BRENDA - Enzyme Database

Characterization of a renal medium chain acyl-CoA synthetase responsible for glycine conjugation in mouse kidney mitochondria

Kasuya, F.; Igarashi, K.; Fukui, M.; Chem. Biol. Interact. 118, 233-246 (1999)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.2
2-hydroxydodecanoic acid
0.04 mM, 48% inhibition
Mus musculus
6.2.1.2
diflunisal
0.002 mM, 55% inhibition
Mus musculus
6.2.1.2
salicylic acid
0.05 mM, 53% inhibition
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.2.1.2
mitochondrion
-
Mus musculus
5739
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.2.1.2
additional information
Mus musculus
the enzyme is responsible for glycine conjugation
?
-
Mus musculus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.2.1.2
Mus musculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.2.1.2
-
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
6.2.1.2
kidney
-
Mus musculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
6.2.1.2
4.15
-
-
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.2
ATP + 1-naphthoic acid + CoA
less than 10% of the activity with hexanoic acid
650831
Mus musculus
AMP + diphosphate + 1-naphthoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 1-naphthylacetic acid + CoA
about 25% of the activity with hexanoic acid
650831
Mus musculus
AMP + diphosphate + 1-naphthyl-acetyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 2-naphthylacetic acid + CoA
about 35% of the activity with hexanoic acid
650831
Mus musculus
AMP + diphosphate + 2-naphthyl-acetyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 3-aminobenzoate + CoA
29% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 3-aminobenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 3-chlorobenzoate + CoA
126% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 3-chlorobenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 3-methoxybenzoate + CoA
54% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 3-methoxybenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 3-methylbenzoate + CoA
138% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 3-methylbenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 3-nitrobenzoate + CoA
28% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 3-nitrobenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 4-aminobenzoate + CoA
19% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 4-aminobenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 4-chlorobenzoate + CoA
96% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 4-chlorobenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 4-methoxybenzoate + CoA
43% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 4-methoxybenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 4-methylbenzoate + CoA
114% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 4-methylbenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 4-nitrobenzoate + CoA
29% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 4-nitrobenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + benzoate + CoA
about 30% of the activity with hexanoic acid
650831
Mus musculus
AMP + diphosphate + benzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + hexanoate + CoA
-
650831
Mus musculus
AMP + diphosphate + hexanoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + indomethacin + CoA
less than 5% of the activity with hexanoic acid
650831
Mus musculus
AMP + diphosphate + ?
-
650831
Mus musculus
?
6.2.1.2
ATP + octanoate + CoA
about 50% of the activity with hexanoic acid
650831
Mus musculus
AMP + diphosphate + octanoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + tranexamic acid + CoA
less than 10% of the activity with hexanoic acid
650831
Mus musculus
AMP + diphosphate + tranexoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + valproic acid + CoA
less than 10% of the activity with hexanoic acid
650831
Mus musculus
AMP + diphosphate + valproyl-CoA
-
650831
Mus musculus
?
6.2.1.2
additional information
the enzyme is responsible for glycine conjugation
650831
Mus musculus
?
-
650831
Mus musculus
?
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.2
2-hydroxydodecanoic acid
0.04 mM, 48% inhibition
Mus musculus
6.2.1.2
diflunisal
0.002 mM, 55% inhibition
Mus musculus
6.2.1.2
salicylic acid
0.05 mM, 53% inhibition
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.2.1.2
mitochondrion
-
Mus musculus
5739
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.2.1.2
additional information
Mus musculus
the enzyme is responsible for glycine conjugation
?
-
Mus musculus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.2.1.2
-
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
6.2.1.2
kidney
-
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
6.2.1.2
4.15
-
-
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.2
ATP + 1-naphthoic acid + CoA
less than 10% of the activity with hexanoic acid
650831
Mus musculus
AMP + diphosphate + 1-naphthoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 1-naphthylacetic acid + CoA
about 25% of the activity with hexanoic acid
650831
Mus musculus
AMP + diphosphate + 1-naphthyl-acetyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 2-naphthylacetic acid + CoA
about 35% of the activity with hexanoic acid
650831
Mus musculus
AMP + diphosphate + 2-naphthyl-acetyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 3-aminobenzoate + CoA
29% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 3-aminobenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 3-chlorobenzoate + CoA
126% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 3-chlorobenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 3-methoxybenzoate + CoA
54% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 3-methoxybenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 3-methylbenzoate + CoA
138% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 3-methylbenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 3-nitrobenzoate + CoA
28% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 3-nitrobenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 4-aminobenzoate + CoA
19% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 4-aminobenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 4-chlorobenzoate + CoA
96% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 4-chlorobenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 4-methoxybenzoate + CoA
43% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 4-methoxybenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 4-methylbenzoate + CoA
114% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 4-methylbenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + 4-nitrobenzoate + CoA
29% of the activity with benzoate
650831
Mus musculus
AMP + diphosphate + 4-nitrobenzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + benzoate + CoA
about 30% of the activity with hexanoic acid
650831
Mus musculus
AMP + diphosphate + benzoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + hexanoate + CoA
-
650831
Mus musculus
AMP + diphosphate + hexanoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + indomethacin + CoA
less than 5% of the activity with hexanoic acid
650831
Mus musculus
AMP + diphosphate + ?
-
650831
Mus musculus
?
6.2.1.2
ATP + octanoate + CoA
about 50% of the activity with hexanoic acid
650831
Mus musculus
AMP + diphosphate + octanoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + tranexamic acid + CoA
less than 10% of the activity with hexanoic acid
650831
Mus musculus
AMP + diphosphate + tranexoyl-CoA
-
650831
Mus musculus
?
6.2.1.2
ATP + valproic acid + CoA
less than 10% of the activity with hexanoic acid
650831
Mus musculus
AMP + diphosphate + valproyl-CoA
-
650831
Mus musculus
?
6.2.1.2
additional information
the enzyme is responsible for glycine conjugation
650831
Mus musculus
?
-
650831
Mus musculus
?