Literature summary extracted from

Kannemeier, C.; Feussner, A.; Stohr, H.A.; Weisse, J.; Preissner, K.T.; Romisch, J.
Factor VII and single-chain plasminogen activator-activating protease: activation and autoactivation of the proenzyme (2001), Eur. J. Biochem., 268, 3789-3796.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
3.4.21.B1	heparin	enhances autoactivation	Homo sapiens

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.4.21.B1	Ca2+	inhibits autoactivation and stabilizes the proenzyme	Homo sapiens

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.4.21.B1	58000	60000	nonreducing PAGE, proenzyme	Homo sapiens
3.4.21.B1	60000	-	gel-filtration, proenzyme	Homo sapiens
3.4.21.B1	64000	-	SDS-PAGE, proenzyme	Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.4.21.B1	Protein + H2O	Homo sapiens	-	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.21.B1	Homo sapiens	-	-	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
3.4.21.B1	glycoprotein	only N-terminal portion is glycosylated, 3.5% carbohydrate content represented by N-glucosamine, N-galactosamine, galactose, mannose, glucose and fucose	Homo sapiens
3.4.21.B1	proteolytic modification	autoactivation leads to the typical protease two-chain pattern and subsequent degradation products	Homo sapiens

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.21.B1	95% purity	Homo sapiens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.21.B1	additional information	enzyme displays pro-urokinase-activating properties as well as factor VII-activating protease activity	Homo sapiens	?	-	?
3.4.21.B1	Protein + H2O	-	Homo sapiens	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.4.21.B1	factor VII-activating protease	-	Homo sapiens
3.4.21.B1	FSAP	-	Homo sapiens

pI Value

EC Number	Organism	Comment	pI Value Maximum	pI Value
3.4.21.B1	Homo sapiens	isoelectric focusing, pH gradient 3-7	-	4.9

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Release 2023.1 (January 2023)