Literature summary extracted from

Atencia, E.A.; Montes, M.; Gunther Sillero, M.A.; Sillero, A.
Several dinucleoside polyphosphates are acceptor substrates in the T4 RNA ligase catalyzed reaction (2000), Eur. J. Biochem., 267, 1707-1714.

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
6.5.1.3	0.4	-	5'-adenylated cytidine 5',3'-bisphosphate	pH 7.4, 30°C	Tequatrovirus T4
6.5.1.3	4	-	diguanosine tetraphosphate	pH 7.4, 30°C	Tequatrovirus T4

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
6.5.1.3	42000	-	SDS-PAGE	Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6.5.1.3	ATP + (ribonucleotide)n + (ribonucleotide)m	Tequatrovirus T4	-	AMP + diphosphate + (ribonucleotide)n+m	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.5.1.3	Tequatrovirus T4	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
6.5.1.3	-	Tequatrovirus T4

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6.5.1.3	5'-adenylated cytidine 5',3'-bisphosphate + diguanosine tetraphosphate	-	Tequatrovirus T4	Gp4GpCp + pCpGp4GpCp	-	?
6.5.1.3	ATP + (ribonucleotide)n + (ribonucleotide)m	-	Tequatrovirus T4	AMP + diphosphate + (ribonucleotide)n+m	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
6.5.1.3	T4 RNA ligase	-	Tequatrovirus T4

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Release 2023.1 (January 2023)