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Literature summary extracted from

  • Lonn, A.; Gardonyi, M.; van Zyl, W.; Hahn-Hagerdal, B.; Otero, R.C.
    Cold adaptation of xylose isomerase from Thermus thermophilus through random PCR mutagenesis. Gene cloning and protein characterization (2002), Eur. J. Biochem., 269, 157-163.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.5 expression in Escherichia coli HB101 Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.5 E372G broader pH range and nine times higher turnover for D-xylose at 60°C than wild-type Thermus thermophilus
5.3.1.5 E372G/F163L broader pH range and nine times higher turnover for D-xylose at 60°C than wild-type Thermus thermophilus
5.3.1.5 E372G/V379A broader pH range and nine times higher turnover for D-xylose at 60°C than wild-type Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.5 xylitol
-
 Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 3.44
-
 D-xylose 60°C, pH 7, wild-type Thermus thermophilus
5.3.1.5 25.1
-
 D-xylose 60°C, pH 7, mutant E372G/V379A Thermus thermophilus
5.3.1.5 28.7
-
 D-xylose 60°C, pH 7, mutant E372G Thermus thermophilus
5.3.1.5 52
-
 D-glucose 60°C, pH 7, mutant E372G/V379A Thermus thermophilus
5.3.1.5 89.4
-
 D-xylose 60°C, pH 7, mutant E372G/F163L Thermus thermophilus
5.3.1.5 130.8
-
 D-glucose 60°C, pH 7, mutant E372G/F163L Thermus thermophilus
5.3.1.5 146.8
-
 D-glucose 60°C, pH 7, wild-type Thermus thermophilus
5.3.1.5 171.8
-
 D-glucose 60°C, pH 7, mutant E372G Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.5 Co2+
-
 Thermus thermophilus
5.3.1.5 Mg2+
-
 Thermus thermophilus
5.3.1.5 Mn2+
-
 Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Glucose
-
 Thermus thermophilus D-Fructose
-
 r
5.3.1.5 D-Xylose
-
 Thermus thermophilus D-Xylulose
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.3.1.5 XI
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.5 75
-
 mutants E372G/V379A Thermus thermophilus
5.3.1.5 90
-
 wild-type Thermus thermophilus
5.3.1.5 95
-
 mutants E372G/F163L and E372G Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.5 0.7
-
 D-glucose 60°C, pH 7, mutant E372G/F163L Thermus thermophilus
5.3.1.5 0.833
-
 D-glucose 60°C, pH 7, wild-type Thermus thermophilus
5.3.1.5 16.3
-
 D-glucose 60°C, pH 7, wild-type Thermus thermophilus
5.3.1.5 23.8
-
 D-glucose 60°C, pH 7, mutant E372G Thermus thermophilus
5.3.1.5 39.9
-
 D-glucose 60°C, pH 7, mutant E372G/V379A Thermus thermophilus
5.3.1.5 46.6
-
 D-xylose 60°C, pH 7, wild-type Thermus thermophilus
5.3.1.5 66.9
-
 D-glucose 60°C, pH 7, mutant E372G/F163L Thermus thermophilus
5.3.1.5 70.8
-
 D-xylose 60°C, pH 7, mutant E372G/F163L Thermus thermophilus
5.3.1.5 88.7
-
 D-glucose 60°C, pH 7, mutant E372G Thermus thermophilus
5.3.1.5 257.5
-
 D-xylose 60°C, pH 7, mutant E372G/V379A Thermus thermophilus
5.3.1.5 258
-
 D-xylose 60°C, pH 7, mutant E372G/V379A Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.5 7
-
-
 Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.5 5 10 mutants E372G/V379A, E372G/F163L and E372G Thermus thermophilus
5.3.1.5 5 8 wild-type Thermus thermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.1.5 4.6
-
 xylitol 60°C, pH 7, wild-type Thermus thermophilus
5.3.1.5 33.2
-
 xylitol 60°C, pH 7, mutant E372G/V379A Thermus thermophilus
5.3.1.5 68.7
-
 xylitol 60°C, pH 7, mutant E372G Thermus thermophilus
5.3.1.5 1174
-
 xylitol 60°C, pH 7, mutant E372G/F163L Thermus thermophilus