Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Spungin, A.; Blumberg, S.
    Streptomyces griseus aminopeptidase is a calcium-activated zinc metalloprotein. Purification and properties of the enzyme (1989), Eur. J. Biochem., 183, 471-477.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.24 1,10-phenanthroline 50% inhibition at pI 4.9 Streptomyces griseus
3.4.11.24 EDTA complete inhibition, activity cannot be completely restored by addition of 1 mM CaCl2 alone but together with 0.0001 mM ZnCl2 by 90% Streptomyces griseus
3.4.11.24 EGTA complete inhibition, activity can be completely restored by addition of 1 mM CaCl2 Streptomyces griseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.24 Ca2+ activates about 6fold, non-cooperative binding Streptomyces griseus
3.4.11.24 Zn2+ metalloprotease, dependent on Streptomyces griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.24 additional information
-
 enzymes are retarded on the Superose gel Streptomyces griseus
3.4.11.24 21000
-
 API, gel filtration Streptomyces griseus
3.4.11.24 22500
-
 APII, gel filtration Streptomyces griseus
3.4.11.24 33000
-
 1 * 33000, API, SDS-PAGE, 1 * 34000, APII, SDS-PAGE Streptomyces griseus
3.4.11.24 34000
-
 1 * 33000, API, SDS-PAGE, 1 * 34000, APII, SDS-PAGE Streptomyces griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.24 peptide + H2O Streptomyces griseus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.24 Streptomyces griseus P80561
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.24 from commercial product, 2 peaks API and APII, endopeptidase is eliminated Streptomyces griseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.24 commercial preparation protease type XIV i.e. pronase E, a protease mixture form Streptomyces griseus Streptomyces griseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.24 306
-
 purified API Streptomyces griseus
3.4.11.24 317
-
 purified APII Streptomyces griseus
3.4.11.24 461
-
 purified API in presence of 1 mM CaCl2 Streptomyces griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.24 L-leucine-4-nitroanilide + H2O
-
 Streptomyces griseus L-leucine + 4-nitroaniline
-
 ?
3.4.11.24 peptide + H2O
-
 Streptomyces griseus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.24 monomer 1 * 33000, API, SDS-PAGE, 1 * 34000, APII, SDS-PAGE Streptomyces griseus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.24 AP
-
 Streptomyces griseus
3.4.11.24 M28.003 Merops-ID Streptomyces griseus
3.4.11.24 SGAP
-
 Streptomyces griseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.24 25
-
 assay at Streptomyces griseus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.24 69
-
 stable during heating for 5 h at 69°C as part of the purification process Streptomyces griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.24 8
-
 assay at Streptomyces griseus