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Literature summary extracted from

  • Kim, H.E.; Park, K.R.
    Purification and characterization of an esterase from Acinetobacter lwoffii I6C-1 (2002), Curr. Microbiol., 44, 401-405.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 Ca2+
-
 Acinetobacter lwoffii
3.1.1.1 Co2+
-
 Acinetobacter lwoffii
3.1.1.1 Cu2+
-
 Acinetobacter lwoffii
3.1.1.1 diisopropyl fluorophosphate
-
 Acinetobacter lwoffii
3.1.1.1 EDTA
-
 Acinetobacter lwoffii
3.1.1.1 Fe2+
-
 Acinetobacter lwoffii
3.1.1.1 Hg2+
-
 Acinetobacter lwoffii
3.1.1.1 Mg2+
-
 Acinetobacter lwoffii
3.1.1.1 Mn2+
-
 Acinetobacter lwoffii
3.1.1.1 PMSF
-
 Acinetobacter lwoffii
3.1.1.1 Zn2+
-
 Acinetobacter lwoffii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.011
-
 4-nitrophenyl butyrate pH 8.0, 37°C Acinetobacter lwoffii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.1 cytosol
-
 Acinetobacter lwoffii 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 45000
-
 approximately, gel filtration Acinetobacter lwoffii

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Acinetobacter lwoffii
-
 enzyme EstA
-
3.1.1.1 Acinetobacter lwoffii I6C-1
-
 enzyme EstA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 24.2fold Acinetobacter lwoffii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 1440
-
 purified enzyme Acinetobacter lwoffii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl butyrate + H2O
-
 Acinetobacter lwoffii 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O
-
 Acinetobacter lwoffii I6C-1 4-nitrophenol + butyrate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 monomer 1 * 45000, SDS-PAGE Acinetobacter lwoffii

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 EstA
-
 Acinetobacter lwoffii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 37
-
-
 Acinetobacter lwoffii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.1 20 48 about 50% of maximal activity at 20°C and 48°C Acinetobacter lwoffii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 50
-
 at least 1 h, stable Acinetobacter lwoffii
3.1.1.1 60
-
 50 min, 50% remaining activity Acinetobacter lwoffii
3.1.1.1 70
-
 40 min, 50% remaining activity Acinetobacter lwoffii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 8
-
-
 Acinetobacter lwoffii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 7 9.5 50% of maximal activity at pH 7.0 and pH 9.5 Acinetobacter lwoffii