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Literature summary extracted from

  • Papir, G.; Spungin-Bialik, A.; Ben-Meir, D.; Fudim, E.; Gilboa, R.; Greenblatt, H.M.; Shoham, G.; Lessel, U.; Schomburg, D.; Ashkenazi, R.; Blumberg, S.
    Inhibition of Streptomyces griseus aminopeptidase and effects of calcium ions on catalysis and binding--comparisons with the homologous enzyme Aeromonas proteolytica aminopeptidase (1998), Eur. J. Biochem., 258, 313-319.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.11.24 Ca2+ stabilizes Streptomyces griseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.24 amastatin
-
 Streptomyces griseus
3.4.11.24 bestatin
-
 Streptomyces griseus
3.4.11.24 D-phenylalanine
-
 Streptomyces griseus
3.4.11.24 L-alanine
-
 Streptomyces griseus
3.4.11.24 L-arginine
-
 Streptomyces griseus
3.4.11.24 L-aspartate
-
 Streptomyces griseus
3.4.11.24 L-leucine
-
 Streptomyces griseus
3.4.11.24 L-leucine chloromethyl ketone
-
 Streptomyces griseus
3.4.11.24 L-methionine
-
 Streptomyces griseus
3.4.11.24 L-phenylalanine
-
 Streptomyces griseus
3.4.11.24 L-phenylalanine chloromethyl ketone
-
 Streptomyces griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.24 additional information
-
 additional information kinetics Streptomyces griseus
3.4.11.24 0.55
-
 L-leucine-4-nitroanilide pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
3.4.11.24 0.79
-
 L-valine-4-nitroanilide pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
3.4.11.24 2.15
-
 L-valine-4-nitroanilide pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
3.4.11.24 2.97
-
 L-leucine-4-nitroanilide pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
3.4.11.24 3.84
-
 L-alanine-4-nitroanilide pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
3.4.11.24 4.81
-
 L-alanine-4-nitroanilide pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.24 Ca2+ activates, enhances stability of the enzyme, modulates the enzyme activity and affinity towards substrates and inhibitors in a structure-dependent manner, binding structure Streptomyces griseus
3.4.11.24 Zn2+ 2 mol zinc per mol of enzyme, binding structure Streptomyces griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.24 29000 29730 native PAGE and mass spectrometry Streptomyces griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.24 peptide + H2O Streptomyces griseus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.24 Streptomyces griseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.24 from commercial product Streptomyces griseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.24 commercial preparation protease type XIV i.e. pronase E, a protease mixture form Streptomyces griseus Streptomyces griseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.24 additional information
-
-
 Streptomyces griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.24 L-alanine-4-nitroanilide + H2O
-
 Streptomyces griseus L-alanine + 4-nitroaniline
-
 ?
3.4.11.24 L-leucine-4-nitroanilide + H2O best of the amino acid 4-nitroanilide substrates Streptomyces griseus L-leucine + 4-nitroaniline
-
 ?
3.4.11.24 L-valine-4-nitroanilide + H2O
-
 Streptomyces griseus L-valine + 4-nitroaniline
-
 ?
3.4.11.24 peptide + H2O
-
 Streptomyces griseus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.24 monomer 1 * 29728-29731, mass spectrometry Streptomyces griseus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.24 M28.003 Merops-ID Streptomyces griseus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.24 70
-
 stable at Streptomyces griseus
3.4.11.24 75
-
 rate constant for inactivation Kin is 0.00028/s in absence of Ca2+, and 0.000025/s in presence of Ca2+ Streptomyces griseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.24 0.13
-
 L-valine-4-nitroanilide pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
3.4.11.24 0.26
-
 L-valine-4-nitroanilide pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
3.4.11.24 0.47
-
 L-alanine-4-nitroanilide pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
3.4.11.24 1.31
-
 L-leucine-4-nitroanilide pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
3.4.11.24 1.81
-
 L-alanine-4-nitroanilide pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
3.4.11.24 2.94
-
 L-alanine-4-nitroanilide pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.24 0.000016
-
 amastatin pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
3.4.11.24 0.0005
-
 L-leucine chloromethyl ketone pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
3.4.11.24 0.00055
-
 amastatin pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
3.4.11.24 0.0019
-
 L-phenylalanine chloromethyl ketone pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
3.4.11.24 0.0026
-
 bestatin pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
3.4.11.24 0.0053
-
 L-phenylalanine chloromethyl ketone pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
3.4.11.24 0.0054
-
 bestatin pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
3.4.11.24 0.0055
-
 L-leucine chloromethyl ketone pH 8.0, 30°C, in absence of Ca2+ Streptomyces griseus
3.4.11.24 8.7
-
 L-methionine pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
3.4.11.24 9.1
-
 L-methionine pH 6.5, 30°C, in presence of Ca2+ Streptomyces griseus
3.4.11.24 12.4
-
 L-leucine pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
3.4.11.24 12.7
-
 L-phenylalanine pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus
3.4.11.24 12.8
-
 L-leucine pH 6.5, 30°C, in presence of Ca2+ Streptomyces griseus
3.4.11.24 36.6
-
 D-phenylalanine pH 8.0, 30°C, in presence of Ca2+ Streptomyces griseus