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Literature summary extracted from

  • Klemencic, I.; Carmona, A.K.; Cezari, M.H.; Juliano, M.A.; Juliano, L.; Guncar, G.; Turk, D.; Krizaj, I.; Turk, V.; Turk, B.
    Biochemical characterization of human cathepsin X revealed that the enzyme is an exopeptidase, acting as carboxymonopeptidase or carboxydipeptidase (2000), Eur. J. Biochem., 267, 5404-5412.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.18.1 CA-074
-
 Homo sapiens
3.4.18.1 chicken cystatin
-
 Homo sapiens
3.4.18.1 cystatin C
-
 Homo sapiens
3.4.18.1 GFG-semicarbazone
-
 Homo sapiens
3.4.18.1 Stefin A
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.18.1 0.001
-
 (2,4-dinitrophenyl)-GFFW pH 5.1, 37°C Homo sapiens
3.4.18.1 0.0018
-
 (2,4-dinitrophenyl)-GFFGW pH 5.1, 37°C Homo sapiens
3.4.18.1 0.0024
-
 (2,4-dinitrophenyl)-GFRFW pH 5.1, 37°C Homo sapiens
3.4.18.1 0.0036
-
 (2,4-dinitrophenyl)-GFFRW pH 5.1, 37°C Homo sapiens
3.4.18.1 0.0037
-
 ortho-aminobenzoyl-Phe-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro pH 5.1, 37°C Homo sapiens
3.4.18.1 0.0042
-
 (2,4-dinitrophenyl)-GFRW pH 5.1, 37°C Homo sapiens
3.4.18.1 0.0046
-
 (2,4-dinitrophenyl)-GRFFW pH 5.1, 37°C Homo sapiens
3.4.18.1 0.0083
-
 ortho-aminobenzoyl-Arg-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + pH 5.1, 37°C Homo sapiens
3.4.18.1 0.0105
-
 ortho-aminobenzoyl-Lys-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro pH 5.1, 37°C Homo sapiens
3.4.18.1 0.063
-
 CBZ-FR-7-amido-4-methylcoumarin pH 5.1, 37°C Homo sapiens
3.4.18.1 0.13
-
 CBZ-RR-7-amido-4-methylcoumarin pH 5.1, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.18.1 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.18.1 glycoprotein mature cathepsin X has at least one of the two potential glycosylation sites glycosylated Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.18.1 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.18.1 (2,4-dinitrophenyl)-GFFGW + H2O
-
 Homo sapiens (2,4-dinitrophenyl)-GFFG + L-Trp
-
 ?
3.4.18.1 (2,4-dinitrophenyl)-GFFRW + H2O
-
 Homo sapiens (2,4-dinitrophenyl)-GFFR + L-Trp
-
 ?
3.4.18.1 (2,4-dinitrophenyl)-GFFW + H2O
-
 Homo sapiens (2,4-dinitrophenyl)-GFF + L-Trp
-
 ?
3.4.18.1 (2,4-dinitrophenyl)-GFRFW + H2O
-
 Homo sapiens (2,4-dinitrophenyl)-GFR + L-Phe-L-Trp
-
 ?
3.4.18.1 (2,4-dinitrophenyl)-GFRW + H2O
-
 Homo sapiens (2,4-dinitrophenyl)-GFR + L-Trp
-
 ?
3.4.18.1 (2,4-dinitrophenyl)-GRFFW + H2O
-
 Homo sapiens (2,4-dinitrophenyl)-GRFF + L-Trp
-
 ?
3.4.18.1 (2-aminobenzoyl)-FFF + H2O
-
 Homo sapiens (2-aminobenzoyl)-FF + L-Phe
-
 ?
3.4.18.1 (2-aminobenzoyl)-FFFA + H2O
-
 Homo sapiens (2-aminobenzoyl)-FFF + L-Ala
-
 ?
3.4.18.1 (2-aminobenzoyl)-FFFP + H2O
-
 Homo sapiens (2-aminobenzoyl)-FFF + Pro
-
 ?
3.4.18.1 (2-aminobenzoyl)-FFFR + H2O
-
 Homo sapiens (2-aminobenzoyl)-FFF + L-Arg
-
 ?
3.4.18.1 (2-aminobenzoyl)-FFFR-NH2 + H2O
-
 Homo sapiens (2-aminobenzoyl)-FFF + Arg-amide
-
 ?
3.4.18.1 (2-aminobenzoyl)-FFFW + H2O
-
 Homo sapiens (2-aminobenzoyl)-FFF + L-Trp
-
 ?
3.4.18.1 (2-aminobenzoyl)-FFGW + H2O
-
 Homo sapiens (2-aminobenzoyl)-FFG + L-Trp
-
 ?
3.4.18.1 (2-aminobenzoyl)-FFRW + H2O
-
 Homo sapiens (2-aminobenzoyl)-FFR + L-Trp
-
 ?
3.4.18.1 (2-aminobenzoyl)-FFRW-NH2 + H2O
-
 Homo sapiens (2-aminobenzoyl)-FFR + L-Trp-amide
-
 ?
3.4.18.1 (2-aminobenzoyl)-FRFW-NH2 + H2O
-
 Homo sapiens (2-aminobenzoyl)-FR + Phe-Trp-amide
-
 ?
3.4.18.1 2-aminobenzoyl-Arg-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O
-
 Homo sapiens ?
-
 ?
3.4.18.1 2-aminobenzoyl-Leu-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O
-
 Homo sapiens ?
-
 ?
3.4.18.1 2-aminobenzoyl-Phe-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O
-
 Homo sapiens ?
-
 ?
3.4.18.1 3-aminobenzoyl-FR-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P + H2O
-
 Homo sapiens 3-aminobenzoyl-FR + (2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P
-
 ?
3.4.18.1 3-aminobenzoyl-LR-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P + H2O
-
 Homo sapiens 3-aminobenzoyl-LR + (2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P
-
 ?
3.4.18.1 3-aminobenzoyl-RR-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P + H2O
-
 Homo sapiens 3-aminobenzoyl-RR + (2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P
-
 ?
3.4.18.1 benzyloxycarbonyl-FR-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens benzyloxycarbonyl-FR + 7-amino-4-methylcoumarin
-
 ?
3.4.18.1 benzyloxycarbonyl-RR-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens benzyloxycarbonyl-RR + 7-amino-4-methylcoumarin
-
 ?
3.4.18.1 additional information the enzyme normally acts as a carboxymonopeptidase. The preference for Arg in the P1 position is so strong that cathepsin X cleaves substrates with Arg in antepenultimate position, acting also as a carboxypeptidase. A large hydrophobic residue such as Trp is preferred in the P1' position, although the enzyme cleaves all P1' residues investigated (Trp, Phe, Ala, Arg, Pro). The enzyme also cleaves the substrates with amide-blocked C-terminal carboxyl group with rates similar to that of the unblocked substrates Homo sapiens ?
-
 ?
3.4.18.1 ortho-aminobenzoyl-Arg-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O
-
 Homo sapiens ?
-
 ?
3.4.18.1 ortho-aminobenzoyl-Lys-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O
-
 Homo sapiens ?
-
 ?
3.4.18.1 ortho-aminobenzoyl-Phe-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.18.1 cathepsin Z
-
 Homo sapiens
3.4.18.1 cysteine-type carboxypeptidase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.18.1 0.007
-
 (2,4-dinitrophenyl)-GFFGW pH 5.1, 37°C Homo sapiens
3.4.18.1 0.02
-
 2-aminobenzoyl-Leu-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + pH 5.1, 37°C Homo sapiens
3.4.18.1 0.03
-
 (2,4-dinitrophenyl)-GFRFW pH 5.1, 37°C Homo sapiens
3.4.18.1 0.06
-
 2-aminobenzoyl-Arg-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + pH 5.1, 37°C Homo sapiens
3.4.18.1 0.07
-
 (2,4-dinitrophenyl)-GFFW pH 5.1, 37°C Homo sapiens
3.4.18.1 0.07
-
 2-aminobenzoyl-Phe-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + pH 5.1, 37°C Homo sapiens
3.4.18.1 0.22
-
 benzyloxycarbony-FR-4-methylcoumaryl-7-amide pH 5.1, 37°C Homo sapiens
3.4.18.1 0.23
-
 (2,4-dinitrophenyl)-GRFFW pH 5.1, 37°C Homo sapiens
3.4.18.1 0.3
-
 (2,4-dinitrophenyl)-GFFRW pH 5.1, 37°C Homo sapiens
3.4.18.1 0.63
-
 benzyloxycarbony-RR-4-methylcoumaryl-7-amide pH 5.1, 37°C Homo sapiens
3.4.18.1 0.7
-
 (2,4-dinitrophenyl)-GFRW pH 5.1, 37°C Homo sapiens
3.4.18.1 6.08
-
 benzyloxycarbony-RR-4-methylcoumaryl-7-amide pH 5.1, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.18.1 5
-
 reaction with 3-aminobenzoyl-FR-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P Homo sapiens
3.4.18.1 5 5.5 reaction with benzyloxycarbonyl-RR-4-methylcoumaryl-7-amide Homo sapiens
3.4.18.1 5 6 reaction with (2,4-dinitrophenyl)-GFFW Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.18.1 3.5 5.5 pH 3.5: about 50% of maximal activity, pH 5.5: about 50% of maximal activity, reaction with 3-aminobenzoyl-FR-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P Homo sapiens
3.4.18.1 4 6.5 pH 4.0: about 45% of maximal activity, reaction with benzyloxycarbonyl-RR-methylcoumarinamide Homo sapiens
3.4.18.1 4.5 6.5 pH 4.5: about 45% of maximal activity, pH 6.5: about 10% of maximal activity, reaction with (2,4-dinitrophenyl)-GFFW Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.18.1 0.0000017
-
 Stefin A pH 5.5, 25°C Homo sapiens
3.4.18.1 0.000012
-
 cystatin C pH 5.5, 25°C Homo sapiens
3.4.18.1 0.000015
-
 chicken cystatin pH 5.5, 25°C Homo sapiens
3.4.18.1 0.0166
-
 GFG-semicarbazone pH 5.5, 25°C Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.18.1 Homo sapiens isoelectric focusing
-
 5.1