EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.B19 | expression of wild-type and mutant E41Q in deficient mutant cell DELTAyme1, complementation only by the wild-type | Saccharomyces cerevisiae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.24.B18 | E614Q | site-directed mutagenesis, exchange of active site residue, stongly reduced activity | Saccharomyces cerevisiae |
3.4.24.B19 | E541Q | site-directed mutagenesis, exchange in the active site, no complementation of deficient mutant cell DELTAyme1 | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.B19 | EDTA | strong | Saccharomyces cerevisiae | |
3.4.24.B19 | o-phenanthroline | strong | Saccharomyces cerevisiae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.24.B18 | mitochondrial inner membrane | inner side, exposes catalytic sites to the mitochondrial matrix | Saccharomyces cerevisiae | 5743 | - |
3.4.24.B18 | mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
3.4.24.B19 | mitochondrial inner membrane | integral, a large domain is exposed to the intermembrane space | Saccharomyces cerevisiae | 5743 | - |
3.4.24.B19 | mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.B18 | protein + H2O | Saccharomyces cerevisiae | - |
peptides | - |
? | |
3.4.24.B19 | protein + H2O | Saccharomyces cerevisiae | proteolytic activity of the i-AAA protease is required for the maintenance of mitochondrial function at high temperature, pointing to an important role in mitochondrial biogenesis | peptides | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.B18 | Saccharomyces cerevisiae | - |
in complex with Yme1p, EC 3.4.24.B19, forming i-AAA protease | - |
3.4.24.B19 | Saccharomyces cerevisiae | - |
in complex with m-AAA protease Yta12p, EC 3.4.24.B18, complex is termed i-AAA protease | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.4.24.B18 | proteolytic degradation of proteins | conserved metal-binding site HEXXH, selective degradation, mechanism | Saccharomyces cerevisiae | |
3.4.24.B19 | proteolytic degradation of proteins | conserved metal-binding site HEXXH, selective degradation, mechanism | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.B18 | hybrid protein + H2O | hybrid protein of subunit 2 of cytochrome oxidase residues 1-74, mouse dihydrofolate reductase, and mitochondrial presequence, residues 1-66, of subunit 9 of the ATPase of Neurospora crassa, in vitro import into the mitochondrion | Saccharomyces cerevisiae | 2 peptide fragments f2 and f3 | product characterization | ? | |
3.4.24.B18 | additional information | Yta12p enzyme requires YTA10-12 complex formation for activity | Saccharomyces cerevisiae | ? | - |
? | |
3.4.24.B18 | protein + H2O | - |
Saccharomyces cerevisiae | peptides | - |
? | |
3.4.24.B18 | residues 1-74 of subunit 2 of cytochrome oxidase + H2O | two-step procedure, in vitro import into the mitochondrion | Saccharomyces cerevisiae | ? | - |
? | |
3.4.24.B19 | hybrid protein of subunit 2 of cytochrome oxidase residues 1-74, mouse dihydrofolate reductase, and mitochondrial presequence, residues 1-66, of subunit 9 of the ATPase of Neurospora crassa + H2O | in vitro import into the mitochondrion | Saccharomyces cerevisiae | 4 peptide fragments f1-f4 | product characterization | ? | |
3.4.24.B19 | protein + H2O | proteolytic activity of the i-AAA protease is required for the maintenance of mitochondrial function at high temperature, pointing to an important role in mitochondrial biogenesis | Saccharomyces cerevisiae | peptides | - |
? | |
3.4.24.B19 | residues 1-74 of subunit 2 of cytochrome oxidase + H2O | two-step procedure, in vitro import into the mitochondrion | Saccharomyces cerevisiae | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.B18 | M41.003 | Merops-ID | Saccharomyces cerevisiae |
3.4.24.B18 | YTA12p | - |
Saccharomyces cerevisiae |
3.4.24.B19 | AAA protease | - |
Saccharomyces cerevisiae |
3.4.24.B19 | M41.004 | Merops-ID | Saccharomyces cerevisiae |
3.4.24.B19 | YME1 | - |
Saccharomyces cerevisiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.B18 | 25 | - |
assay at | Saccharomyces cerevisiae |
3.4.24.B19 | 25 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.B18 | ATP | dependent on | Saccharomyces cerevisiae | |
3.4.24.B19 | ATP | dependent on | Saccharomyces cerevisiae |