BRENDA - Enzyme Database

Characterization and regulation of yeast Ca2+-dependent phosphatidylethanolamine-phospholipase D activity

Tang, X.; Waksman, M.; Ely, Y.; Liscovitch, M.; Eur. J. Biochem. 269, 3821-3830 (2002)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
Ca2+
required, 1 mM
Saccharomyces cerevisiae
3.1.4.4
H2O2
exposure of cells to H2O2 leads to transient increase in activity followed by 90% decrease
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.4.4
additional information
sec14 mutant, enzyme activity is elevated by 40% at restrictive temperature
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
Ba2+
1 mM, 15-25% inhibition
Saccharomyces cerevisiae
3.1.4.4
Co2+
1 mM, 50-60% inhibition
Saccharomyces cerevisiae
3.1.4.4
H2O2
exposure of cells to H2O2 leads to transient increase in activity followed by 90% decrease
Saccharomyces cerevisiae
3.1.4.4
Inositol
at 0.075 mM in culture medium,reduction of enzyme activity by 30-40%
Saccharomyces cerevisiae
3.1.4.4
Mn2+
1 mM, 45-55% inhibition
Saccharomyces cerevisiae
3.1.4.4
Zn2+
1 mM, 45% inhibition
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.4
cytosol
-
Saccharomyces cerevisiae
5829
-
3.1.4.4
membrane
associated to
Saccharomyces cerevisiae
16020
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
additional information
does not catalyze transphosphatidylation reaction with primary short-chain alcohols
651126
Saccharomyces cerevisiae
?
-
651126
Saccharomyces cerevisiae
?
3.1.4.4
phosphatidylethanolamine + H2O
-
651126
Saccharomyces cerevisiae
ethanolamine + phosphatidate
-
651126
Saccharomyces cerevisiae
?
3.1.4.4
phosphatidylserine + H2O
-
651126
Saccharomyces cerevisiae
L-serine + phosphatidate
-
651126
Saccharomyces cerevisiae
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
Ca2+
required, 1 mM
Saccharomyces cerevisiae
3.1.4.4
H2O2
exposure of cells to H2O2 leads to transient increase in activity followed by 90% decrease
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.4.4
additional information
sec14 mutant, enzyme activity is elevated by 40% at restrictive temperature
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
Ba2+
1 mM, 15-25% inhibition
Saccharomyces cerevisiae
3.1.4.4
Co2+
1 mM, 50-60% inhibition
Saccharomyces cerevisiae
3.1.4.4
H2O2
exposure of cells to H2O2 leads to transient increase in activity followed by 90% decrease
Saccharomyces cerevisiae
3.1.4.4
Inositol
at 0.075 mM in culture medium,reduction of enzyme activity by 30-40%
Saccharomyces cerevisiae
3.1.4.4
Mn2+
1 mM, 45-55% inhibition
Saccharomyces cerevisiae
3.1.4.4
Zn2+
1 mM, 45% inhibition
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.4
cytosol
-
Saccharomyces cerevisiae
5829
-
3.1.4.4
membrane
associated to
Saccharomyces cerevisiae
16020
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
additional information
does not catalyze transphosphatidylation reaction with primary short-chain alcohols
651126
Saccharomyces cerevisiae
?
-
651126
Saccharomyces cerevisiae
?
3.1.4.4
phosphatidylethanolamine + H2O
-
651126
Saccharomyces cerevisiae
ethanolamine + phosphatidate
-
651126
Saccharomyces cerevisiae
?
3.1.4.4
phosphatidylserine + H2O
-
651126
Saccharomyces cerevisiae
L-serine + phosphatidate
-
651126
Saccharomyces cerevisiae
?