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Literature summary extracted from

  • Cho, S.K.; Hofmann, S.L.
    pdf1, a palmitoyl protein thioesterase 1 ortholog in Schizosaccharomyces pombe: a yeast model of infantile Batten disease (2004), Eukaryot. Cell, 3, 302-310.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.22 DNA sequence determination and analysis, enzyme is encoded in frame with a second gene coding for an indispensable dolichol pyrophosphatase, gene pdf1, strains bearing a deletion of the entire pdf1 open reading frame are nonviable, due to Dolpp1p deficiency, and are rescued by pdf1 expression plasmid, mutations only in the palmitoyl protein thioesterase 1 domain results in cells abnormally sensitive to sodium vanadate and elevated extracellular pH, the pdf1-deficient phenotype is complemented by the human PPT1 gene Schizosaccharomyces pombe

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.22 Sodium vanadate pdf1 mutant Schizosaccharomyces pombe

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.22 lysosome
-
Schizosaccharomyces pombe 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.22 31000
-
x * 31000, processed enzyme, SDS-PAGE, x * 101000, unprocessed enzyme, SDS-PAGE Schizosaccharomyces pombe
3.1.2.22 101000
-
x * 31000, processed enzyme, SDS-PAGE, x * 101000, unprocessed enzyme, SDS-PAGE Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.22 additional information Schizosaccharomyces pombe PPT1 mutations cause severe neurodegenerative storage disorder, termed infantile Batten, in humans, Schizosaccharomyces pombe is a genetically tractable model for the sutdy of this disease due to evolutionary highly conserved gene and function ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.22 Schizosaccharomyces pombe O59747 cosmid; isozyme PPT1. gene pdf1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.2.22 proteolytic modification enzyme contains 2 putative cleavage sites for the kex-related endopeptidase Krp1p, the precursor is proteolytically processed to form distinct Ppt1p and Dolpp1p domains, Arg354 is crucial for the processing Schizosaccharomyces pombe

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.2.22 palmitoyl[protein] + H2O = palmitate + protein specific for long-chain thioesters of fatty acids from S-acylated residues in proteins, palmitoyl cysteine and palmitoyl-CoA Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.22 4-methylumbelliferyl 6-S-palmitoyl-6-thio-beta-D-glucoside + H2O artificial substrate Schizosaccharomyces pombe 4-methylumbelliferyl 6-thio-beta-D-glucoside + palmitate
-
?
3.1.2.22 additional information PPT1 mutations cause severe neurodegenerative storage disorder, termed infantile Batten, in humans, Schizosaccharomyces pombe is a genetically tractable model for the sutdy of this disease due to evolutionary highly conserved gene and function Schizosaccharomyces pombe ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.2.22 ? x * 31000, processed enzyme, SDS-PAGE, x * 101000, unprocessed enzyme, SDS-PAGE Schizosaccharomyces pombe

Synonyms

EC Number Synonyms Comment Organism
3.1.2.22 palmitoyl protein thioesterase 1
-
Schizosaccharomyces pombe
3.1.2.22 PPT1
-
Schizosaccharomyces pombe