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Literature summary extracted from

  • Sauvageot, N.; Pichereau, V.; Louarme, L.; Hartke, A.; Auffray, Y.; Laplace, J.M.
    Purification, characterization and subunits identification of the diol dehydratase of Lactobacillus collinoides (2002), Eur. J. Biochem., 269, 5731-5737.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.28 expression in Escherichia coli of the three genes encoding the subunits of the enzyme. Recombinant protein cannot form an active complex Secundilactobacillus collinoides

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.28 Ca2+ 10 mM, complete inhibition Secundilactobacillus collinoides
4.2.1.28 cyanocobalamin competitive inhibitor Secundilactobacillus collinoides
4.2.1.28 glycerol suicide inhibitor. The linear increase in beta-hydroxypropionaldehyde formation ceases after 4 min Secundilactobacillus collinoides
4.2.1.28 Mg2+ 10 mM, slight inhibitor Secundilactobacillus collinoides
4.2.1.28 additional information no inhibition with Li+ and NH4+ Secundilactobacillus collinoides
4.2.1.28 Na+ 10 mM, complete inhibition Secundilactobacillus collinoides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.28 0.008
-
 coenzyme B12
-
 Secundilactobacillus collinoides
4.2.1.28 1.6
-
 1,2-propanediol
-
 Secundilactobacillus collinoides
4.2.1.28 5.5
-
 1,2-ethanediol
-
 Secundilactobacillus collinoides
4.2.1.28 8.3
-
 glycerol
-
 Secundilactobacillus collinoides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.28 22000
-
 alpha2beta2gamma2, 2 * 63000 + 2 * 28000 + 2 * 22000, SDS-PAGE Secundilactobacillus collinoides
4.2.1.28 28000
-
 alpha2beta2gamma2, 2 * 63000 + 2 * 28000 + 2 * 22000, SDS-PAGE Secundilactobacillus collinoides
4.2.1.28 63000
-
 alpha2beta2gamma2, 2 * 63000 + 2 * 28000 + 2 * 22000, SDS-PAGE Secundilactobacillus collinoides
4.2.1.28 207000
-
 gel filtration Secundilactobacillus collinoides

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.28 Secundilactobacillus collinoides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.28 ammonium sulfate precipitation and gel filtration Secundilactobacillus collinoides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.28 0.22
-
 37ºC, pH 7.2, crude extract Secundilactobacillus collinoides
4.2.1.28 0.77
-
 37ºC, pH 7.2, purified enzyme Secundilactobacillus collinoides

Storage Stability

EC Number Storage Stability Organism
4.2.1.28 -20ºC, stable Secundilactobacillus collinoides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.28 1,2-ethanediol
-
 Secundilactobacillus collinoides acetaldehyde
-
 ?
4.2.1.28 1,2-propanediol
-
 Secundilactobacillus collinoides propanal
-
 ?
4.2.1.28 glycerol
-
 Secundilactobacillus collinoides beta-hydroxypropionaldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.28 hexamer alpha2beta2gamma2, 2 * 63000 + 2 * 28000 + 2 * 22000, SDS-PAGE Secundilactobacillus collinoides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.28 8.7
-
-
 Secundilactobacillus collinoides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.28 8.5 9.2
-
 Secundilactobacillus collinoides

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.28 coenzyme B12 adenosylcobalamin Secundilactobacillus collinoides

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.28 0.0264
-
 cyanocobalamin
-
 Secundilactobacillus collinoides