V288W mutant, absence of excess substrate Homo sapiens
3.1.1.8 110
-
 butyrylthiocholine pH 5, A328F mutant, absence of excess substrate Homo sapiens
3.1.1.8 290
-
 butyrylthiocholine pH 5, L286W mutant, presence of excess substrate Homo sapiens
3.1.1.8 920
-
 butyrylthiocholine pH 5, A328F mutant, presence of excess substrate Homo sapiens
3.1.1.8 1048
-
 butyrylthiocholine pH 5, V288W mutant, presence of excess substrate Homo sapiens
3.1.1.8 1050
-
 butyrylthiocholine pH 5, V288W mutant, presence of excess substrate Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.8 5
-
 enzyme retains activity at pH 5 and below under conditions of excess substrate activation Homo sapiens