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Literature summary extracted from

  • Lindermayr, C.; Mollers, B.; Fliegmann, J.; Uhlmann, A.; Lottspeich, F.; Meimberg, H.; Ebel, J.
    Divergent members of a soybean (Glycine max L.) 4-coumarate:coenzyme A ligase gene family (2002), Eur. J. Biochem., 269, 1304-1315.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 expressed in Escherichia coli as His-tag fusion protein Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.008
-
 ferulate
-
 Glycine max
6.2.1.12 0.009
-
 4-coumarate
-
 Glycine max
6.2.1.12 0.01
-
 4-coumarate
-
 Glycine max
6.2.1.12 0.011
-
 sinapate
-
 Glycine max
6.2.1.12 0.013
-
 caffeate
-
 Glycine max
6.2.1.12 0.022
-
 4-coumarate
-
 Glycine max
6.2.1.12 0.033
-
 caffeate
-
 Glycine max
6.2.1.12 0.034
-
 caffeate
-
 Glycine max
6.2.1.12 0.042
-
 4-coumarate
-
 Glycine max
6.2.1.12 0.05
-
 caffeate
-
 Glycine max
6.2.1.12 0.083
-
 3,4-dimethoxycinnamate
-
 Glycine max
6.2.1.12 0.14
-
 ferulate
-
 Glycine max
6.2.1.12 0.26
-
 cinnamate
-
 Glycine max
6.2.1.12 1.1
-
 cinnamate
-
 Glycine max
6.2.1.12 1.3
-
 ferulate
-
 Glycine max
6.2.1.12 1.7
-
 cinnamate
-
 Glycine max
6.2.1.12 3.1
-
 ferulate
-
 Glycine max
6.2.1.12 4.4
-
 cinnamate
-
 Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Glycine max plant phenylpropanoid metabolism AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Glycine max P31687 4CL4, cDNA; four isoforms 4CL1-4
-
6.2.1.12 Glycine max Q8S564 4CL1, cDNA; four isoforms 4CL1-4
-
6.2.1.12 Glycine max Q8S5C1 4CL2, cDNA; four isoforms 4CL1-4
-
6.2.1.12 Glycine max Q8S5C2 4CL3, genomic DNA; four isoforms 4CL1-4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12 recombinant protein using His-tag Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 3,4-dimethoxycinnamate + CoA
-
 Glycine max AMP + diphosphate + 3,4-dimethoxycinnamoyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Glycine max AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA plant phenylpropanoid metabolism Glycine max AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Glycine max AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA
-
 Glycine max AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA
-
 Glycine max AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + sinapate + CoA
-
 Glycine max AMP + diphosphate + sinapoyl-CoA
-
 ?
6.2.1.12 additional information no reaction with 3,4-dimethoxycinnamate and sinapate Glycine max ?
-
 ?