Literature summary extracted from
Cho, S.K.; Hofmann, S.L.
pdf1, a palmitoyl protein thioesterase 1 ortholog in Schizosaccharomyces pombe: a yeast model of infantile Batten disease (2004), Eukaryot. Cell, 3, 302-310.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.2.22 |
DNA sequence determination and analysis, enzyme is encoded in frame with a second gene coding for an indispensable dolichol pyrophosphatase, gene pdf1, strains bearing a deletion of the entire pdf1 open reading frame are nonviable, due to Dolpp1p deficiency, and are rescued by pdf1 expression plasmid, mutations only in the palmitoyl protein thioesterase 1 domain results in cells abnormally sensitive to sodium vanadate and elevated extracellular pH, the pdf1-deficient phenotype is complemented by the human PPT1 gene |
Schizosaccharomyces pombe |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.2.22 |
Sodium vanadate |
pdf1 mutant |
Schizosaccharomyces pombe |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
3.1.2.22 |
lysosome |
- |
Schizosaccharomyces pombe |
5764 |
- |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.1.2.22 |
31000 |
- |
x * 31000, processed enzyme, SDS-PAGE, x * 101000, unprocessed enzyme, SDS-PAGE |
Schizosaccharomyces pombe |
3.1.2.22 |
101000 |
- |
x * 31000, processed enzyme, SDS-PAGE, x * 101000, unprocessed enzyme, SDS-PAGE |
Schizosaccharomyces pombe |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.1.2.22 |
additional information |
Schizosaccharomyces pombe |
PPT1 mutations cause severe neurodegenerative storage disorder, termed infantile Batten, in humans, Schizosaccharomyces pombe is a genetically tractable model for the sutdy of this disease due to evolutionary highly conserved gene and function |
? |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.2.22 |
Schizosaccharomyces pombe |
O59747 |
cosmid; isozyme PPT1. gene pdf1 |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
3.1.2.22 |
proteolytic modification |
enzyme contains 2 putative cleavage sites for the kex-related endopeptidase Krp1p, the precursor is proteolytically processed to form distinct Ppt1p and Dolpp1p domains, Arg354 is crucial for the processing |
Schizosaccharomyces pombe |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
3.1.2.22 |
palmitoyl[protein] + H2O = palmitate + protein |
specific for long-chain thioesters of fatty acids from S-acylated residues in proteins, palmitoyl cysteine and palmitoyl-CoA |
Schizosaccharomyces pombe |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.2.22 |
4-methylumbelliferyl 6-S-palmitoyl-6-thio-beta-D-glucoside + H2O |
artificial substrate |
Schizosaccharomyces pombe |
4-methylumbelliferyl 6-thio-beta-D-glucoside + palmitate |
- |
? |
|
3.1.2.22 |
additional information |
PPT1 mutations cause severe neurodegenerative storage disorder, termed infantile Batten, in humans, Schizosaccharomyces pombe is a genetically tractable model for the sutdy of this disease due to evolutionary highly conserved gene and function |
Schizosaccharomyces pombe |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.1.2.22 |
? |
x * 31000, processed enzyme, SDS-PAGE, x * 101000, unprocessed enzyme, SDS-PAGE |
Schizosaccharomyces pombe |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.2.22 |
palmitoyl protein thioesterase 1 |
- |
Schizosaccharomyces pombe |
3.1.2.22 |
PPT1 |
- |
Schizosaccharomyces pombe |