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Literature summary extracted from
- Specificity of the wound-induced leucine aminopeptidase (LAP-A) of tomato. Activity on dipeptide and tripeptide substrates (2000), Eur. J. Biochem., 267, 1178-1187.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.11.1 | overexpression of free and His-tagged enzyme in peptidase A-deficient Escherichia coli strain DS957 xerB:Tn5 | Solanum lycopersicum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.1 | 1.4 | - |
Arg-Leu | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum |  |
| 3.4.11.1 | 1.5 | - |
Leu-Gly | 37°C | Sus scrofa | |
| 3.4.11.1 | 1.9 | - |
Leu-Gly-Gly | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 2.2 | - |
Leu-Gly | free recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 2.9 | - |
Leu-Leu | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 3.2 | - |
Leu-Gly | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 3.3 | - |
Arg-Gly-Asp | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 4.5 | - |
Arg-Gly | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 8.6 | - |
Gly-Leu | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 9.6 | - |
Arg-Gly-Phe | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 11.2 | - |
Gly-Gly | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.1 | Mn2+ | activates | Escherichia coli | |
| 3.4.11.1 | Mn2+ | activates | Sus scrofa | |
| 3.4.11.1 | Mn2+ | activates | Solanum lycopersicum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 53500 | - |
x * 53500, SDS-PAGE | Escherichia coli |
| 3.4.11.1 | 54000 | - |
x * 54000, SDS-PAGE | Sus scrofa |
| 3.4.11.1 | 54868 | - |
x * 54868, free enzyme, sequence calculation, up to 6 subunits form the oligomer, oligomeric form is required for activity | Solanum lycopersicum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.1 | Escherichia coli | - |
- |
- |
| 3.4.11.1 | Solanum lycopersicum | - |
wound-induced enzyme | - |
| 3.4.11.1 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.1 | recombinant free and His-tagged enzymes from Escherichia coli, to near homogeneity | Solanum lycopersicum |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.4.11.1 | release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low | exoprotease, preference for N-terminal Leu, Met and Arg residues, low activity when Xaa is Gly or Trp, no activity when Xaa is Asp | Sus scrofa | |
| 3.4.11.1 | release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low | exoprotease, preference for N-terminal Leu, Met and Arg residues, low activity when Xaa is Gly or Tyr, no activity when Xaa is Asp | Solanum lycopersicum | |
| 3.4.11.1 | release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low | exoprotease, preference for N-terminal Leu, Met and Arg residues, low activity when Xaa is Gly, no activity when Xaa is Asp | Escherichia coli |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.11.1 | commercial preparation | - |
Sus scrofa | - |
| 3.4.11.1 | kidney | - |
Sus scrofa | - |
| 3.4.11.1 | leaf | enzyme accumulation in wounded leaves | Solanum lycopersicum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 4.3 | - |
purified enzyme, substrate Arg-Gly-Arg | Sus scrofa |
| 3.4.11.1 | 13 | - |
purified recombinant enzyme, substrate Arg-Gly-Arg | Solanum lycopersicum |
| 3.4.11.1 | 19 | - |
purified enzyme, substrate Arg-Gly-Ser | Sus scrofa |
| 3.4.11.1 | 21 | - |
purified enzyme, substrate Arg-Gly-Gly | Sus scrofa |
| 3.4.11.1 | 23.7 | - |
purified enzyme, substrate Arg-Gly-Leu or Arg-Gly-Phe | Sus scrofa |
| 3.4.11.1 | 39.9 | - |
purified recombinant enzyme, substrate Arg-Gly-Asp | Solanum lycopersicum |
| 3.4.11.1 | 47.7 | - |
purified recombinant enzyme, substrate Arg-Gly-Ser | Solanum lycopersicum |
| 3.4.11.1 | 53.2 | - |
purified recombinant enzyme, substrate Arg-Gly-Phe | Solanum lycopersicum |
| 3.4.11.1 | 53.5 | - |
purified enzyme, substrate Leu-Gly-Gly | Sus scrofa |
| 3.4.11.1 | 57.1 | - |
purified enzyme, substrate Arg-Gly | Sus scrofa |
| 3.4.11.1 | 68.7 | - |
purified enzyme, substrate Arg-Gly-Arg | Escherichia coli |
| 3.4.11.1 | 69.3 | - |
purified enzyme, substrate Arg-Gly-Asp | Sus scrofa |
| 3.4.11.1 | 70.9 | - |
purified recombinant enzyme, substrate Arg-Gly-Leu | Solanum lycopersicum |
| 3.4.11.1 | 77.5 | - |
purified recombinant enzyme, substrate Arg-Gly-Gly | Solanum lycopersicum |
| 3.4.11.1 | 130.4 | - |
purified recombinant enzyme, substrate Leu-Gly | Solanum lycopersicum |
| 3.4.11.1 | 139 | - |
purified recombinant enzyme, substrate Arg-Gly | Solanum lycopersicum |
| 3.4.11.1 | 170.3 | - |
purified enzyme, substrate Leu-Gly | Sus scrofa |
| 3.4.11.1 | 196.7 | - |
purified enzyme, substrate Arg-Gly-Ser | Escherichia coli |
| 3.4.11.1 | 241.6 | - |
purified recombinant enzyme, substrate Leu-Gly-Gly | Solanum lycopersicum |
| 3.4.11.1 | 300.1 | - |
purified enzyme, substrate Arg-Gly-Gly | Escherichia coli |
| 3.4.11.1 | 318 | - |
purified enzyme, substrate Leu-Gly | Escherichia coli |
| 3.4.11.1 | 355.1 | - |
purified enzyme, substrate Arg-Gly-Phe | Escherichia coli |
| 3.4.11.1 | 396.3 | - |
purified enzyme, substrate Arg-Gly-Leu | Escherichia coli |
| 3.4.11.1 | 443.1 | - |
purified enzyme, substrate Arg-Gly | Escherichia coli |
| 3.4.11.1 | 604.7 | - |
purified enzyme, substrate Leu-Gly-Gly | Escherichia coli |
| 3.4.11.1 | 790.5 | - |
purified enzyme, substrate Arg-Gly-Asp | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.1 | Ala-Gly + H2O | - |
Solanum lycopersicum | Ala + Gly | - |
? | |
| 3.4.11.1 | Ala-Gly + H2O | low activity | Escherichia coli | Ala + Gly | - |
? | |
| 3.4.11.1 | Ala-Gly + H2O | low activity | Sus scrofa | Ala + Gly | - |
? | |
| 3.4.11.1 | Ala-Leu + H2O | high activity | Escherichia coli | Ala + Leu | - |
? | |
| 3.4.11.1 | Ala-Leu + H2O | high activity | Sus scrofa | Ala + Leu | - |
? | |
| 3.4.11.1 | Ala-Leu + H2O | high activity | Solanum lycopersicum | Ala + Leu | - |
? | |
| 3.4.11.1 | Arg-Ala + H2O | - |
Escherichia coli | Arg + Ala | - |
? | |
| 3.4.11.1 | Arg-Ala + H2O | - |
Sus scrofa | Arg + Ala | - |
? | |
| 3.4.11.1 | Arg-Ala + H2O | - |
Solanum lycopersicum | Arg + Ala | - |
? | |
| 3.4.11.1 | Arg-Glu + H2O | low activity | Escherichia coli | Arg + Glu | - |
? | |
| 3.4.11.1 | Arg-Glu + H2O | low activity | Sus scrofa | Arg + Glu | - |
? | |
| 3.4.11.1 | Arg-Glu + H2O | low activity | Solanum lycopersicum | Arg + Glu | - |
? | |
| 3.4.11.1 | Arg-Gly + H2O | - |
Escherichia coli | Arg + Gly | - |
? | |
| 3.4.11.1 | Arg-Gly + H2O | - |
Sus scrofa | Arg + Gly | - |
? | |
| 3.4.11.1 | Arg-Gly + H2O | - |
Solanum lycopersicum | Arg + Gly | - |
? | |
| 3.4.11.1 | Arg-Gly + H2O | low activity | Escherichia coli | Arg + Gly | - |
? | |
| 3.4.11.1 | Arg-Gly + H2O | low activity | Sus scrofa | Arg + Gly | - |
? | |
| 3.4.11.1 | Arg-Gly + H2O | low activity | Solanum lycopersicum | Arg + Gly | - |
? | |
| 3.4.11.1 | Arg-Gly-Arg + H2O | low activity | Escherichia coli | Arg + Gly-Arg | - |
? | |
| 3.4.11.1 | Arg-Gly-Arg + H2O | low activity | Sus scrofa | Arg + Gly-Arg | - |
? | |
| 3.4.11.1 | Arg-Gly-Arg + H2O | low activity | Solanum lycopersicum | Arg + Gly-Arg | - |
? | |
| 3.4.11.1 | Arg-Gly-Asp + H2O | - |
Escherichia coli | Arg + Gly-Asp | - |
? | |
| 3.4.11.1 | Arg-Gly-Asp + H2O | - |
Sus scrofa | Arg + Gly-Asp | - |
? | |
| 3.4.11.1 | Arg-Gly-Asp + H2O | - |
Solanum lycopersicum | Arg + Gly-Asp | - |
? | |
| 3.4.11.1 | Arg-Gly-Gly + H2O | - |
Escherichia coli | Arg + Gly-Gly | - |
? | |
| 3.4.11.1 | Arg-Gly-Gly + H2O | - |
Sus scrofa | Arg + Gly-Gly | - |
? | |
| 3.4.11.1 | Arg-Gly-Gly + H2O | - |
Solanum lycopersicum | Arg + Gly-Gly | - |
? | |
| 3.4.11.1 | Arg-Gly-Leu + H2O | - |
Escherichia coli | Arg + Gly-Leu | - |
? | |
| 3.4.11.1 | Arg-Gly-Leu + H2O | - |
Sus scrofa | Arg + Gly-Leu | - |
? | |
| 3.4.11.1 | Arg-Gly-Leu + H2O | - |
Solanum lycopersicum | Arg + Gly-Leu | - |
? | |
| 3.4.11.1 | Arg-Gly-Phe + H2O | - |
Escherichia coli | Arg + Gly-Phe | - |
? | |
| 3.4.11.1 | Arg-Gly-Phe + H2O | - |
Sus scrofa | Arg + Gly-Phe | - |
? | |
| 3.4.11.1 | Arg-Gly-Phe + H2O | - |
Solanum lycopersicum | Arg + Gly-Phe | - |
? | |
| 3.4.11.1 | Arg-Gly-Ser + H2O | - |
Escherichia coli | Arg + Gly-Ser | - |
? | |
| 3.4.11.1 | Arg-Gly-Ser + H2O | - |
Sus scrofa | Arg + Gly-Ser | - |
? | |
| 3.4.11.1 | Arg-Gly-Ser + H2O | - |
Solanum lycopersicum | Arg + Gly-Ser | - |
? | |
| 3.4.11.1 | Arg-Ile + H2O | - |
Escherichia coli | Arg + Ile | - |
? | |
| 3.4.11.1 | Arg-Ile + H2O | - |
Sus scrofa | Arg + Ile | - |
? | |
| 3.4.11.1 | Arg-Ile + H2O | - |
Solanum lycopersicum | Arg + Ile | - |
? | |
| 3.4.11.1 | Arg-Leu + H2O | high activity | Escherichia coli | Arg + Leu | - |
? | |
| 3.4.11.1 | Arg-Leu + H2O | high activity | Sus scrofa | Arg + Leu | - |
? | |
| 3.4.11.1 | Arg-Leu + H2O | high activity | Solanum lycopersicum | Arg + Leu | - |
? | |
| 3.4.11.1 | Arg-Phe + H2O | - |
Escherichia coli | Arg + Phe | - |
? | |
| 3.4.11.1 | Arg-Phe + H2O | - |
Sus scrofa | Arg + Phe | - |
? | |
| 3.4.11.1 | Arg-Phe + H2O | - |
Solanum lycopersicum | Arg + Phe | - |
? | |
| 3.4.11.1 | Arg-Val + H2O | - |
Escherichia coli | Arg + Val | - |
? | |
| 3.4.11.1 | Arg-Val + H2O | - |
Sus scrofa | Arg + Val | - |
? | |
| 3.4.11.1 | Arg-Val + H2O | - |
Solanum lycopersicum | Arg + Val | - |
? | |
| 3.4.11.1 | Cys-Gly + H2O | low activity | Escherichia coli | Cys + Gly | - |
? | |
| 3.4.11.1 | Cys-Gly + H2O | low activity | Sus scrofa | Cys + Gly | - |
? | |
| 3.4.11.1 | Gly-Leu + H2O | low activity | Escherichia coli | Gly + Leu | - |
? | |
| 3.4.11.1 | Gly-Leu + H2O | low activity | Sus scrofa | Gly + Leu | - |
? | |
| 3.4.11.1 | Gly-Leu + H2O | low activity | Solanum lycopersicum | Gly + Leu | - |
? | |
| 3.4.11.1 | Gly-Phe + H2O | - |
Sus scrofa | Gly + Phe | - |
? | |
| 3.4.11.1 | Gly-Phe + H2O | - |
Solanum lycopersicum | Gly + Phe | - |
? | |
| 3.4.11.1 | Gly-Phe + H2O | low activity | Escherichia coli | Gly + Phe | - |
? | |
| 3.4.11.1 | Gly-Trp + H2O | low activity | Escherichia coli | Gly + Trp | - |
? | |
| 3.4.11.1 | Gly-Trp + H2O | low activity | Sus scrofa | Gly + Trp | - |
? | |
| 3.4.11.1 | Gly-Tyr + H2O | low activity | Escherichia coli | Gly + Tyr | - |
? | |
| 3.4.11.1 | Gly-Tyr + H2O | low activity | Sus scrofa | Gly + Tyr | - |
? | |
| 3.4.11.1 | Gly-Tyr + H2O | low activity | Solanum lycopersicum | Gly + Tyr | - |
? | |
| 3.4.11.1 | Gly-Val + H2O | low activity | Solanum lycopersicum | Gly + Val | - |
? | |
| 3.4.11.1 | His-Gly + H2O | - |
Escherichia coli | His + Gly | - |
? | |
| 3.4.11.1 | His-Gly + H2O | - |
Sus scrofa | His + Gly | - |
? | |
| 3.4.11.1 | His-Gly + H2O | - |
Solanum lycopersicum | His + Gly | - |
? | |
| 3.4.11.1 | His-Leu + H2O | - |
Sus scrofa | His + Leu | - |
? | |
| 3.4.11.1 | His-Leu + H2O | - |
Solanum lycopersicum | His + Leu | - |
? | |
| 3.4.11.1 | His-Leu + H2O | low activity | Escherichia coli | His + Leu | - |
? | |
| 3.4.11.1 | Leu-4-nitroanilide + H2O | - |
Escherichia coli | Leu + 4-nitroaniline | - |
? | |
| 3.4.11.1 | Leu-4-nitroanilide + H2O | - |
Sus scrofa | Leu + 4-nitroaniline | - |
? | |
| 3.4.11.1 | Leu-4-nitroanilide + H2O | - |
Solanum lycopersicum | Leu + 4-nitroaniline | - |
? | |
| 3.4.11.1 | Leu-Arg + H2O | - |
Escherichia coli | Leu + Arg | - |
? | |
| 3.4.11.1 | Leu-Arg + H2O | - |
Sus scrofa | Leu + Arg | - |
? | |
| 3.4.11.1 | Leu-Arg + H2O | - |
Solanum lycopersicum | Leu + Arg | - |
? | |
| 3.4.11.1 | Leu-Asn + H2O | - |
Escherichia coli | Leu + Asn | - |
? | |
| 3.4.11.1 | Leu-Asn + H2O | - |
Sus scrofa | Leu + Asn | - |
? | |
| 3.4.11.1 | Leu-Asn + H2O | - |
Solanum lycopersicum | Leu + Asn | - |
? | |
| 3.4.11.1 | Leu-Asp + H2O | low activity | Escherichia coli | Leu + Asp | - |
? | |
| 3.4.11.1 | Leu-Asp + H2O | low activity | Sus scrofa | Leu + Asp | - |
? | |
| 3.4.11.1 | Leu-Gly + H2O | - |
Escherichia coli | Leu + Gly | - |
? | |
| 3.4.11.1 | Leu-Gly + H2O | - |
Sus scrofa | Leu + Gly | - |
? | |
| 3.4.11.1 | Leu-Gly + H2O | - |
Solanum lycopersicum | Leu + Gly | - |
? | |
| 3.4.11.1 | Leu-Gly-Gly + H2O | - |
Escherichia coli | Leu + Gly-Gly | - |
? | |
| 3.4.11.1 | Leu-Gly-Gly + H2O | - |
Sus scrofa | Leu + Gly-Gly | - |
? | |
| 3.4.11.1 | Leu-Gly-Gly + H2O | - |
Solanum lycopersicum | Leu + Gly-Gly | - |
? | |
| 3.4.11.1 | Leu-Leu + H2O | - |
Escherichia coli | Leu + Leu | - |
? | |
| 3.4.11.1 | Leu-Leu + H2O | high activity | Sus scrofa | Leu + Leu | - |
? | |
| 3.4.11.1 | Leu-Leu + H2O | best dipeptide substrate | Solanum lycopersicum | Leu + Leu | - |
? | |
| 3.4.11.1 | Leu-Met + H2O | - |
Escherichia coli | Leu + Met | - |
? | |
| 3.4.11.1 | Leu-Met + H2O | - |
Sus scrofa | Leu + Met | - |
? | |
| 3.4.11.1 | Leu-Met + H2O | - |
Solanum lycopersicum | Leu + Met | - |
? | |
| 3.4.11.1 | Leu-Phe + H2O | - |
Escherichia coli | Leu + Phe | - |
? | |
| 3.4.11.1 | Leu-Phe + H2O | - |
Sus scrofa | Leu + Phe | - |
? | |
| 3.4.11.1 | Leu-Phe + H2O | - |
Solanum lycopersicum | Leu + Phe | - |
? | |
| 3.4.11.1 | Leu-Ser + H2O | - |
Escherichia coli | Leu + Ser | - |
? | |
| 3.4.11.1 | Leu-Ser + H2O | - |
Sus scrofa | Leu + Ser | - |
? | |
| 3.4.11.1 | Leu-Ser + H2O | - |
Solanum lycopersicum | Leu + Ser | - |
? | |
| 3.4.11.1 | Leu-Trp + H2O | - |
Escherichia coli | Leu + Trp | - |
? | |
| 3.4.11.1 | Leu-Trp + H2O | - |
Sus scrofa | Leu + Trp | - |
? | |
| 3.4.11.1 | Leu-Trp + H2O | - |
Solanum lycopersicum | Leu + Trp | - |
? | |
| 3.4.11.1 | Leu-Tyr + H2O | - |
Escherichia coli | Leu + Tyr | - |
? | |
| 3.4.11.1 | Leu-Tyr + H2O | - |
Sus scrofa | Leu + Tyr | - |
? | |
| 3.4.11.1 | Leu-Tyr + H2O | - |
Solanum lycopersicum | Leu + Tyr | - |
? | |
| 3.4.11.1 | Leu-Val + H2O | - |
Escherichia coli | Leu + Val | - |
? | |
| 3.4.11.1 | Leu-Val + H2O | - |
Sus scrofa | Leu + Val | - |
? | |
| 3.4.11.1 | Leu-Val + H2O | - |
Solanum lycopersicum | Leu + Val | - |
? | |
| 3.4.11.1 | Met-Leu + H2O | - |
Escherichia coli | Met + Leu | - |
? | |
| 3.4.11.1 | Met-Leu + H2O | high activity | Sus scrofa | Met + Leu | - |
? | |
| 3.4.11.1 | Met-Leu + H2O | high activity | Solanum lycopersicum | Met + Leu | - |
? | |
| 3.4.11.1 | additional information | substrate specificity study, Asp-Leu, Pro-Gly, Gly-Gly, Cys-Gly, Ser-Gly, Gly-Trp, Gly-Pro, Gly-His, Gly-Lys and Lys-Gly are poor substrates, Asp-Gly, Leu-Asp, Arg-Lys, Arg-Asp, Gly-Asn, Gly-Gln, and Leu-Pro are no substrates | Solanum lycopersicum | ? | - |
? | |
| 3.4.11.1 | additional information | substrate specificity study, Asp-Leu, Pro-Gly, Gly-Gly, Ser-Gly, Arg-Lys, Gly-Ala, Gly-Gln, Gly-His, Gly-Val and Lys-Gly are poor substrates, Asp-Gly, Arg-Asp, Gly-Arg, Gly-Asn, Gly-Lys, and Leu-Pro are no substrates | Sus scrofa | ? | - |
? | |
| 3.4.11.1 | additional information | substrate specificity study, Asp-Leu, Pro-Gly, Lys-Gly, Ser-Gly, Arg-Lys, Arg-Asp, Gly-Ala, Gly-Val, Gly-Trp, Gly-Gln, Gly-Gly, Gly-Lys, Gly-His, and Leu-Pro are poor substrates, Gly-Asn, Asp-Gly, Gly-Arg, and Gly-Asn are no substrates | Escherichia coli | ? | - |
? | |
| 3.4.11.1 | Phe-Gly + H2O | - |
Escherichia coli | Phe + Gly | - |
? | |
| 3.4.11.1 | Phe-Gly + H2O | - |
Sus scrofa | Phe + Gly | - |
? | |
| 3.4.11.1 | Phe-Gly + H2O | - |
Solanum lycopersicum | Phe + Gly | - |
? | |
| 3.4.11.1 | Phe-Leu + H2O | - |
Sus scrofa | Phe + Leu | - |
? | |
| 3.4.11.1 | Phe-Leu + H2O | - |
Solanum lycopersicum | Phe + Leu | - |
? | |
| 3.4.11.1 | Phe-Leu + H2O | high activity | Escherichia coli | Phe + Leu | - |
? | |
| 3.4.11.1 | Pro-Leu + H2O | - |
Escherichia coli | Pro + Leu | - |
? | |
| 3.4.11.1 | Pro-Leu + H2O | - |
Sus scrofa | Pro + Leu | - |
? | |
| 3.4.11.1 | Pro-Leu + H2O | - |
Solanum lycopersicum | Pro + Leu | - |
? | |
| 3.4.11.1 | Thr-Leu + H2O | - |
Escherichia coli | Thr + Leu | - |
? | |
| 3.4.11.1 | Thr-Leu + H2O | - |
Sus scrofa | Thr + Leu | - |
? | |
| 3.4.11.1 | Thr-Leu + H2O | - |
Solanum lycopersicum | Thr + Leu | - |
? | |
| 3.4.11.1 | Trp-Leu + H2O | - |
Solanum lycopersicum | Trp + Leu | - |
? | |
| 3.4.11.1 | Trp-Leu + H2O | low activity | Escherichia coli | Trp + Leu | - |
? | |
| 3.4.11.1 | Trp-Leu + H2O | low activity | Sus scrofa | Trp + Leu | - |
? | |
| 3.4.11.1 | Tyr-Gly + H2O | - |
Escherichia coli | Tyr + Gly | - |
? | |
| 3.4.11.1 | Tyr-Gly + H2O | - |
Sus scrofa | Tyr + Gly | - |
? | |
| 3.4.11.1 | Tyr-Gly + H2O | low activity | Solanum lycopersicum | Tyr + Gly | - |
? | |
| 3.4.11.1 | Tyr-Leu + H2O | - |
Escherichia coli | Tyr + Leu | - |
? | |
| 3.4.11.1 | Tyr-Leu + H2O | - |
Sus scrofa | Tyr + Leu | - |
? | |
| 3.4.11.1 | Tyr-Leu + H2O | low activity | Solanum lycopersicum | Tyr + Leu | - |
? | |
| 3.4.11.1 | Val-Gly + H2O | - |
Escherichia coli | Val + Gly | - |
? | |
| 3.4.11.1 | Val-Gly + H2O | - |
Sus scrofa | Val + Gly | - |
? | |
| 3.4.11.1 | Val-Gly + H2O | - |
Solanum lycopersicum | Val + Gly | - |
? | |
| 3.4.11.1 | Val-Leu + H2O | high activity | Escherichia coli | Val + Leu | - |
? | |
| 3.4.11.1 | Val-Leu + H2O | high activity | Sus scrofa | Val + Leu | - |
? | |
| 3.4.11.1 | Val-Leu + H2O | high activity | Solanum lycopersicum | Val + Leu | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.11.1 | ? | x * 54000, SDS-PAGE | Sus scrofa |
| 3.4.11.1 | ? | x * 53500, SDS-PAGE | Escherichia coli |
| 3.4.11.1 | oligomer | x * 54868, free enzyme, sequence calculation, up to 6 subunits form the oligomer, oligomeric form is required for activity | Solanum lycopersicum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.11.1 | LAP | - |
Escherichia coli |
| 3.4.11.1 | LAP | - |
Sus scrofa |
| 3.4.11.1 | LAP-A | - |
Solanum lycopersicum |
| 3.4.11.1 | leucine aminopeptidase | - |
Escherichia coli |
| 3.4.11.1 | leucine aminopeptidase | - |
Sus scrofa |
| 3.4.11.1 | leucine aminopeptidase | - |
Solanum lycopersicum |
| 3.4.11.1 | PepA peptidase A | - |
Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 37 | - |
assay at | Solanum lycopersicum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.1 | 3780 | - |
Arg-Gly-Asp | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 3781 | - |
Arg-Gly-Asp | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 5140 | - |
Gly-Leu | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 5141 | - |
Gly-Leu | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 6070 | - |
Arg-Gly-Phe | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 6073 | - |
Arg-Gly-Phe | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 10860 | - |
Gly-Gly | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 10900 | - |
Gly-Gly | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 13500 | - |
Arg-Gly | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 13520 | - |
Arg-Gly | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 13960 | - |
Leu-Gly-Gly | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 14000 | - |
Leu-Gly-Gly | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 14100 | - |
Leu-Gly | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 14400 | - |
Leu-Gly | free recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 14440 | - |
Leu-Gly | free recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 19380 | - |
Arg-Leu | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 19400 | - |
Arg-Leu | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 34490 | - |
Leu-Leu | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
| 3.4.11.1 | 34500 | - |
Leu-Leu | His-tagged recombinant enzyme, 37°C | Solanum lycopersicum | |
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Release 2023.1 (January 2023)
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