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Literature summary extracted from

  • Matsumoto, H.; Rogi, T.; Yamashiro, K.; Kodama, S.; Tsuruoka, N.; Hattori, A.; Takio, K.; Mizutani, S.; Tsujimoto, M.
    Characterization of a recombinant soluble form of human placental leucine aminopeptidase/oxytocinase expressed in Chinese hamster ovary cells (2000), Eur. J. Biochem., 267, 46-52.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.3 leucine aminopeptidase/oxytocinase, expression in Chinese hamster ovary cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.3 1,10-phenanthroline 0.1 mM, 83% inhibition. 0.01 mM, 10% inhibition Homo sapiens
3.4.11.3 amastatin 0.1 mM, 96% inhibition. 0.01 mM, 79% inhibition Homo sapiens
3.4.11.3 bestatin 0.1 mM, 43% inhibition. 0.01 mM, 12% inhibition Homo sapiens
3.4.11.3 CuCl2 0.1 mM, 91% inhibition. 0.01 mM, 87% inhibition Homo sapiens
3.4.11.3 leupeptin 0.1 mM, 53% inhibition Homo sapiens
3.4.11.3 additional information EDTA has no effect Homo sapiens
3.4.11.3 pepstatin A 0.1 mM, 70% inhibition Homo sapiens
3.4.11.3 puromycin 0.1 mM, 36% inhibition. 0.01 mM, 5% inhibition Homo sapiens
3.4.11.3 ZnCl2 0.1 mM, 98% inhibition. 0.01 mM, 77% inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.3 0.0225
-
Leu-4-methylcoumaryl-7-amide pH 7.5, 37°C Homo sapiens
3.4.11.3 0.0436
-
S-benzyl-Cys-4-methylcoumaryl-7-amide pH 7.5, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.3 membrane bound to Homo sapiens 16020
-
3.4.11.3 soluble recombinant enzyme expressed in Chinese hamster ovary cells Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.3 Zinc enzyme contains 1 mol of zinc per mol of enzyme, contains the consensus HEXXH(X)18E motif of zinc-metallopeptidase proteins Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.3 170000
-
2 * 170000, SDS-PAGE Homo sapiens
3.4.11.3 280000
-
sedimentation velocity experiments Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.3 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.3 glycoprotein
-
Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.3
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.3 placenta
-
Homo sapiens
-
3.4.11.3 serum serum level of the enzyme increases with gestation Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.3 Arg-4-methylcoumaryl-7-amide + H2O
-
Homo sapiens Arg + 7-amino-4-methylcoumarin
-
?
3.4.11.3 Leu-4-methylcoumaryl-7-amide + H2O
-
Homo sapiens Leu + 7-amino-4-methylcoumarin
-
?
3.4.11.3 Lys-4-methylcoumaryl-7-amide + H2O
-
Homo sapiens Lys + 7-amino-4-methylcoumarin
-
?
3.4.11.3 Met-4-methylcoumaryl-7-amide + H2O
-
Homo sapiens Met + 7-amino-4-methylcoumarin
-
?
3.4.11.3 S-benzyl-Cys-4-methylcoumaryl-7-amide + H2O
-
Homo sapiens S-benzyl-Cys + 7-amino-4-methylcoumarin
-
?

Subunits

EC Number Subunits Comment Organism
3.4.11.3 dimer 2 * 170000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.11.3 leucine aminopeptidase/oxytocinase
-
Homo sapiens
3.4.11.3 P-LAP
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.3 0.04 1.97 Leu-4-methylcoumaryl-7-amide pH 7.5, 37°C Homo sapiens
3.4.11.3 9.18
-
Leu-4-methylcoumaryl-7-amide pH 7.5, 37°C Homo sapiens
3.4.11.3 12.4
-
S-benzyl-Cys-4-methylcoumaryl-7-amide pH 7.5, 37°C Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.3 0.0014
-
amastatin pH 7.5, 37°C Homo sapiens