EC Number | General Stability | Organism |
---|---|---|
3.4.17.B1 | 10% glycerol stabilizes | Saccharolobus solfataricus |
3.4.17.B1 | 13% remaining activity in 0.1% SDS at 70°C after 180 min, stable at 40°C | Saccharolobus solfataricus |
3.4.17.B1 | 40% remaining activity in 8 M urea after 55 min at 40°C | Saccharolobus solfataricus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.17.B1 | EDTA | complete inhibition, can be reversed by Zn2+ or to a lesser extent by Co2+ | Saccharolobus solfataricus | |
3.4.17.B1 | o-phenanthroline | - |
Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.17.B1 | 0.061 | - |
benzyloxycarbonyl-L-Arg | pH 6.5, 60°C | Saccharolobus solfataricus | |
3.4.17.B1 | 0.17 | - |
benzoyl-glycyl-arginine | pH 6.5, 60°C | Saccharolobus solfataricus | |
3.4.17.B1 | 0.22 | - |
Benzyloxycarbonyl-Asp | pH 6.5, 60°C | Saccharolobus solfataricus | |
3.4.17.B1 | 0.23 | - |
benzoyl-glycyl-L-lysine | pH 6.5, 60°C | Saccharolobus solfataricus | |
3.4.17.B1 | 0.31 | - |
benzyloxycarbonyl-Phe | pH 6.5, 60°C | Saccharolobus solfataricus | |
3.4.17.B1 | 0.69 | - |
benzyloxycarbonyl-Gly-Gly-Phe | pH 6.5, 60°C | Saccharolobus solfataricus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.17.B1 | intracellular | - |
Saccharolobus solfataricus | 5622 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.17.B1 | Co2+ | can partially substitute for Zn2+, activates | Saccharolobus solfataricus | |
3.4.17.B1 | additional information | Ni2+, Mn2+, Ca2+, and Mg2+ are ineffective | Saccharolobus solfataricus | |
3.4.17.B1 | Zn2+ | metalloprotease, dependent on | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.17.B1 | 42000 | - |
4 * 42000, SDS-PAGE | Saccharolobus solfataricus |
3.4.17.B1 | 170000 | - |
gel filtration | Saccharolobus solfataricus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.17.B1 | peptide + H2O | Saccharolobus solfataricus | - |
? | - |
? | |
3.4.17.B1 | peptide + H2O | Saccharolobus solfataricus P2 | - |
? | - |
? | |
3.4.17.B1 | protein + H2O | Saccharolobus solfataricus | - |
peptides | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.4.17.B1 | acetonitril | 93% remaining activity in 50% acetonitril at 40°C after 101 min, inactivation at 70°C | Saccharolobus solfataricus |
3.4.17.B1 | Ethanol | 90% remaining activity in pure ethanol at 40°C after 98 min, inactivation over this time period at 70°C, 2% remaining activity in 50% ethanol at 70°C | Saccharolobus solfataricus |
3.4.17.B1 | Methanol | 56% remaining activity in pure methanol at 40°C after 98 min, inactivation over this time period at 70°C, 11% remaining activity in 50% methanol at 70°C | Saccharolobus solfataricus |
3.4.17.B1 | tetrahydrofuran | 19% remaining activity in 50% tetrahydrofuran after 42 min at 40°C, nactivation at 70°C | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.17.B1 | Saccharolobus solfataricus | P80092 | strain MT-4, ATCC 49155 | - |
3.4.17.B1 | Saccharolobus solfataricus P2 | P80092 | strain MT-4, ATCC 49155 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.17.B1 | to electrophoretic homogeneity, 76fold | Saccharolobus solfataricus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.17.B1 | additional information | - |
substrate specificity, esterase activity | Saccharolobus solfataricus |
3.4.17.B1 | 4.1 | - |
purified enzyme, substrate benzyloxycarbonyl-Phe | Saccharolobus solfataricus |
3.4.17.B1 | 8.6 | - |
purified enzyme, substrate benzyloxycarbonyl-Asp | Saccharolobus solfataricus |
3.4.17.B1 | 16.3 | - |
purified enzyme, substrate benzyloxycarbonyl-Arg | Saccharolobus solfataricus |
3.4.17.B1 | 21.9 | - |
purified enzyme, substrate benzoyl-glycyl-arginine | Saccharolobus solfataricus |
3.4.17.B1 | 27.2 | - |
purified enzyme, substrate benzoyl-glycyl-lysine | Saccharolobus solfataricus |
3.4.17.B1 | 38.7 | - |
purified enzyme, substrate benzyloxycarbonyl-Gly-Gly-Phe | Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.17.B1 | benzoyl-glycyl-arginine + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.4.17.B1 | benzoyl-glycyl-L-lysine + H2O | - |
Saccharolobus solfataricus | benzoyl-glycine + L-lysine | - |
? | |
3.4.17.B1 | benzoyl-glycyl-lysine + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.4.17.B1 | benzyloxycarbonyl-Ala-Ser-methyl ester + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.4.17.B1 | benzyloxycarbonyl-Ala-Val-methyl ester + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.4.17.B1 | benzyloxycarbonyl-Arg + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.4.17.B1 | benzyloxycarbonyl-Asp + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.4.17.B1 | benzyloxycarbonyl-Glu-methyl ester + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.4.17.B1 | benzyloxycarbonyl-Gly-Gly-Phe + H2O | best substrate | Saccharolobus solfataricus | ? | - |
? | |
3.4.17.B1 | benzyloxycarbonyl-Gly-Gly-Phe + H2O | best substrate | Saccharolobus solfataricus P2 | ? | - |
? | |
3.4.17.B1 | benzyloxycarbonyl-L-Arg + H2O | - |
Saccharolobus solfataricus | benzyloxycarbonate + L-Arg | - |
? | |
3.4.17.B1 | benzyloxycarbonyl-Leu-Ala-methyl ester + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.4.17.B1 | benzyloxycarbonyl-Leu-Gly-methyl ester + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.4.17.B1 | benzyloxycarbonyl-Phe + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.4.17.B1 | benzyloxycarbonyl-Phe-Leu + H2O | - |
Saccharolobus solfataricus | benzyloxycarbonyl-Phe + Leu | - |
? | |
3.4.17.B1 | benzyloxycarbonyl-Phe-Leu + H2O | - |
Saccharolobus solfataricus P2 | benzyloxycarbonyl-Phe + Leu | - |
? | |
3.4.17.B1 | benzyloxycarbonyl-Tyr-ethyl ester + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.4.17.B1 | additional information | enzyme also shows esterase activity, no activity with 4-nitroanilide substrates | Saccharolobus solfataricus | ? | - |
? | |
3.4.17.B1 | additional information | enzyme also shows esterase activity, no activity with 4-nitroanilide substrates | Saccharolobus solfataricus P2 | ? | - |
? | |
3.4.17.B1 | peptide + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
3.4.17.B1 | peptide + H2O | broad substrate specificity, overview | Saccharolobus solfataricus | ? | - |
? | |
3.4.17.B1 | peptide + H2O | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
3.4.17.B1 | peptide + H2O | broad substrate specificity, overview | Saccharolobus solfataricus P2 | ? | - |
? | |
3.4.17.B1 | protein + H2O | - |
Saccharolobus solfataricus | peptides | - |
? | |
3.4.17.B1 | protein + H2O | broad substrate specificity, overview | Saccharolobus solfataricus | peptides | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.17.B1 | tetramer | 4 * 42000, SDS-PAGE | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.17.B1 | carboxypeptidase S1 | - |
Saccharolobus solfataricus |
3.4.17.B1 | M20.008 | Merops-ID | Saccharolobus solfataricus |
3.4.17.B1 | thermostable carboxypeptidase 1 | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.17.B1 | 60 | - |
assay at | Saccharolobus solfataricus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.17.B1 | 40 | 85 | linear increase, still active at room temperature | Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.17.B1 | - |
25 | instable at, glycerol stabilizes | Saccharolobus solfataricus |
3.4.17.B1 | 85 | - |
stable for 15 min | Saccharolobus solfataricus |
3.4.17.B1 | 90 | - |
85% remaining activity | Saccharolobus solfataricus |
3.4.17.B1 | 95 | - |
17% remaining activity | Saccharolobus solfataricus |
3.4.17.B1 | 100 | - |
inactivation | Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.17.B1 | 0.97 | - |
benzoyl-glycyl-arginine | pH 6.5, 60°C | Saccharolobus solfataricus | |
3.4.17.B1 | 3.4 | - |
benzyloxycarbonyl-Phe | pH 6.5, 60°C | Saccharolobus solfataricus | |
3.4.17.B1 | 6 | - |
Benzyloxycarbonyl-Asp | pH 6.5, 60°C | Saccharolobus solfataricus | |
3.4.17.B1 | 10.4 | - |
Benzyloxycarbonyl-Arg | pH 6.5, 60°C | Saccharolobus solfataricus | |
3.4.17.B1 | 16.6 | - |
benzoyl-glycyl-arginine | pH 6.5, 60°C | Saccharolobus solfataricus | |
3.4.17.B1 | 21.2 | - |
benzoyl-glycyl-lysine | pH 6.5, 60°C | Saccharolobus solfataricus | |
3.4.17.B1 | 36.4 | - |
benzyloxycarbonyl-Gly-Gly-Phe | pH 6.5, 60°C | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.17.B1 | 5.5 | 9 | broad optimum, assay at pH 6.5 | Saccharolobus solfataricus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.4.17.B1 | Saccharolobus solfataricus | isoelectric focusing | - |
5.9 |