Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kawamura, T.; Oda, T.; Muramatsu, T.
    Purification and characterization of a dipeptidyl carboxypeptidase from the polychaete Neanthes virens resembling angiotensin I converting enzyme (2000), Comp. Biochem. Physiol. B, 126, 29-37.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.15.1 Diazoacetylnorleucine methyl ester 1.0 mM, activation to 115% of control Alitta virens
3.4.15.1 diisopropylphosphoryl fluoride 1.0 mM, activation to 116.3% of control Alitta virens
3.4.15.1 Soybean trypsin inhibitor 0.5 mM, activation to 115% of control Alitta virens
3.4.15.1 tosylphenylalanine chloromethyl ketone 1.0 mM, activation to 113% of control Alitta virens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.15.1 1,10-phenanthroline 5.0 mM, complete inhibition Alitta virens
3.4.15.1 antipain 0.1 mg/ml, 26% inhibition Alitta virens
3.4.15.1 captopril 0.1 mM, complete inhibition Alitta virens
3.4.15.1 CuCl2 73% inhibition at 0.1 mM, 99% inhibition at 1.0 mM Alitta virens
3.4.15.1 EDTA 1.0 mM, complete inhibition Alitta virens
3.4.15.1 leupeptin 0.1 mg/ml, 18% inhibition Alitta virens
3.4.15.1 lisinopril
-
 Alitta virens
3.4.15.1 additional information enzyme is not affected by addition of 0.1-1.0 mM CaCl2, Mn2Cl2, MgCl2 or 0.1 mM ZnCl2 Alitta virens
3.4.15.1 PCMB 1.0 mM, 42% inhibition Alitta virens
3.4.15.1 ZnCl2 1.0 mM, 98% inhibition Alitta virens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.15.1 0.56
-
 benzoyl-Gly-His-Leu
-
 Alitta virens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.15.1 CoCl2 0.1-1.0 mM, 2fold activation Alitta virens
3.4.15.1 additional information enzyme is not affected by addition of 0.1-1.0 mM CaCl2, Mn2Cl2, MgCl2 or 0.1 mM ZnCl2 Alitta virens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.15.1 69000
-
 gel filtration Alitta virens
3.4.15.1 71000
-
 1 * 71000, SDS-PAGE Alitta virens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.15.1 angiotensin I + H2O Alitta virens the enzyme plays an important role in blood pressure homeostasis angiotensin II + His-Leu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.15.1 Alitta virens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.15.1
-
 Alitta virens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.15.1 466.8
-
-
 Alitta virens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.15.1 angiotensin I + H2O
-
 Alitta virens angiotensin II + His-Leu
-
 ?
3.4.15.1 angiotensin I + H2O the enzyme plays an important role in blood pressure homeostasis Alitta virens angiotensin II + His-Leu
-
 ?
3.4.15.1 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O cleavage sites: Arg-Pro-/-Lys-Pro-/-Gln-Gln-/-Phe-Phe-/-Gly-Leu-Met-NH2 Alitta virens ?
-
 ?
3.4.15.1 bradykinin + H2O
-
 Alitta virens Phe-Arg + Ser-Pro + Arg-Pro-Pro-Gly-Phe
-
 ?
3.4.15.1 hippuryl-Gly-His-Leu + H2O
-
 Alitta virens hippuric acid + His-Leu
-
 ?
3.4.15.1 hippuryl-His-Leu + H2O
-
 Alitta virens hippuric acid + His-Leu
-
 ?
3.4.15.1 additional information no cleavage of imido-bonds. No hydrolysis of angiotensin II and angiotensin III Alitta virens ?
-
 ?
3.4.15.1 Tyr-Gly-Gly-Phe-Leu + H2O
-
 Alitta virens Tyr-Gly-Gly + Phe-Leu
-
 ?
3.4.15.1 Tyr-Gly-Gly-Phe-Met + H2O
-
 Alitta virens Tyr-Gly-Gly + Phe-Met
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.15.1 monomer 1 * 71000, SDS-PAGE Alitta virens

Synonyms

EC Number Synonyms Comment Organism
3.4.15.1 DCP
-
 Alitta virens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.15.1 45
-
-
 Alitta virens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.15.1 345
-
 benzoyl-Gly-His-Leu
-
 Alitta virens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.15.1 8
-
-
 Alitta virens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.15.1 0.00000138
-
 captopril
-
 Alitta virens
3.4.15.1 0.00000207
-
 lisinopril
-
 Alitta virens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.15.1 Alitta virens
-
-
 4.5